L4 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100272

Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040928

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100272 represents NCBI reference of NP_040928 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAAGATATTCCTACTCCCTATATGTGGAGTTTTCAGCCCCAGATGGGCCTGGCTGCAGGGGCCG
  CGCAAGACTATTCAAGTAAGATGAACTGGCTGTCCGCTGGACCACATATGATTTCACGGGTGAATGGAGT
  GCGAGCAAGGCGCAATCAGATCCTCCTCGAACAGGCCGCTTTGACAGCCACCCCCAGGAATCAATTGAAT
  CCACCCAGCTGGCCCGCCGCCCTGATTTATCAAGAAAACCCACCGCCTACTACAGTCCTGCTGCCTCGGG
  ATGCCCAGGCTGAAGTTCATATGACTAATGCCGGCGCTCAGCTTGCTGGCGGGGCTCGGCACTCTTTCAG
  GTACAAGGGTAGAACCGAACCTTACCCTTCCCCTGCTATTAAGCGCGTGCTGATACGGGGGAAAGGCATC
  CAACTCAACGACGAGGTTACTTCTCCACTGGGCGTCCGGCCCGACGGAGTCTTCCAGCTGGGAGGATCTG
  GCAGAAGCTCCTTTACGGCACGGCAGGCATATCTCACCCTCCAGTCAAGCAGCTCCGCCCCTCGGTCCGG
  TGGTATCGGAACCCTCCAGTTCGTAGAAGAGTTCACGCCTTCCGTTTATTTCAATCCATTCAGCGGTTCA
  CCTGGCCACTATCCGGACGCCTTTATCCCTAACTTTGACGCGGTGTCAGAAAGCGTGGATGGGTACGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100272 representing NP_040928
  Red=Cloning sites Green=Tags
  
  MSKDIPTPYMWSFQPQMGLAAGAAQDYSSKMNWLSAGPHMISRVNGVRARRNQILLEQAALTATPRNQLN
  PPSWPAALIYQENPPPTTVLLPRDAQAEVHMTNAGAQLAGGARHSFRYKGRTEPYPSPAIKRVLIRGKGI
  QLNDEVTSPLGVRPDGVFQLGGSGRSSFTARQAYLTLQSSSSAPRSGGIGTLQFVEEFTPSVYFNPFSGS
  PGHYPDAFIPNFDAVSESVDGYD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 699 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040928
• RefSeq ORF: 699
• Locus ID: 1460842
• MW: 25.3 kDa