E2B (NC_003266) Virus Tagged ORF Clone

CAT#: VC100468

Myc-DDK-tagged ORF clone of viral ORF for terminal protein precursor pTP [Human adenovirus E], codon optimized for human cell expression, YP_068024

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100468 represents NCBI reference of YP_068024 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTTTCAATAAACGACTGCGCACGGCTCACCGGCCAGACAGTCCCCACCATGAACTACTTCTTGC
  CTTTGAGGAACATTTGGAATCGCGTTCGGGAGTTTCCGCGCGCCAGCACCACAGCCGCCGGAATCACGTG
  GATGAGCCGGTACATCTATGGCTATCATAGGACTATGCTGGAAGACCTGGCACCGGGCGCACCAGCAACC
  GAGCGGTGGCCACTCTATCGGCAGCCCCCACCCCATTTCCTGATAGGATATCAGTACCTTGTACGCACCT
  GCAACGACTACATTTTTGATAGCCGGGCTTACTCAAGACTGAAGTACCACGAGCTGGCTCGCCCTGGGCA
  TCAGACCGTCAATTGGTCAGTCATGGCTAATTGCTCATATACTATCAATACTGGAGCTTACCATAGATTT
  GTAGACTTTGACGACTTCCAGACAACGTTGACACAGATTCAGCAGGCTATTCTGGCTGAGCGCGTGGTGG
  CTGATCTGGCTTTGGTTCAGCCACAAAGAGGTTTCGGCCTGACGCGAATGCACGGGCGGGCAGGAGAGGA
  AGAGGTGCCTGTAGAGCGACTCATGCAAGATTACTATAAGGATCTGGCCCGGTGTCAGGACCATGCATGG
  GGCATGGCCGACAGGCTGCGGATACAGCAGGCCGGCCCTAAGGACCTGGTGCTCTTGGCAACAATCCGCA
  GACTCAGGACTGCTTACTTTAATTTTATCACCAGCAGTATTGCCCCCGCCCATACTCCACCCCCCCCTGA
  GGAAACCGTTCTGAGCTTGCCTTGCGATTGTGATTGGCTGGAAGCCTTTGTGCAGAGGTTCTCAGATCCA
  GTCGATCTGGAAACTCTGAGGTCCTTGAGAGGCGTCCCGACTGGGCAGTTGATCAGGTGCATCGTATCTG
  CACTGTCTTTGCCAAATGGCGATCCACCAGGTCACCTGGAAATGCGCGGGGGGGTCTTTACTTTGAGACC
  GCGAGAGGATGGGAGGGCCGTGACTGAGACAATGCGGCGAAGGAGGGGCGAAACCATCGAAAGATTCATC
  GATAGGTTGCCCGTCCGGAGACGAAGACGGAGGCCACCCCCACCCCCTCCACCCCCAGAGGAGGAGGTCG
  AAGAAATGCTGGTTGATGAGGAGGAGGAAGTCGAGGAATTGCCCGGAGCTTTCGAAAGGGAAGTCCGCGC
  TACGATAGCAGAACTGATCAGGCTCTTGGAGGAAGATCTGACAGTCAGTGCCAGAAATTCACAGTTTTTT
  AATTTCGCGGTAGATTTCTACGAAGCAATGGAGAGGCTGGAAGCACTGGGGGACGTAAGTGAGATGCCCC
  TCCGGCGATGGATAATGTATTTCTTCGTCACGGAACACATCGCCACCACCCTCAATTATCTCTATCAGAG
  ATTGTGCAATTACGCGGTTTTTGCTCGCCATGTCGAACTCAATCTCGCTCAGGTAGTGATGCGCGCAAGG
  GACCCTGAAGGGGCGGTGGTGTACTCCAGGGTGTGGAATGAAGCGGGGATGAACGCCTTCAGCCAGCTGA
  TGGGCCGAATCAGCAATGACCTTGCAGCCACCGTAGAAAGGGCTGGCAGAGGTGACCTCCAAGAGGAGGA
  GATCGAGCAATTCATGACAGAAATTGCCTATCAGGATAACAGTGGCGACGTCCAAGAAATTCTGCGGCAG
  GCTGCTGTTAATGACACTGAGATTGATTCTGTGGAGTTGAGTTTCAGATTCAAGCTGACAGGCCCTGTGG
  CTTTCACTCAAAGAAGGCAGATTCAGGATGTTAATAGAAGAGTAGTCGCCCATGCCAGCCTTCTCAGGGC
  ACAGTATCAAAACCTGCCGGCTAGAGGGGCCGATGTGCCGCTTCCACCGCTGCCTCCTGGCCCAGAGCCA
  CCCCTCCCTCCGGGTGCAAGACCCAGGCGCCGCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100468 representing YP_068024
  Red=Cloning sites Green=Tags
  
  MALSINDCARLTGQTVPTMNYFLPLRNIWNRVREFPRASTTAAGITWMSRYIYGYHRTMLEDLAPGAPAT
  ERWPLYRQPPPHFLIGYQYLVRTCNDYIFDSRAYSRLKYHELARPGHQTVNWSVMANCSYTINTGAYHRF
  VDFDDFQTTLTQIQQAILAERVVADLALVQPQRGFGLTRMHGRAGEEEVPVERLMQDYYKDLARCQDHAW
  GMADRLRIQQAGPKDLVLLATIRRLRTAYFNFITSSIAPAHTPPPPEETVLSLPCDCDWLEAFVQRFSDP
  VDLETLRSLRGVPTGQLIRCIVSALSLPNGDPPGHLEMRGGVFTLRPREDGRAVTETMRRRRGETIERFI
  DRLPVRRRRRRPPPPPPPPEEEVEEMLVDEEEEVEELPGAFEREVRATIAELIRLLEEDLTVSARNSQFF
  NFAVDFYEAMERLEALGDVSEMPLRRWIMYFFVTEHIATTLNYLYQRLCNYAVFARHVELNLAQVVMRAR
  DPEGAVVYSRVWNEAGMNAFSQLMGRISNDLAATVERAGRGDLQEEEIEQFMTEIAYQDNSGDVQEILRQ
  AAVNDTEIDSVELSFRFKLTGPVAFTQRRQIQDVNRRVVAHASLLRAQYQNLPARGADVPLPPLPPGPEP
  PLPPGARPRRRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068024
• RefSeq ORF: 1926
• Locus ID: 2958478
• MW: 73.7 kDa