L5 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100492

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus E], codon optimized for human cell expression, YP_068048

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100492 represents NCBI reference of YP_068048 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAGAAGAGGGCACGGGTGGATGATGGCTTTGATCCCGTCTATCCCTATGACGCTGATAACGCAC
  CAACAGTGCCGTTTATTAACCCTCCTTTCGTTTCAAGTGATGGATTCCAGGAGAAACCACTCGGAGTACT
  GTCACTGCGCCTGGCAGACCCTGTCACCACAAAGAACGGCGAGATCACCCTGAAGCTTGGGGAAGGGGTG
  GACCTGGACGATAGCGGAAAACTTATCGCAAATACAGTGAACAAGGCCATAGCTCCACTGAGCTTCTCAA
  ACAACACAATATCCCTCAACATGGACACACCCCTGTACACGAAGGACGGAAAGCTCTCCCTCCAAGTCTC
  TCCTCCCTTGTCTATCCTGAAGTCCACCATACTTAACACCCTGGCTCTGGCTTTCGGTTCAGGACTGGGC
  TTGTCAGGCAGTGCCCTCGCTGTTCAGTTGGCCAGCCCTCTCACTTTCGATGACAAAGGTAACATTAAAA
  TTACTCTGAACCGGGGCCTGCATGTGACAACCGGAGACGCCATCGAGTCTAATATCAGCTGGGCCAAGGG
  CATTAAGTTCGAAGACGGGGCCATTGCTACAAACATCGGGAAGGGACTGGAATTCGGCACAAGTTCTACT
  GAAACCGGGGTGAATAACGCTTATCCAATCCAGGTGAAACTGGGATCAGGACTCAGTTTCGACTCAACTG
  GTGCAATCATGGCCGGAAATAAAGACTACGACAAACTCACCCTGTGGACTACCCCGGACCCCAGCCCTAA
  CTGCCAGATTCTTGCGGAGAACGACGCTAAGCTGACACTCTGCCTGACCAAATGTGATTCCCAGATTTTG
  GCTACCGTTTCTGTGCTCGTGGTGCGCAGCGGAAATCTCAATCCCATAACAGGGACTGTGTCATCCGCTC
  AGGTTTTTCTCAGATTTGACGCCAATGGCGTACTCCTCACTGAACACTCCACGCTGAAGAAGTACTGGGG
  TTACAAGCAGGGAGACTCTATCGACGGAACCCCTTACACAAACGCAGTGGGCTTCATGCCTAACTCAACT
  GCCTACCCAAAGACCCAGTCCTCCACCACGAAGAACAATATAGTGGGCCAGGTGTACATGAATGGCGACG
  TGTCCAAACCAATGCTCTTGACGATAACCCTGAATGGGACTGATGATACAACAAGTGCCTACTCCATGAG
  CTTCTCCTACACGTGGACGAATGGCTCTTACATAGGCGCAACGTTTGGCGCCAACTCTTACACTTTCAGC
  TACATCGCACAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100492 representing YP_068048
  Red=Cloning sites Green=Tags
  
  MSKKRARVDDGFDPVYPYDADNAPTVPFINPPFVSSDGFQEKPLGVLSLRLADPVTTKNGEITLKLGEGV
  DLDDSGKLIANTVNKAIAPLSFSNNTISLNMDTPLYTKDGKLSLQVSPPLSILKSTILNTLALAFGSGLG
  LSGSALAVQLASPLTFDDKGNIKITLNRGLHVTTGDAIESNISWAKGIKFEDGAIATNIGKGLEFGTSST
  ETGVNNAYPIQVKLGSGLSFDSTGAIMAGNKDYDKLTLWTTPDPSPNCQILAENDAKLTLCLTKCDSQIL
  ATVSVLVVRSGNLNPITGTVSSAQVFLRFDANGVLLTEHSTLKKYWGYKQGDSIDGTPYTNAVGFMPNST
  AYPKTQSSTTKNNIVGQVYMNGDVSKPMLLTITLNGTDDTTSAYSMSFSYTWTNGSYIGATFGANSYTFS
  YIAQQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068048
• RefSeq ORF: 1275
• Locus ID: 2958476
• MW: 45.2 kDa