E1B (NC_003266) Virus Tagged ORF Clone

CAT#: VC100464

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 55K [Human adenovirus E], codon optimized for human cell expression, YP_068020

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100464 represents NCBI reference of YP_068020 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCCGCAACCCTTTCCAGCAGGGCTTGCCGGCAGGATTCTTGTCCTCAAGTTTTGTCGAGAACA
  TGGAGGTCCCTGCTCCCGAATGTAACCTGCGCTTGCTCGCTGGCACGGCTGCCAGACACAGCGAGGACCC
  AGAAAGTCCAGCTGCTGGCGGTAGTCGGCGCGAGTCTGAGTCTCGGCCCGGCCCCAGCGGTGGTGGAGTG
  GCAGACCTGTTTCCAGAACTGCATAGAGTGTTGACCCGGAGTTCCTCTGGACGAGAAAGAGGAATAAAAC
  GGGAGAGGCACGATGAGACCAATCACAGAATCGAACTTACAGTCGGTTTGATGTCTCGGAAGCGCCCCGA
  AACTGTGTGGTGGTATGAGGTTCAGTCAACTGGGACAGACGAGGTCTCAGTGATGCACGAGAGATTCTCA
  CTGGAACAGGTGAAAACATGTTGGCTTGAACCTGAGGATGACTGGGAGGTGGCAATTCGCAATTATGCTA
  AGCTCGCCTTGCGCCCTGACCGGAAGTATAAGATCACAAAGCTCATCAATATTCGGAACGCTTGTTACAT
  CTCCGGGAATGGCGCAGAAGTTGAGATCTGCCTCCAGGATCGGGTGGCATTCAGATGTTGCATGATGAAC
  ATGTATCCCGGCGTCGTGGACATGGACGGCGTCACCTTTATGAACATGAGGTTCCGGGGGGATGGGTATA
  ACGGGACCGTGTTTATGGCCAATACCAAGCTTACCGTTCATGGGTGCTCTTTCTTCGGTTTCAATAACAC
  ATGTATCGAGGCCTGGGGTCAGGTCGGCGTGAAAGGATGCTCATTTTCTGCCAACTGGATGGGTGTGGTC
  GGGCGAACAAAGTCAATGCTGAGCGTGAAGAAGTGCCTGTTCGAACGGTGTCATCTGGGCGTGATGAGTG
  AGGGGGAGGCACGCATCCGCCACTGCGCCAGCACCGAGACAGGGTGTTTCGTCCTGTGTAAGGGGAACGC
  AAAGATAAAGCACAACATGATTTGTGGAGCTTCAGACGAGCGGGGCTATCAGATGTTGACCTGTGCTGGG
  GGGAACTCCCACATGCTGGCAGCTGTGCACGTGGCGAGCCATTCAAGGAAGCCCTGGCCAGAGTTCGAAC
  ACAATGTCATGACCAGGTGCAACATGCACCTTGGCGCCAGACGGGGAATGTTCATGCCTTATCAGTGTAA
  CCTGAATTACGTGAAGGTGCTGCTTGAGCCCGACGTGATGTCTCGCGTTAGCCTTACAGGAGTTTTTGAT
  ATGAATGTAGAGGTTTGGAAAATTCTCCGCTACGACGAGTACAAGACGAGGTGTCGCGCTTGTGAGTGTG
  GCGGTAAACATGCGCGCTTCCAGCCGGTTTGCGTAGACGTTACCGAGGATCTCCGACCTGATCATCTCGT
  GCTGAGCTGCACCGGAACAGAGTTTGGTTCTAGCGGCGAGGAGAGCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100464 representing YP_068020
  Red=Cloning sites Green=Tags
  
  MESRNPFQQGLPAGFLSSSFVENMEVPAPECNLRLLAGTAARHSEDPESPAAGGSRRESESRPGPSGGGV
  ADLFPELHRVLTRSSSGRERGIKRERHDETNHRIELTVGLMSRKRPETVWWYEVQSTGTDEVSVMHERFS
  LEQVKTCWLEPEDDWEVAIRNYAKLALRPDRKYKITKLINIRNACYISGNGAEVEICLQDRVAFRCCMMN
  MYPGVVDMDGVTFMNMRFRGDGYNGTVFMANTKLTVHGCSFFGFNNTCIEAWGQVGVKGCSFSANWMGVV
  GRTKSMLSVKKCLFERCHLGVMSEGEARIRHCASTETGCFVLCKGNAKIKHNMICGASDERGYQMLTCAG
  GNSHMLAAVHVASHSRKPWPEFEHNVMTRCNMHLGARRGMFMPYQCNLNYVKVLLEPDVMSRVSLTGVFD
  MNVEVWKILRYDEYKTRCRACECGGKHARFQPVCVDVTEDLRPDHLVLSCTGTEFGSSGEESD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068020
• RefSeq ORF: 1449
• Locus ID: 2958470
• MW: 54.2 kDa