E1B (NC_003266) Virus Tagged ORF Clone

CAT#: VC100463

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 19K [Human adenovirus E], codon optimized for human cell expression, YP_068019

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100463 represents NCBI reference of YP_068019 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATTTGGACTGTGCTCGAAGACTTCCACAAGACAAGGCAGCTGCTGGAGAACGCAAGTAATGGCG
  TATCATACCTGTGGCGATTCTGTTTTGGCGGAGACCTGGCTAAACTGGTTTACAGAGCCAAGCAGGACTA
  TAGAGAGCAGTTCGAAGACATACTTAGGGAGTGCCCTGGGCTGTTCGACGCCCTCAACCTCGGCCACCAG
  TCCCATTTCAACCAGCGCATCAGCAGAGCGCTGGACTTCACCACCCCAGGGAGGACGACAGCCGCTGTGG
  CCTTTTTTGCGTTCATTTTCGATAAGTGGTCTCAGGAGACTCATTTTTCCCGCGATTATCAACTCGATTT
  TCTGGCCGTAGCTTTGTGGCGCACTTGGAAGTGTCAGCGCCTGAACGCCATCTCCGGGTATCTGCCCGTT
  CAGCCACTGGATACTCTGAGAATACTGTCCCTTCAGCAGCAGGAAGATCAGGAAGAGAATCCGCGGGCAG
  GGTTGGATCCGCCCGCCGAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100463 representing YP_068019
  Red=Cloning sites Green=Tags
  
  MEIWTVLEDFHKTRQLLENASNGVSYLWRFCFGGDLAKLVYRAKQDYREQFEDILRECPGLFDALNLGHQ
  SHFNQRISRALDFTTPGRTTAAVAFFAFIFDKWSQETHFSRDYQLDFLAVALWRTWKCQRLNAISGYLPV
  QPLDTLRILSLQQQEDQEENPRAGLDPPAEE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 513 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068019
• RefSeq ORF: 513
• Locus ID: 2958470
• MW: 20.0 kDa