E2B (NC_001460) Virus Tagged ORF Clone

CAT#: VC100259

Myc-DDK-tagged ORF clone of viral ORF for E2B gene product [Human adenovirus A], codon optimized for human cell expression, NP_040915

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100259 represents NCBI reference of NP_040915 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGGTTCAAAATAATCGGGCAGGCTCCGTATACACTACTCCAACCGACAGTAGGTACCAGAGCC
  CTGGCTCCAGACCTTGTCAAAGTACCTCTGGCAGCACACCGTCACCAGCTAGAGCAAGCGGCAGACATAC
  AGCCAGCCCTGCATCCCGCAGGACAAGGACCGCTGCTTCTAGCGGCTCCTCCTCTGCTCCACCGATTCTC
  TCAAGGCGGATCGGTAAAGTGCATCGAGGGACCGTGGTTTCACCCCGGGCTCACGGCATCCTCCATGCGA
  TCGATAGCGCCAACGGCACCCCACTGGAGATTAAATATTACCTGCACCTGCAGCCTGCATTGACTCGGCT
  CTGCGAGGTGAACCTGCGAGCAGTACCCGCAGATATGCTGTTTTCACTGACTGATACCATGGATTCCAGC
  AGGCTGTACGCCCTCATCAGCCGCTTCCGACCCTCCAGGGCAGAAATATGGACTTGTCGGTCACGGGGAA
  CTGTTACCCTCAGCGTCTTGGCCTTCGAGGACCCCAACGGAAGCGGGAATGCGGCTGAGGAGGAGGAGGA
  TGAACGGCAACTCCCCTCAGGCATCGACTTCCCTATCTGTTTTCTGGTTAGGGGCAGACAGGTTCACCTC
  ATCCAGCAAATCCAGCCAGTGCAGCGCTGTGAACATTGCGCTAGGTTCTACAAACACCAGCACGAGTGCT
  CCGTACGGAGGAGGGACTTTTATTTTCACCACATTAACTCCCACAGCTCAAATTGGTGGCAGGAGATCCA
  TTTCTTCCCGATCGGATCTCACCCGCGCACAGAAAGGCTCTTCATCACATATGATGTCGAAACTTACACC
  TGGATGGGAGCTTTCGGGAAACAGCTGGTTCCGTTCATGCTGGTCATGAAACTCTCCGGTGATGACAATC
  TCGTGAAGCACGCCCTGCAGCTCGCTCTTGAACTCGGCTGGGATCAGTGGGAGAAGGATAGCACTACGTT
  TTATTGCCTCACCCCAGAAAAAATGAAGGTGGGCCAACAATTCAGGACTTATAGAAATAGACTGCAGACT
  AGCCTGGCAACTGATCTGTGGATGACTTTCCTTCAGAAGAACCCTCATCTTAGCCAGTGGGCACAGGAAG
  AAAATGGCCTCGTGGCCCTTGAAGACCTGTCATATGAAGATCTTAAGCGAGCCCCCGCGATTAAGGGGGA
  ACCACGGTTTGTGGAGCTGTACATCGTAGGCCACAACATTAATGGCTTTGACGAAATAGTTCTCGCAGCG
  CAGGTTATCAATAACCGACTTGATGTGCCTGGACCATTTAAGATTTCCAGAAATTTCATCCCTCGGGCCG
  GCAAAATCCTGTTTAATGACATCACCTTCGCCCTGCCTAATCCTCACTATAAAAAAAGAACAAATTTCCT
  TCTCTGGGAGCATGGGGGCTGTGATGATCAGGACTTCAAGTACCAGTACCTGAAGGTAATGGTTAGGGAT
  ACTTTCGCGTTGACACACACTTCACTTCGCAAGGCTGCTCAAGCATACGCACTGCCTGTGGAGAAAGGGT
  GTTGTCCTTACAAAGCAGTTAACCAGTTTTACATGCTGGGATCCTATCGGGCCGATGCCAATGGGTTCCC
  GCTGGAGGAATACTGGAAGGATAAGGAAGAGTATCTTCTTAACCAGGAACTCTGGAAGAAAAAGGGTGAA
  AAAAATTACAACTTGATAGGCGAGACACTGAACTATTGCGCACTGGATGTGTTGGTGACCGCAAGCCTGG
  TCGAGAAGCTTCGGTCCAGCTACGCTCAGTTCGTGACGGACGCTGTGGGCCTGGACGCAGCACATTTCAA
  TGTGTTTCAGAGGCCTACTATCTCCTCCAACAGTCATGCAATATTTAGGCAGATACTCTACAGGGCCGAA
  AAACCCCAACGAACGCACCTCGGGCCTAACATCCTCGCGCCCAGTCACGAACTGTATGATTATGTAAGAG
  CTTCTATTCGGGGAGGACGGTGCTATCCAACTTATATCGGTGTGTTGAAAGAGCCGATCTACGTGTACGA
  CATTTGCGGAATGTATGCAAGTGCCCTGACTCACCCAATGCCCTGGGGTCCACCACTTAATCCATACGAG
  CGAGCGCTTGCAGTCCGGCAGTGGCAGGTTGCTCTCGAAAACTACACTTGCAAAATCGATTATTTCGATA
  AAAACCTCTGTCCCGGCATCTTTACCATCGATGCTGACCCACCTGATGAGAACCAACTGGATGTGCTCCC
  CCCATTCTGTTCCCGAAAGGGCGGCCGCCTTGCCTGGACCAACGAGTCTTTGAGAGGGGAAGTCGTGACC
  TCAGTGGACCTGGTGACCCTCCACAATCGAGGATGGAGGTTGAGGCTCTTGTCCGATGAAAGAACAACAA
  TATTCCCTACCTGGAAATGCCTTGCGAGAGAGTATGTTCAGCTGAATATTGCTGCCAAGGAGAGAGCAGA
  TAGAGACAAGAACCAGACGTTGCGGTCTATAGCAAAACTTCTCTCAAACGCCCTGTACGGTTCTTTCGCC
  ACTAAGCTGGACAATAAAAAGATCGTTTTTTCAGACCAGATGGAGGAATCCCTGATGAAGGAAATCGCTG
  CGGGGAGGCTGAATATTAAGTCTTCTTCCTTTATCGAGACTGATACCCTGAGCACCGAGGTCATGCCCGC
  GTTTGAACGAGTGTACAGCCCAAACCAGTTGGCCCTGGTGAACTCCGAGGCAGAGGAGTCTGATGAAGAT
  CAAGGTCCTGCACCTTTTTACAGCCCTCCTCCCGAAAATTGTGAGCATGTGACTTACACTTACAAGCCCA
  TCACCTTCATGGATGCGGAGGAGGGGGACATGTGCCTTCACACTTTGGAATCCTCCAACCCACTTATCAA
  TAATGACAGGTATCCCAGCCACGTCGCTTCATTCGTTCTCGCTTGGACTAGGGCATTCGTTAGTGAGTGG
  TCCGAGTTCCTCTATGAAGAAGATAGAGGCATACCACTGAAAGACCGCCCTCTTAAGAGCGTTTATGGCG
  ACACTGACTCCCTCTTTGTTACTGAAAAAGGCAGGCGCTTGATGGAGAGCCAAGGCAAGAAACGCATAAA
  GAAGTACGGAGGTAAGCTCGTGTTTGATCCTTCTTGCCCGGAGTTGACCTGGTCAGTGGAATGTGAAACC
  GTGTGTAGTTATTGCGGGGCCGACGCATACTCCCCTGAAAGTGTGTTCCTGGCCCCTAAACTCTATGCAT
  TGAAGTGCCTGCAGTGTCCTCACTGCGGCAGCACGTCTAAAGGGAAGATTAGGGCCAAGGGTCATGCAAC
  CGAAGCTCTCTCCTATGATTTGATGCTGAAGTGCTACCTGGCCGAGGCCCAGGGAGAAGATACACGATTT
  TCCACCTCTCGGCTGTCACTGAAGAGAACTTTGGCAAGTGCCCAGCCCGGCGCGCATCCATTCACTGTCA
  CAGAAACAACCCTGACCCGCACTCTTAGGCCCTGGAAGGACAAGACCCTGGTCCATCTGGATGCCCACCG
  CCTCGTGCCCTATTCTAATTCTCAACCTAACCCGAGGAACGAAGAGGTCTGCTGGATCGAGATGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100259 representing NP_040915
  Red=Cloning sites Green=Tags
  
  MALVQNNRAGSVYTTPTDSRYQSPGSRPCQSTSGSTPSPARASGRHTASPASRRTRTAASSGSSSAPPIL
  SRRIGKVHRGTVVSPRAHGILHAIDSANGTPLEIKYYLHLQPALTRLCEVNLRAVPADMLFSLTDTMDSS
  RLYALISRFRPSRAEIWTCRSRGTVTLSVLAFEDPNGSGNAAEEEEDERQLPSGIDFPICFLVRGRQVHL
  IQQIQPVQRCEHCARFYKHQHECSVRRRDFYFHHINSHSSNWWQEIHFFPIGSHPRTERLFITYDVETYT
  WMGAFGKQLVPFMLVMKLSGDDNLVKHALQLALELGWDQWEKDSTTFYCLTPEKMKVGQQFRTYRNRLQT
  SLATDLWMTFLQKNPHLSQWAQEENGLVALEDLSYEDLKRAPAIKGEPRFVELYIVGHNINGFDEIVLAA
  QVINNRLDVPGPFKISRNFIPRAGKILFNDITFALPNPHYKKRTNFLLWEHGGCDDQDFKYQYLKVMVRD
  TFALTHTSLRKAAQAYALPVEKGCCPYKAVNQFYMLGSYRADANGFPLEEYWKDKEEYLLNQELWKKKGE
  KNYNLIGETLNYCALDVLVTASLVEKLRSSYAQFVTDAVGLDAAHFNVFQRPTISSNSHAIFRQILYRAE
  KPQRTHLGPNILAPSHELYDYVRASIRGGRCYPTYIGVLKEPIYVYDICGMYASALTHPMPWGPPLNPYE
  RALAVRQWQVALENYTCKIDYFDKNLCPGIFTIDADPPDENQLDVLPPFCSRKGGRLAWTNESLRGEVVT
  SVDLVTLHNRGWRLRLLSDERTTIFPTWKCLAREYVQLNIAAKERADRDKNQTLRSIAKLLSNALYGSFA
  TKLDNKKIVFSDQMEESLMKEIAAGRLNIKSSSFIETDTLSTEVMPAFERVYSPNQLALVNSEAEESDED
  QGPAPFYSPPPENCEHVTYTYKPITFMDAEEGDMCLHTLESSNPLINNDRYPSHVASFVLAWTRAFVSEW
  SEFLYEEDRGIPLKDRPLKSVYGDTDSLFVTEKGRRLMESQGKKRIKKYGGKLVFDPSCPELTWSVECET
  VCSYCGADAYSPESVFLAPKLYALKCLQCPHCGSTSKGKIRAKGHATEALSYDLMLKCYLAEAQGEDTRF
  STSRLSLKRTLASAQPGAHPFTVTETTLTRTLRPWKDKTLVHLDAHRLVPYSNSQPNPRNEEVCWIEMA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 3567 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040915
• RefSeq ORF: 3567
• Locus ID: 1460849
• MW: 135.2 kDa