L4 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100482

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVIII [Human adenovirus E], codon optimized for human cell expression, YP_068038

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100482 represents NCBI reference of YP_068038 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAAGAGATCCCGACACCCTACATGTGGAGCTACCAGCCGCAAATGGGCCTGGCGGCTGGCGCGG
  CGCAGGACTACTCTACTAGAATGAATTGGCTCTCTGCTGGACCCGGGATGATCTCAAGGGTGAACGATAT
  CCGCGCGCACAGAAACCAAATACTGCTCGAGCAGTCCGCTCTGACCGCAACACCCAGAAACCATTTGAAT
  CCGAGGAATTGGCCCGCCGCCCTTGTTTACCAGGAGATCCCTCAGCCGACTACCGTGCTCCTCCCACGAG
  ACGCTCAGGCCGAGGTCCAACTTACCAATTCTGGCGTACAACTTGCCGGAGGAGCTACTCTGTGTAGGCA
  TCATCCAGCACAGGGAATCAAACGCCTGGTTATCCGGGGGAGGGGTACCCAGCTGAACGATGAAGTTGTT
  AGCTCAAGCTTGGGACTGCGGCCCGACGGCGTGTTTCAACTGGCCGGAAGCGGTCGATCCTCTTTTACCC
  CGCGGCAGGCAGTGCTGACCCTTGAGTCTAGCTCATCTCAACCCCGGAGCGGAGGAATCGGCACACTGCA
  GTTTGTCGAAGAGTTCACTCCTTCTGTCTATTTTAATCCATTTAGTGGCAGCCCAGGCCATTATCCAGAC
  GAGTTCATCCCTAATTTCGATGCAATTAGCGAAAGCGTTGACGGGTACGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100482 representing YP_068038
  Red=Cloning sites Green=Tags
  
  MSKEIPTPYMWSYQPQMGLAAGAAQDYSTRMNWLSAGPGMISRVNDIRAHRNQILLEQSALTATPRNHLN
  PRNWPAALVYQEIPQPTTVLLPRDAQAEVQLTNSGVQLAGGATLCRHHPAQGIKRLVIRGRGTQLNDEVV
  SSSLGLRPDGVFQLAGSGRSSFTPRQAVLTLESSSSQPRSGGIGTLQFVEEFTPSVYFNPFSGSPGHYPD
  EFIPNFDAISESVDGYD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068038
• RefSeq ORF: 681
• Locus ID: 2958479
• MW: 24.7 kDa