IVa2 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100466

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein IVa2 [Human adenovirus E], codon optimized for human cell expression, YP_068022

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100466 represents NCBI reference of YP_068022 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTCGCGGTCGGCGCTCCGGCGCTGTGCTCGACCAGCCTGATGAGCCGGAAGCCCACCCCCGGA
  AACGGCCTGCAAGACGAGCACCACTGTATCGCGATGGAGACCACCCCGATGCAGATGCCGCTACACTGGA
  AGGCCCCGACCCCGGTAGGGCAGGACGGCCATCATCAGGCGCACTGCTTCCTCAGTCTCCGCAGCCAGCA
  AAAAGGGGAGGCCTGCTTGATCGAGATGCCGTGGAACATATTACCGAGCTCTGGGACAGGCTTGAACTTC
  TCCAGCAGACCCTGAGTAAGATGCCCATGGCCGACGGCTTGAAACCATTGAAAAACTTCGCCTCTCTCCA
  GGAACTCCTCTCACTGGGGGGAGAGAGACTTCTGGCAGAACTGGTGCGGGAAAACATGCATGTGCGACAA
  ATGATGAACGAAGTGGCACCCCTCCTGCGCGAGGATGGCTCTTGTGTTAGCCTGAATTACCACCTGCAGC
  CCGTCATTGGCGTGATCTACGGGCCGACAGGTTGTGGCAAATCTCAGCTTCTGCGGAACCTGTTGAGCGC
  CCAGTTGATCAGCCCAGCCCCCGAGACTGTTTTTTTCATAGCCCCTCAGGTGGATATGATTCCCCCCAGT
  GAGCTTAAGGCATGGGAGATGCAGATCTGTGAAGGAAACTACGCACCTGGGATCGAGGGGACATTTGTCC
  CCCAGTCAGGAACGCTGCGGCCCAAGTTCATTAAGATGGCGTACGAAGACCTCACTCAAGACCACAACTA
  CGATGTCTCAGACCCGAGAAACGTGTTCGCCCAGGCAGCCGCGCATGGACCAATCGCCATAATAATGGAC
  GAGTGTATGGAAAATCTGGGAGGGCACAAGGGGGTCTCTAAGTTCTTCCACGCTTTTCCTTCAAAATTGC
  ACGATAAATTTCCTAAGTGTACCGGCTACACGGTGCTTGTGGTGCTCCATAATATGAATCCCCGACGGGA
  CCTGGGCGGGAACATTGCAAACTTGAAAATACAGGCCAAGATGCACCTTATTTCCCCCCGCATGCACCCG
  AGTCAGCTTAACCGGTTCGTCAATACTTACACCAAAGGGCTGCCCGTCGCTATTAGCCTGCTGCTGAAGG
  ACATTGTTCAGCACCACGCCCTGCGGCCTTGCTATGATTGGGTGATATACAACACCACCCCTGAGCATGA
  GGCCCTGCAGTGGTCCTATCTGCATCCTAGAGATGGGCTGATGCCAATGTATCTCAACATTCAGTCACAT
  CTTTACCGGGTACTCGAGAAAATTCACCGCGTCTTGAACGATAGAGGACGATGGTCACGAGCCTACAGAG
  CCCGGAAGATAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100466 representing YP_068022
  Red=Cloning sites Green=Tags
  
  METRGRRSGAVLDQPDEPEAHPRKRPARRAPLYRDGDHPDADAATLEGPDPGRAGRPSSGALLPQSPQPA
  KRGGLLDRDAVEHITELWDRLELLQQTLSKMPMADGLKPLKNFASLQELLSLGGERLLAELVRENMHVRQ
  MMNEVAPLLREDGSCVSLNYHLQPVIGVIYGPTGCGKSQLLRNLLSAQLISPAPETVFFIAPQVDMIPPS
  ELKAWEMQICEGNYAPGIEGTFVPQSGTLRPKFIKMAYEDLTQDHNYDVSDPRNVFAQAAAHGPIAIIMD
  ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDLGGNIANLKIQAKMHLISPRMHP
  SQLNRFVNTYTKGLPVAISLLLKDIVQHHALRPCYDWVIYNTTPEHEALQWSYLHPRDGLMPMYLNIQSH
  LYRVLEKIHRVLNDRGRWSRAYRARKIK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068022
• RefSeq ORF: 1344
• Locus ID: 2958471
• MW: 50.5 kDa