E2B (NC_001460) Virus Tagged ORF Clone

CAT#: VC100261

Myc-DDK-tagged ORF clone of viral ORF for E2B gene product [Human adenovirus A], codon optimized for human cell expression, NP_040917

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100261 represents NCBI reference of NP_040917 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGTCAATCCAGGATTGTGCAAGACTGACTGGTCAGAGCGTCGCCACCATAGAAAGATTCCTGC
  CATTGCGCAACATCTGGAATCGGGCCAGAGAATTCATGAGGGCAACGACCACGGCCGCCGGCATCACATG
  GATGTCCCGGTATATATACGAGTACCACAGGCTTATGCTGGAGGACCTGGCACCAGGCGCAGCTGCTACC
  CTGGGATGGCCTCTGTATCGCGAGCCACCCCCACACTTCCTGGTTGGGTACCAGTACCTTGTGAGAACCT
  GCAATGATTATGTCTTTGATAGTAGAGCCTATTCTCGCCTGCGGTATACAGAGACAGTGCAGCCGGGAAC
  GCAGACCGTGAACTGGAGCGTTATGACAAACTGCTCATATACTATAAACGCCGGAGCCTATCACAGGTTC
  GTCGACGTCGACGACTTTGCTACCACACTGACCCAGATCCAGCAGGCAGTCTTGGCCGAAAGGGTTGTAG
  CAGATCTGGCACTCCTGCAGCCCTTGCAGGGATATGGGAGCACACACATGGCAGACCGGGGGAACCGGGA
  GGTTGAAGTGGAGAGACTGATGCAGGACTACTACAAGGACCTTGGCCGGTGCCAGAGTGACGTGTGGGGG
  ATGGCAGACAGACTGCGAATCCAGCAGGCGGGGCCGAAAGATGTTGTGCTCCTGAAGACAATCCGAGGAC
  TCAAAACAGCCTACTTTAATTACATAATCTCCTCTATCGCACAGCTGCCCGGCACACTCGAGGAAACGAA
  ACTTAGCCTCCCCTGTGACTGTGACTGGCTCGACGCCTTTCTCGAGCGGTTTAGTGACCCTGTCGACATG
  CAAACGCTCAGCTCCCTTCGGGGCGTGCCTACTCAACAAATCATTAAGTGCATCATCAGCGCCGTGTCCT
  TGCCTAATGCTCCAAGGAATGCCTCATTTCCTATCCTGCACGAAACCGAGCTGCGCGGAGGCGTGTTTGA
  ACTTAGGCCCCGCGAAAATGGTAGGGCGGTGACAGAAACCATGCGGCGGCGGAGGGGCGAGATGATCGAG
  AGATTCGTGGATAGACTGCCAGTGAGACGACGCCGACGGAGACAGCCGCCTCCTCCCGTGGTTGAGGAGG
  AAATCATGGAAGTTGAGGAGGAGGAACAGGAGATCGTGCCTGGAGCCTTCCAGCAGGAAGTCCGCGAAAC
  TGTCGCTGAGCTGATCAGACTGCTTGAGGAAGAGCTGACTGTGGCAGCACGGAATTCACAGTTCTTCAAT
  TTTGCAGTTGACTTCTATGAGGCCATGGAGCGGCTCGAAGCCCTGGGTGATATTAACGAGCTCACCCTGA
  GGAGGTGGATCTTGTACTTCTTTGTAAGCGAACACGTGGCCACCACACTTAATTACCTGTTTCAGAGACT
  TCGCAATTACGGCGTTTTTGCCCGCCTGGTGGAGCTGAATTTGGCTCAGGTAGTTATGAGAGCGCGAGAT
  TCAGAGGGGGGCGTAGTGTATTCAAGGGTGTGGAACGAGATCGGATTGAATGCCTTCTCACAGCTGATGT
  CAAGGATCTCCAACGATCTCGCCGCCACCATCGAGAGAGCAGGACACGGAGACTTGCAGGAGGAAGAGAT
  TGACCAGCTCATGGCTGAAATTGCTTACCAGGACAACTCCGGGGACGTACAGGAGATACTCAGGCAGGCC
  GCCGTTAACGACACTGAAATCGACAGTGTGGAACTCTCTTTTAGATTTAAGACCACCGGACCGGTAGTGT
  TCACCCAGAGGCGACAGATACAGGACATAAATCGCAGGGTGGTTGCGCACGCGAGCCAGTTGAGAGCGCA
  ACACCTCCCCCTGCCAGAAAGGCAAGCGGATATCCCACTGCCCCCCTTGCCAGCTGGGCCTGAGCCACCC
  TTGCCACCTGGCGCCCGGCCGAGGAGGAGGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100261 representing NP_040917
  Red=Cloning sites Green=Tags
  
  MALSIQDCARLTGQSVATIERFLPLRNIWNRAREFMRATTTAAGITWMSRYIYEYHRLMLEDLAPGAAAT
  LGWPLYREPPPHFLVGYQYLVRTCNDYVFDSRAYSRLRYTETVQPGTQTVNWSVMTNCSYTINAGAYHRF
  VDVDDFATTLTQIQQAVLAERVVADLALLQPLQGYGSTHMADRGNREVEVERLMQDYYKDLGRCQSDVWG
  MADRLRIQQAGPKDVVLLKTIRGLKTAYFNYIISSIAQLPGTLEETKLSLPCDCDWLDAFLERFSDPVDM
  QTLSSLRGVPTQQIIKCIISAVSLPNAPRNASFPILHETELRGGVFELRPRENGRAVTETMRRRRGEMIE
  RFVDRLPVRRRRRRQPPPPVVEEEIMEVEEEEQEIVPGAFQQEVRETVAELIRLLEEELTVAARNSQFFN
  FAVDFYEAMERLEALGDINELTLRRWILYFFVSEHVATTLNYLFQRLRNYGVFARLVELNLAQVVMRARD
  SEGGVVYSRVWNEIGLNAFSQLMSRISNDLAATIERAGHGDLQEEEIDQLMAEIAYQDNSGDVQEILRQA
  AVNDTEIDSVELSFRFKTTGPVVFTQRRQIQDINRRVVAHASQLRAQHLPLPERQADIPLPPLPAGPEPP
  LPPGARPRRRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040917
• RefSeq ORF: 1923
• Locus ID: 1460849
• MW: 73.5 kDa