E3 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100285

Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640221

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100285 represents NCBI reference of YP_002640221 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGTATCTTTCTCCTCTTCCTGTTCAGTCTCCCCAGTGGCCTGTATGCCCAGACTGCCGAGCGCC
  CCTTGAAGGTTGTTGTAGAGGCCGGGCACAATGTAACACTCCCCCACCTGTCAGGGAGTCACCAGACCGG
  CCACGTGACCTGGCTGGTCGAGACCAGCGACTATGGATCTGCGAGTCCTGATAATTTTATCTTTTCCGGA
  CAGAAGCTGTGTCAGTTTACAGATAGAACAATGGTTTGGCCGTATTACAATCTGCACTTTAACTGCGAGA
  ACTACGATCTGAACCTTTTCTGGCTGAAAGTAGAGAACTCCGCCATCTATAATGTGAAAAATACAGTGAA
  TGCCTCAGAAACGAACATCTATTATGACCTGAGGGTCGTCCAAATATTCCCTCCCAAGTGCATCATCACC
  TCTAAGTACCTGACGAACGATTACTGTCATATTACCATCAACTGCACCAACTCTGATTACCCCAATAAGG
  TAGTCTTCAATAACGTCTCACGGTGGTATTATGGGTACGGCAAAGGCAGCCCTACCCTGCCAAATTACTT
  CATCACAAACTTCAATGTGAGTGGCATTACAAAGAGCTTTAATCACACCTACCCGTTCAATGAACTCTGC
  GACTATCCTACCTCCCAATCACAACACTCTCTCACACACACGGTTTCTACTGTTATTTTTCTTGGAATTA
  TTGGCTTCTCCATTCTGATCATCATCGCGGCCTTTATCTATTTGTGTTGGCACCGAAAAAGCCTGTGCGT
  CAGCAAAACTGAGCCACTCATGCCAATCCCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100285 representing YP_002640221
  Red=Cloning sites Green=Tags
  
  MLSIFLLFLFSLPSGLYAQTAERPLKVVVEAGHNVTLPHLSGSHQTGHVTWLVETSDYGSASPDNFIFSG
  QKLCQFTDRTMVWPYYNLHFNCENYDLNLFWLKVENSAIYNVKNTVNASETNIYYDLRVVQIFPPKCIIT
  SKYLTNDYCHITINCTNSDYPNKVVFNNVSRWYYGYGKGSPTLPNYFITNFNVSGITKSFNHTYPFNELC
  DYPTSQSQHSLTHTVSTVIFLGIIGFSILIIIAAFIYLCWHRKSLCVSKTEPLMPIPY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 804 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, YP_002640221
• RefSeq ORF: 804
• Locus ID: 1460862
• MW: 30.7 kDa