E4 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100288

Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640223

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100288 represents NCBI reference of YP_002640223 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCAAAGATCTCTTGTGCACTACAGCGTGTTGTTTCCTGAGAGTCTGAGGAATTACCTGCACGGGC
  TCGACTTCGAGGTGGTCACTTTCCTCAAGGACGTGTTGCCAGAGTTTTGGCTTTTGGTCATGCATTACCT
  GACCCCCCCCATGCGGGATGTTTACGTCGGGGCCACTCTGACCAATATGGGGCCTTTTGTGCAGGTGGTG
  TGTAGCGTCGGAACTCCTGAGCTTGTACCGGGTGGAGAACTCTCCCTCTTGCTCGCCTCAGATCTGTATG
  ATTTCATTCAGCTGGCCTTGCGCTGTCAGCTGAGGGATCAGGGTGTCGAGCCGAACGTGAACCTCCTGAA
  CCTTCTTCAGGTCTTTGAAGACCCTGATTTTTTTCAGCAGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100288 representing YP_002640223
  Red=Cloning sites Green=Tags
  
  MFQRSLVHYSVLFPESLRNYLHGLDFEVVTFLKDVLPEFWLLVMHYLTPPMRDVYVGATLTNMGPFVQVV
  CSVGTPELVPGGELSLLLASDLYDFIQLALRCQLRDQGVEPNVNLLNLLQVFEDPDFFQQI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 393 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, YP_002640223
• RefSeq ORF: 393
• Locus ID: 1460865
• MW: 15.0 kDa