E2B (NC_003266) Virus Tagged ORF Clone

CAT#: VC100467

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase [Human adenovirus E], codon optimized for human cell expression, YP_068023

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100467 represents NCBI reference of YP_068023 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCGTGCAGACCCATGGGAGCCGCGGCCTCCACCCCGAGGCTAGTGATCCTGGTCGCCAACCAT
  CCAGGAGAAGATCCCGCCAGAGCTCTCCCGGTGCCGTTCCCGAACCTGCCAGGGCAAGACGGCGACGCGC
  ACCTGCCACAACTGCAAGCGGAAGCAGAGCAGCACCTACAGCTCGCAGAGCTTCTTCACCTCCCCTGCTG
  ACTATGGAGGCTAAACCTTTGCCACCTGCTAAAAAGAAGCGAGGCACCGTTGTTACCCCCCAGGGGCATG
  GCACCCTTCAAGCAATTGACGTGGCTACGAATGGCGCAGTGGAAATTAAATATCACCTTGACCTGCCACG
  AGCCCTGGAGAAGCTGCTGCAGGTCAACAGGGCTCCCCCTCTCCCAACTGATCTCACCCCTCAAAGGCTG
  AGGACACTGGATAGCAGTGGCCTCAGGGCCTTGGTCCTGGCCTTGCGGCCAGTCAGGGCTGAGGTCTGGA
  CCTGCCTGCCAAGGGGCTTGGTAAGCATGACAACAATCGAGGCCGAGGAGGGCCAGGCAGATCACCATGA
  CGTCGTGCAGCATCAAATGCAGGCACCGAGGCTCCATTTCCCTCTTAAGTTCCTCGTGAAGGGTACCCAG
  GTTCAGCTCGTACAACACGTACATCCAGTCCAGAGGTGTGAACACTGCGGGAGGCTGTACAAACACAAGC
  ACGAGTGTAGCGCTCGGAGACGCCACTTCTATTTTCACCACATCAACAGCCATTCCTCAAATTGGTGGCA
  GGAGATCCAATTCTTCCCAATTGGCTCCCACCCAAGAACCGAGCGGCTGTTTCTCACTTACGATGTCGAA
  ACATACACCTGGATGGGAAGCTTCGGCAAGCAGCTTGTGCCGTTCATGCTGGTAATGAAGCTGTCAGGCG
  ATGACCGGCTGGTGGAATTGGCCCTCGACCTCGCCCTGCAGCTGAAGTGGGATAGATGGCATGGCGACCC
  TCGCACTTTCTACTGTGTGACACCTGAAAAGATGGCGGTGGGCCAACAGTTCCGACAGTACAGAGACCGG
  CTCCAGACTGCACTTGCTGTGGACCTGTGGACTAGTTTCTTGCGCGCTAACCCTCACCTGGCTGATTGGG
  CCTTGGAACAGCACGGCCTGTCAGACCCGGATGAGCTTACTTACGAGGAGCTCAAGAAGCTCCCTCATGT
  CAAGGGAAGACCCCGGTTCGTGGAATTGTATATTGTGGGCCACAACATAAACGGCTTCGACGAGATTGTT
  CTCGCCGCCCAGGTGATCAACAATCGCGCCGAAGTACCCCAGCCCTTCCGGATAACGAGGAATTTTATGC
  CAAGAGCAGGCAAAATTCTGTTCAATGACGTGACTTTCGCCCTCCCTAATCCCGCATACAAAAAACGAAC
  AGACTTTCAGCTTTGGGAGCAGGGGGGCTGCGACGACATTGACTTTAAACATCAGTTTTTGAAGGTAATG
  GTCCGGGATACCTTCGCTCTGACTCACACATCTCTGCGGAAGGCAGCACAGGCGTATGCCCTGCCAGTGG
  AGAAAGGATGTTGTGCTTACAAGGCCGTAAATCAATTTTACATGCTGGGCAGTTATAGAGCAGATCAGGA
  TGGATTCCCACTCGAGGAATATTGGAAGGACAGGGAGGAGTTCTTGTTGAATCGCGAACTCTGGAAGCAG
  AAAGGGCAGCTGAAGTACGACATAATACAGGAAACTCTGGATTACTGTGCACTTGATGTGCTGGTGACAG
  CTGAGCTTGTAGCTAAGTTGCAGGATAGCTACGCACATTTTATTCGCGACTCAGTGGGGCTGCCACACGC
  CCATTTTAACATTTTCCAGAGACCGACTATCTCTTCCAATTCCCACGCGATATTTAGACAGATTGTGTAC
  AGGGCCGAAAAGCCATCCAGAGCCAACCTCGGGGCCGGACTTTTGGCACCATCCCATGAACTTTACGACT
  ACGTAAGAGCATCCATTCGAGGCGGGAGGTGTTACCCCACGTACATTGGGATCCTTGATGAACCACTTTA
  CGTTTACGACATCTGCGGTATGTATGCCAGCGCCCTCACTCACCCAATGCCGTGGGGGACGCCACTCTCC
  CCCTACGAACGAGCCCTGGCCGTTAGAGAGTGGCAAGCCTCATTGGATGATCTTGGAACTTGTATTAGTT
  ATTTCGACCCCGATCTGCTGCCGGGTATCTTTACAATCGACGCAGATCCCCCTGACGAATTGATGTTGGA
  CCCGCTTCCACCATTTTGTTCACGGAAAGGCGGGCGGCTCTGCTGGACGAATGAGCCATTGCGGGGCGAG
  GTGGCTACTTCAGTTGACCTTATCACCCTCCATAATCGGGGGTGGCAGGTCCGGATTGTGCCCGATGAGC
  TGACTACAGTTTTCCCTGAATGGAAGTGTGTCGCTAGAGAATACGTTCAGCTGAACATAGCTGCCAAAGA
  ACGCGCAGATAAAGAGAAAAACCAGACTATGCGATCCATTGCAAAGCTGCTCAGTAACGCACTCTACGGA
  AGCTTTGCTACCAAACTGGATAACAAAAAGATCGTATTCTCTGATCAGATGGACGAAGGACTCCTGAAGG
  GTATTTCTGCTGGTACAGTCAATATTAAGAGCAGTTCATTTCTTGAGACCGACAACTTGTCAGCTGAAGT
  TATGCCCGCCTTTGAGAGGGAATACCTGCCACAACAACTGGCCCTGCTGGATTCCGATCCAGAAGACAGC
  GAGGACGAGCAGCGGCCAGCTCCATTTTACACGCCACCTGCCGGGACGCCTGGGCACGTGGCATACACAT
  ATAAACCCATTACATTTCTGGATGTCGATGAGGGTGATATGTGTCTGCATACTCTGGAAAAGGTGGACCC
  ACTTGTTGACAACGACAGGTACCCCTCCCATGTCGCAAGCTTCGTGCTGGCGTGGACGCGCGCCTTTGTC
  TCCGAATGGAGCGGTTTCTTGTATGATGAGGATCGAGGAGTGCCCCTGGAAGATAGGCCCATCAAGTCTG
  TTTATGGCGACACTGACAGCCTGTTCGTTACCCAACGCGGCCACGAGCTTATGGAAACGCGCGGAAAGAA
  GCGGATCAAGAAGAACGGAGGCAAGCTGGTATTCGACCCCAATCAACCGGATCTGACCTGGCTCGTGGAG
  TGCGAAACGGTTTGTGCACACTGTGGGGCCGACGCTTACGCCCCTGAATCTGTGTTCCTCGCCCCAAAGC
  TGTATGCTCTGAAGAGCCTGCTTTGCCCTGCCTGCGGGCAAACCTCAAAAGGAAAGCTCAGAGCTAAGGG
  GCACGCCGCTGAGGCACTGAACTATGAGCTGATGGTTAATTGCTACCTCGCGGACGCGCAGGGTGCGGAC
  AGGGAACGCTTTTCAACGTCTAGGATGTCTTTGAAGCGCACATTGGCCAGTGCCCAGCCCGGCGCACACC
  CCTTTACAGTTACAGAGACTACTCTCACAAGAACCCTGAGACCCTGGAAAGATCGCACCTTGGCTGCCCT
  GGATGCTCACCGCCTCGCTCCATATAGCCGAAGCAGGCCTAACCCGCGGAATGAGGAAGTCTGTTGGATT
  GAAATGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100467 representing YP_068023
  Red=Cloning sites Green=Tags
  
  MALVQTHGSRGLHPEASDPGRQPSRRRSRQSSPGAVPEPARARRRRAPATTASGSRAAPTARRASSPPLL
  TMEAKPLPPAKKKRGTVVTPQGHGTLQAIDVATNGAVEIKYHLDLPRALEKLLQVNRAPPLPTDLTPQRL
  RTLDSSGLRALVLALRPVRAEVWTCLPRGLVSMTTIEAEEGQADHHDVVQHQMQAPRLHFPLKFLVKGTQ
  VQLVQHVHPVQRCEHCGRLYKHKHECSARRRHFYFHHINSHSSNWWQEIQFFPIGSHPRTERLFLTYDVE
  TYTWMGSFGKQLVPFMLVMKLSGDDRLVELALDLALQLKWDRWHGDPRTFYCVTPEKMAVGQQFRQYRDR
  LQTALAVDLWTSFLRANPHLADWALEQHGLSDPDELTYEELKKLPHVKGRPRFVELYIVGHNINGFDEIV
  LAAQVINNRAEVPQPFRITRNFMPRAGKILFNDVTFALPNPAYKKRTDFQLWEQGGCDDIDFKHQFLKVM
  VRDTFALTHTSLRKAAQAYALPVEKGCCAYKAVNQFYMLGSYRADQDGFPLEEYWKDREEFLLNRELWKQ
  KGQLKYDIIQETLDYCALDVLVTAELVAKLQDSYAHFIRDSVGLPHAHFNIFQRPTISSNSHAIFRQIVY
  RAEKPSRANLGAGLLAPSHELYDYVRASIRGGRCYPTYIGILDEPLYVYDICGMYASALTHPMPWGTPLS
  PYERALAVREWQASLDDLGTCISYFDPDLLPGIFTIDADPPDELMLDPLPPFCSRKGGRLCWTNEPLRGE
  VATSVDLITLHNRGWQVRIVPDELTTVFPEWKCVAREYVQLNIAAKERADKEKNQTMRSIAKLLSNALYG
  SFATKLDNKKIVFSDQMDEGLLKGISAGTVNIKSSSFLETDNLSAEVMPAFEREYLPQQLALLDSDPEDS
  EDEQRPAPFYTPPAGTPGHVAYTYKPITFLDVDEGDMCLHTLEKVDPLVDNDRYPSHVASFVLAWTRAFV
  SEWSGFLYDEDRGVPLEDRPIKSVYGDTDSLFVTQRGHELMETRGKKRIKKNGGKLVFDPNQPDLTWLVE
  CETVCAHCGADAYAPESVFLAPKLYALKSLLCPACGQTSKGKLRAKGHAAEALNYELMVNCYLADAQGAD
  RERFSTSRMSLKRTLASAQPGAHPFTVTETTLTRTLRPWKDRTLAALDAHRLAPYSRSRPNPRNEEVCWI
  EMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 3579 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068023
• RefSeq ORF: 3579
• Locus ID: 2958478
• MW: 135.2 kDa