E2A (NC_001460) Virus Tagged ORF Clone

CAT#: VC100270

Myc-DDK-tagged ORF clone of viral ORF for E2A-L gene product [Human adenovirus A], codon optimized for human cell expression, NP_040926

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100270 represents NCBI reference of NP_040926 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTAACCAACATTCTCAGCGCGAGAGAACACCCGATAGAAGCGCGCAGCCCCCACCTCCCAAAA
  TGGGGAGGTATTTTCTTGACTCTGAGTCCGAAGAGGAACTGGAAGCCGTTCCACTGCCTCCAAAAAAAAA
  AGTCAAAAAAAGTATGGCCGCCATACCTCTGAGCCCAGAAAGCGCTGAAGAGGAAGAGGCCGAACCCCCC
  AGGGCCGTCCTTGGGGTGATGGGCTTCTCTATGCCTCCTGTCCGGATCATGCACCATGCCGACGGCAGTC
  AATCCTTCCAGAAGATGGAAACAAGACAAGTTCATGTGCTGAAAGCTTCCGCCCAGAATTCTGACGAGAA
  TGAGAAAAATGTGGTCGTTGTGAGGAACCCAGCCAGTCAGCCCCTGGTTTCCGCTTGGGAGAAAGGAATG
  GAAGCAATGGCAATGCTGATGGAGAAATATCACGTGGACCATGATGAGAGAGCAACATTTCGGTTTCTTC
  CTGACCAGGGTTCCGTCTATAAAAAAATCTGCACAACCTGGCTGAATGAGGAAAAAAGAGGTCTCCAGCT
  CACATTCTCTAGTCAAAAGACTTTTCAGGAACTCATGGGACGGTTCCTGCAAGGCTACATGCAGGCGTAC
  GCTGGTGTGCAACAGAACAGCTGGGAACCCACCGGGTGTTGCGTATGGGAACACAAGTGTACTGAGAGGG
  AGGGTGAACTCCGATGCTTGCATGGTATGGAGATGGTAAGGAAGGAACATCTTGTTGAGATGGACGTTAC
  TTCCGAAAGTGGACAACGCGCCCTTAAAGAGAACCCTTCTAAAGCTAAAGTGGCCCAAAACAGATGGGGC
  CGAAACGTGGTGCAAATAAAGAACGACGACGCTCGGTGTTGTTTCCATGATGTGGGGTGCGGCAATAACA
  GTTTTTCTGGCAAGAGCTGCGGACTCTTTTACTCCGAGGGGATGAAAGCACAGATCGCCTTTAGACAGAT
  CGAGGCCTTCATGCTGGCCGACTATCCACATATGAGGCACGGCCAAAAGCGCCTTCTGATGCCCGTACGG
  TGCGAGTGTCTGAACAAACAGGATGGACTGCCTAGAATGGGGCGCCAGTTGTGCAAAATCACCCCCTTTA
  ACCTCTCTAACGTAGACAATATAGACATTAACGAAGTTACCGATCCCGGAGCTCTGGCATCCATCAAATA
  TCCGTGTCTGTTGGTCTTCCAGTGCGCAAATCCAGTGTACCGGAATGCACGGGGAAACGCAGGTCCAAAC
  TGTGATTTCAAAATAAGTGCCCCTGATGTGATGGGAGCACTGCAGCTGGTTCGACAGCTCTGGGGTGAAA
  ATTTTGACGGATCTCCCCCGCGGCTTGTGATTCCCGAGTTTAAATGGCACCAGCGGCTTCAGTACAGAAA
  TATCAGTCTCCCCACCAATCATGGGGACTGTCGGGAGGAGCCTTTCGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100270 representing NP_040926
  Red=Cloning sites Green=Tags
  
  MASNQHSQRERTPDRSAQPPPPKMGRYFLDSESEEELEAVPLPPKKKVKKSMAAIPLSPESAEEEEAEPP
  RAVLGVMGFSMPPVRIMHHADGSQSFQKMETRQVHVLKASAQNSDENEKNVVVVRNPASQPLVSAWEKGM
  EAMAMLMEKYHVDHDERATFRFLPDQGSVYKKICTTWLNEEKRGLQLTFSSQKTFQELMGRFLQGYMQAY
  AGVQQNSWEPTGCCVWEHKCTEREGELRCLHGMEMVRKEHLVEMDVTSESGQRALKENPSKAKVAQNRWG
  RNVVQIKNDDARCCFHDVGCGNNSFSGKSCGLFYSEGMKAQIAFRQIEAFMLADYPHMRHGQKRLLMPVR
  CECLNKQDGLPRMGRQLCKITPFNLSNVDNIDINEVTDPGALASIKYPCLLVFQCANPVYRNARGNAGPN
  CDFKISAPDVMGALQLVRQLWGENFDGSPPRLVIPEFKWHQRLQYRNISLPTNHGDCREEPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040926
• RefSeq ORF: 1452
• Locus ID: 1460850
• MW: 55.0 kDa