L3 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100268

Myc-DDK-tagged ORF clone of viral ORF for L3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040924

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100268 represents NCBI reference of NP_040924 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACTCCAAGCATGATGCCACAGTGGAGTTACATGCACATCGCCGGACAGGATGCGAGCGAGTATT
  TGAGTCCAGGTCTCGTTCAGTTCGCGAGAGCCACTGATACTTATTTTACCCTCGGGAACAAATTTAGGAA
  TCCAACAGTAGCTCCAACACACGACGTGACCACTGACCGCTCCCAGAGGCTTACCCTCCGGTTCGTGCCC
  GTCGATCGGGAAGATACAACATATTCCTATAAAGCCCGCTTCACTCTCGCCGTCGGAGACAACAGGGTAC
  TGGATATGGCTTCAAGCTACTTCGACATCAGGGGCGTGCTGGATAGAGGCCCGTCATTTAAACCTTACAG
  CGGTACAGCGTATAATTCTCTGGCCCCCAAGGGCGCTCCGAACGCATCTCAATGGAGTGATAACGCAAAG
  CTCAACACCTTCGCACAGGCTCCATACCTCAGTGATACCATCACAGCCGCCGATGGGATTAAGGTCGGTA
  CCGACACTGCCCAGGCCGGAGCCGCGGTATATGCTAATAAGACTTATCAACCAGAGCCACAGGTAGGCCC
  TTCTGAGTGGAACACGAGCATTGAGAACGTTAAGGCGGGCGGCCGAGCTTTGAAGCAAACAACCGCAATG
  CAACCTTGCTATGGTTCTTACGCACGCCCGACTAATGAACATGGCGGCCAGTCCAAAGACGATAATATTG
  AACTGAAGTTCTTTGACTCTGCCAACAACGCAGCGAACACTGCGCAGGTTGTTTTCTATACCGAAGATGT
  GAACCTCGAAATGCCGGATACGCACCTGGTGTTTAAACCAACCGTCACTAACGGGACCATCGCCAGTGAG
  TCTCTGTTGGGCCAGCAGGCTGCCCCAAACCGCGCAAACTACATCGCTTTCAGAGACAACTTTATCGGGC
  TTATGTACTACAATTCTACTGGGAACATGGGAGTGCTTGCTGGGCAGGCATCTCAGCTGAATGCAGTAGT
  GGATCTCCAAGATCGCAATACCGAGCTCTCTTATCAACTCATGCTGGATGCGCTGGGGGATCGGACCCGC
  TACTTTAGCCTGTGGAACAGCGCCGTGGATTCATACGACCCGGATGTCCGGGTGATCGAGAACCATGGAG
  TCGAAGACGAATTGCCCAACTACTGTTTTCCACTGAGCGCGGTGGGAGAGATTAAAAACTATAAGGGGAT
  CAAACCCGACAACGGCGGCGGTGGCGGGTGGACAGCCGATAACACAGTTAGCGAGGCCAACCATATTGGA
  ATTGGCAATATTGCCGCAATGGAGATTAACCTTCAGGCCAACCTGTGGCGCAGTTTCCTGTACTCCAACG
  TTGGCCTTTACCTGCCCGATGATCTGAAATACACGCCAGGTAACATAAAACTCCCCGATAACAAGAACAC
  GTACGAGTATATGAATGGCCGGGTAACAGCTCCCGGGTTGGTGGACACTTACGTGAATATAGGCGCCCGC
  TGGAGCCCTGACGTGATGGACAACGTTAATCCCTTCAATCATCATCGGAACGCGGGCCTGCGCTATCGGA
  GTATGCTGTTGGGCAATGGAAGGTTCGTCCCTTTTCATATCCAAGTGCCCCAGAAGTTTTTCGCAATTCG
  GAACCTGCTCCTCCTTCCAGGCAGTTACACATACGAGTGGAACTTCAGAAAGGATGTGAATATGATTCTT
  CAATCTACTCTGGGCAATGACCTGAGAGTGGACGGCGCCTCTGTGCGGTTTGATAACATCGCGCTGTATG
  CTAATTTTTTCCCTATGGCTCACAACACAGCCTCCACGTTGGAAGCTATGCTGCGCAACGATACCAACGA
  TCAGTCTTTCAACGATTATCTCTGCGCAGCAAACATGCTCTATCCAATCCCCGCCAACGCAACCTCCGTT
  CCGATCTCAATTCCCTCCCGGAACTGGGCAGCCTTTAGAGGCTGGAGTTTCACGCGACTTAAGACGAAAG
  AAACGCCTTCTCTTGGTTCCGGGTTCGATCCATACTTTGTGTATTCCGGTACAATCCCGTATCTGGACGG
  AACCTTCTACCTTAACCACACTTTCAAGAAAGTTTCAATCATGTTCGACTCATCCGTTTCCTGGCCGGGG
  AATGATCGGCTGCTGACTCCCAATGAATTTGAGATCAAACGCTCTGTCGACGGAGAAGGCTATAACGTGG
  CACAATGCAATATGACCAAAGACTGGTTCCTGATTCAGATGCTCTCTCATTATAACATCGGCTATCAGGG
  CTTCTATATACCAGAATCTTACAAAGATCGGATGTACTCATTTTTTCGGAACTTTCAGCCCATGAGTCGA
  CAGGTGGTCGATACCACAGAGTATAAGAACTATAAAAAGGTGACAGTGGAGTTCCAGCACAATAATAGCG
  GGTTCGTGGGATATCTGGGGCCAACGATGAGAGAGGGCCAGGCCTATCCCGCCAATTATCCTTATCCTCT
  GATAGGTCAGACCGCTGTTGAGTCCATCACCCAGAAAAAATTCCTGTGCGATAGGGTCATGTGGCGGATC
  CCTTTCTCATCAAACTTTATGTCTATGGGAGCTCTCACTGATCTCGGACAAAACATGCTCTATGCAAATT
  CCGCACACGCCCTTGATATGACCTTTGAGGTTGACCCTATGGATGAACCCACCCTTCTCTATGTTCTCTT
  CGAGGTCTTTGATGTCGTGCGGATTCACCAGCCACACCGCGGCGTCATAGAGGCTGTGTACCTGCGCACC
  CCATTCAGCGCTGGCAACGCTACAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100268 representing NP_040924
  Red=Cloning sites Green=Tags
  
  MATPSMMPQWSYMHIAGQDASEYLSPGLVQFARATDTYFTLGNKFRNPTVAPTHDVTTDRSQRLTLRFVP
  VDREDTTYSYKARFTLAVGDNRVLDMASSYFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNASQWSDNAK
  LNTFAQAPYLSDTITAADGIKVGTDTAQAGAAVYANKTYQPEPQVGPSEWNTSIENVKAGGRALKQTTAM
  QPCYGSYARPTNEHGGQSKDDNIELKFFDSANNAANTAQVVFYTEDVNLEMPDTHLVFKPTVTNGTIASE
  SLLGQQAAPNRANYIAFRDNFIGLMYYNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLMLDALGDRTR
  YFSLWNSAVDSYDPDVRVIENHGVEDELPNYCFPLSAVGEIKNYKGIKPDNGGGGGWTADNTVSEANHIG
  IGNIAAMEINLQANLWRSFLYSNVGLYLPDDLKYTPGNIKLPDNKNTYEYMNGRVTAPGLVDTYVNIGAR
  WSPDVMDNVNPFNHHRNAGLRYRSMLLGNGRFVPFHIQVPQKFFAIRNLLLLPGSYTYEWNFRKDVNMIL
  QSTLGNDLRVDGASVRFDNIALYANFFPMAHNTASTLEAMLRNDTNDQSFNDYLCAANMLYPIPANATSV
  PISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGTIPYLDGTFYLNHTFKKVSIMFDSSVSWPG
  NDRLLTPNEFEIKRSVDGEGYNVAQCNMTKDWFLIQMLSHYNIGYQGFYIPESYKDRMYSFFRNFQPMSR
  QVVDTTEYKNYKKVTVEFQHNNSGFVGYLGPTMREGQAYPANYPYPLIGQTAVESITQKKFLCDRVMWRI
  PFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDPMDEPTLLYVLFEVFDVVRIHQPHRGVIEAVYLRT
  PFSAGNATT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040924
• RefSeq ORF: 2757
• Locus ID: 1460858
• MW: 103.0 kDa