L1 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100469

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 52K [Human adenovirus E], codon optimized for human cell expression, YP_068025

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100469 represents NCBI reference of YP_068025 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCGGTGTTGCGACAAATGAGGCCTCACCCACCACCCCAGCAACAGCCTCCACCCACACAGCAAC
  CAGCCCTGCTTCCCCCGCCTCAGCAGCAGCAATTGCCAGCCACAACCGCAGCAGCCGCCGTGTCCGGAGC
  TGGTCAATCACAATATGACCTGGCTCTGGAGGAGGGGGAGGGACTCGCCAGACTGGGCGCCAGCTCTCCC
  GAGCGCCACCCCCGCGTGCAAATGAAACGGGATGCCCGGGAGGCATATGTGCCTAAACAGAACCTGTTCC
  GAGACCGCTCCGGCGAGGAGCCCGAGGAGATGAGGGCCGCTAGATTTCACGCAGGTCGCGAACTTAGAAG
  GGGCCTTGACCGGAAAAGAGTTCTGAGGGACGAGGACTTTGAGGCGGATGAACTCACCGGAATCAGCCCG
  GCTAGGGCTCACGTGGCAGCCGCCAATCTCGTCACCGCTTATGAGCAGACCGTAAAAGAAGAAAGTAACT
  TTCAGAAAAGCTTTAACAACCACGTTAGGACTCTTATCGCCAGGGAAGAGGTAACGCTGGGACTCATGCA
  TCTGTGGGACTTGCTCGAGGCTATCGTGCAAAACCCCACCTCCAAACCTCTGACTGCTCAGCTTTTCCTG
  GTGGTGCAACACTCCCGGGACAACGAAACTTTTCGGGAGGCTTTGCTGAATATTACAGAGCCCGAAGGGA
  GATGGCTGCTGGATTTGGTCAACATACTGCAGAGCATCGTAGTTCAGGAAAGAGGTCTCCCGCTCTCAGA
  GAAACTCGCCGCTATTAACTTTTCCGTGCTCAGCCTGGGCAAGTACTACGCTAGAAAGATTTACAAAACC
  CCTTATGTGCCGATAGATAAAGAGGTAAAGATTGATGGATTTTACATGCGAATGACTCTTAAAGTCCTTA
  CACTTAGCGACGATCTCGGGGTGTACCGGAATGACCGGATGCGCAGAGCCGTTTCTGCAAGCAGGAGACG
  AGAGCTGAGTGATCAGGAACTTATGCACAGTCTGCAGCGAGCCCTGACTGGGGCCGGAACCGAAGGCGAG
  TCTTATTTCGACATGGGCGCTGATCTGCATTGGCAACCCAGTCGGCGCGTCTTGGAAGCAGCAGCGGTTC
  CTTACGTCGAGGAAGTGGACGACGAGGATGAAGGAGAATACCTGGAGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100469 representing YP_068025
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPHPPPQQQPPPTQQPALLPPPQQQQLPATTAAAAVSGAGQSQYDLALEEGEGLARLGASSP
  ERHPRVQMKRDAREAYVPKQNLFRDRSGEEPEEMRAARFHAGRELRRGLDRKRVLRDEDFEADELTGISP
  ARAHVAAANLVTAYEQTVKEESNFQKSFNNHVRTLIAREEVTLGLMHLWDLLEAIVQNPTSKPLTAQLFL
  VVQHSRDNETFREALLNITEPEGRWLLDLVNILQSIVVQERGLPLSEKLAAINFSVLSLGKYYARKIYKT
  PYVPIDKEVKIDGFYMRMTLKVLTLSDDLGVYRNDRMRRAVSASRRRELSDQELMHSLQRALTGAGTEGE
  SYFDMGADLHWQPSRRVLEAAAVPYVEEVDDEDEGEYLED
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068025
• RefSeq ORF: 1170
• Locus ID: 2958472
• MW: 44.3 kDa