L3 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100475

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVI [Human adenovirus E], codon optimized for human cell expression, YP_068031

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100475 represents NCBI reference of YP_068031 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATATTAACTTCTCATCCTTGGCCCCGCGGCATGGGACGAGGCCTTTTATGGGAACCTGGTCAG
  ATATAGGAACGTCTCAGCTCAATGGGGGGGCGTTTAATTGGTCCAGCCTGTGGAGCGGGCTCAAAAATTT
  TGGCTCTACCCTGAAAACCTACGGTTCTAAGGCGTGGAACAGTACTACAGGACAAGCACTTAGGGATAAG
  CTGAAGGAGCAAAATTTTCAGCAGAAAGTAGTTGACGGCTTGGCATCTGGAATCAATGGTGTGGTGGATT
  TGGCTAATCAGGCTGTGCAGAGACAGATCAATAGCAGGCTGGATCCCGTGCCACCCGCAGGATCTGTTGA
  GATGCCGCAGGTGGAGGAGGAACTGCCACCGCTCGACAAAAGGGGGGACAAGCGCCCCCGCCCGGATGCT
  GAGGAAACACTGCTCACACACACCGACGAACCTCCACCATATGAAGAAGCAGTAAAACTGGGACTGCCTA
  CCACGAGACCAATTGCCCCCCTCGCCACCGGCGTTCTCAAGCCCGAATCATCCAAGCCTGCAACCCTGGA
  CCTGCCTCCTCCTCCAACGCCAAGACCATCTACAGTGGCAAAGCCCCTCCCCCCAGTGGCTGTTGCCCGC
  GCTCGGCCAGGAGCCCGACCCCAGGCCAATTGGCAGTCTACCCTGAACAGCATCGTTGGGCTCGGCGTAC
  AAAGCGTGAAGAGGAGGAGATGTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100475 representing YP_068031
  Red=Cloning sites Green=Tags
  
  MEDINFSSLAPRHGTRPFMGTWSDIGTSQLNGGAFNWSSLWSGLKNFGSTLKTYGSKAWNSTTGQALRDK
  LKEQNFQQKVVDGLASGINGVVDLANQAVQRQINSRLDPVPPAGSVEMPQVEEELPPLDKRGDKRPRPDA
  EETLLTHTDEPPPYEEAVKLGLPTTRPIAPLATGVLKPESSKPATLDLPPPPTPRPSTVAKPLPPVAVAR
  ARPGARPQANWQSTLNSIVGLGVQSVKRRRCY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 726 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068031
• RefSeq ORF: 726
• Locus ID: 2958473
• MW: 26.2 kDa