E2A (NC_003266) Virus Tagged ORF Clone

CAT#: VC100478

Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human adenovirus E], codon optimized for human cell expression, YP_068034

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100478 represents NCBI reference of YP_068034 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGACGGGGAGGGTCTCAACTTGAGCGGCGGAGGGAGAGGACCCCAGACCGGGGTCGCGGAAGTG
  CATCCCATCCTCCCGGACGGGAATCTCCGTCACCACCTCCACTGCCGCTGAAACGCCATACTTACCGCCG
  CGTCGCCAGCGATCAGGAAGAGGAGATAGTGGTGGTTAGTGAAAACTCTCGGTCACCTAGCCCCCAGGAG
  CAGCCCCCCCCACCCCAGCAGCCTCCTAAAAAGAAGCCGAGGAAGACAAAACATGTGCCTCTTCAGGACG
  TAAGCCAGGACTCCGAGGACGAAAGAGAGGCCGAAGAGGAGCTCGCAGCAGTGGGCTTCTCCTACCCCCC
  CGTGAGGATTACCGAGAAAGACGGAAAGAGATCTTTTGAGACCCTGAATGAGAATGATCCCCTTACAAAA
  GCAGCATCTGCCAAGATGGCTGTGACTAATCCCCTCTCCCTCCCAATAGTGAGCGCATGGGAGAAAGGAA
  TGGAGATAATGAATATGCTGATGGAAAGATACCGCGTTGAGTCTGATCTGAAATCAAACTTTCAGCTGAT
  GCCGGAACAGGGAGAAGTTTACAGGAGAATCTGCCATCTCTATATCAACGAAGAACACAGGGGAATCCCC
  TTGACCTTCACATCCAACAAAACCCTCACCACAATGATGGGGCGGTTTTTGCAGGGCTTTGTCCACGCTC
  ATAGCCAGATCGCCCACAAGAATTGGGAGAGCACCGGCTGTGCGCTCTGGCTCCATGGGTGCACCGAAGT
  CGAGGGGAAACTTCGGTGTCTCCATGGCACAACAATGATCCAGAAAGAGCATATGATCGAGATGGACGTG
  GCAAGCGAGAATGGACAGCGAGCCCTGAAAGAGAATCCAGACAGAGCCAAGATCACACAAAACCGCTGGG
  GCAGATCTGTGGTTCAGCTTGCAAACAACGACGCTCGCTGCTGCGTCCACGATGCTGGGTGTGCAACCAA
  TCAGTTCTCCAGTAAGTCTTGTGGTGTGTTTTTTACAGAAGGCGCGAAAGCCCAGCAGGCGTTTAAGCAG
  CTGGAGGCCTTCATGAAGGCTATGTACCCTGGGATGAATGCGGACCAAGCCCAAATGATGCTGATCCCCC
  TGCACTGTGACTGTAACCATAAGCCAGGGTGTGTGCCGACTATGGGGCGCCAGACGTGTAAGATGACCCC
  GTTTGGAATGGCCAACGCCGAAGACCTGGATGTTGACGGAATCACAGATGCTACAGTTCTCGCTTCCGTG
  AAACACCCGGCCCTGATGGTTTTTCAGTGCTGCAACCCAGTTTACCGAAACAGTCGAGCACAAAACGCAG
  GGCCAAACTGTGACTTCAAAATCAGCGCTCCAGATCTGCTGGGGGCACTCCAGCTCACACGAAAGCTTTG
  GACAGACTCCTTTCCGGACACCCTGTTGCCTAAGCTGCTGATCCCTGAATTTAAGTGGCTTGCAAAGTAT
  CAGTTCCGAAATGTCTCCCTGCCAGCGGGGCACGCAGAGACATCCCGCCAGAATCCCTTTGATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100478 representing YP_068034
  Red=Cloning sites Green=Tags
  
  MAGRGGSQLERRRERTPDRGRGSASHPPGRESPSPPPLPLKRHTYRRVASDQEEEIVVVSENSRSPSPQE
  QPPPPQQPPKKKPRKTKHVPLQDVSQDSEDEREAEEELAAVGFSYPPVRITEKDGKRSFETLNENDPLTK
  AASAKMAVTNPLSLPIVSAWEKGMEIMNMLMERYRVESDLKSNFQLMPEQGEVYRRICHLYINEEHRGIP
  LTFTSNKTLTTMMGRFLQGFVHAHSQIAHKNWESTGCALWLHGCTEVEGKLRCLHGTTMIQKEHMIEMDV
  ASENGQRALKENPDRAKITQNRWGRSVVQLANNDARCCVHDAGCATNQFSSKSCGVFFTEGAKAQQAFKQ
  LEAFMKAMYPGMNADQAQMMLIPLHCDCNHKPGCVPTMGRQTCKMTPFGMANAEDLDVDGITDATVLASV
  KHPALMVFQCCNPVYRNSRAQNAGPNCDFKISAPDLLGALQLTRKLWTDSFPDTLLPKLLIPEFKWLAKY
  QFRNVSLPAGHAETSRQNPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068034
• RefSeq ORF: 1536
• Locus ID: 2958469
• MW: 57.5 kDa