L1 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100470

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pIIIa [Human adenovirus E], codon optimized for human cell expression, YP_068026

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100470 represents NCBI reference of YP_068026 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAGCAACCCCCTCCTGACCCAGCAATGCGGGCCGCCTTGCAGTCCCAGCCCTCAGGGATAAACA
  GTTCTGACGACTGGACTCAGGCTATGCAACGGATCATGGCGCTGACTACCCGCAATCCTGAGGCTTTTCG
  CCAACAACCCCAGGCTAACCGACTGAGCGCTATCCTGGAGGCCGTCGTCCCTTCCAGGTCTAATCCAACA
  CATGAGAAGGTCCTTGCAATCGTGAATGCTCTGGTCGAAAACAAGGCCATACGGGGAGACGAGGCCGGCC
  TTGTTTATAATGCACTGTTGGAGAGAGTGGCTAGGTATAACAGTACAAACGTGCAGACGAATCTCGATCG
  GATGGTCACTGACGTGCGCGAGGCCGTTGCCCAAAGAGAGAGATTTCATAGGGAGTCTAACCTGGGCAGC
  ATGGTGGCACTGAACGCATTCCTTTCAACGCAGCCTGCTAATGTGCCAAGAGGGCAGGAGGACTATACGA
  ACTTTATCTCCGCACTCCGCCTTATGGTCACCGAGGTACCACAGTCCGAAGTGTATCAAAGTGGCCCGGA
  CTATTTCTTTCAGACCAGCCGGCAGGGCCTCCAGACGGTTAACCTCTCCCAAGCCTTTAAGAATTTGCAG
  GGGCTTTGGGGGGTGCAGGCACCCGTGGGCGACAGGGCCACGGTGTCTAGTCTCCTTACTCCGAATAGCC
  GCCTTCTCCTGCTCCTGGTGGCCCCTTTTACTGACAGTGGGAGTATTAACCGCAACAGCTACTTGGGCTA
  TCTCATAAACTTGTACCGGGAGGCGATCGGCCAGGCCCATGTCGATGAGCAGACCTATCAGGAGATCACC
  CATGTCTCCCGCGCTCTGGGCCAGGACGATCCCGGCAACCTCGAGGCAACACTGAACTTCCTTCTGACTA
  ATCGGTCTCAAAAGATCCCCCCTCAGTACGCCTTGAGCGCGGAGGAGGAGCGAATCCTCAGATATGTGCA
  GCAAAGCGTGGGGCTCTTTCTGATGCAGGAGGGGGCCACTCCAAGTGCGGCACTGGATATGACCGCTCGG
  AACATGGAACCGAGTATGTATGCCAGCAATAGACCATTTATCAATAAACTGATGGATTATCTCCACCGAG
  CGGCGGCGATGAATTCCGACTACTTCACGAATGCCATCCTCAACCCTCATTGGCTTCCCCCTCCTGGGTT
  CTACACTGGTGAGTATGACATGCCTGACCCCAACGACGGTTTTCTCTGGGATGATGTGGACAGCTCTGTA
  TTTAGCCCACGGCCAGGCGCCAATGAGCGCCCACTCTGGAAAAAGGAGGGATCAGACAGAAGGCCTAGCA
  GTGCGCTGTCCGGAAGAACCGGTGCCGCCGCAGCCGTGCCAGAGGCAGCCAGTCCTTTTCCCTCACTGTC
  ACTGAACTCAGTCCGATCCAGCGAGCTCGGTCGAATTACGCGGCCCAGACTCCTCGGCGAGGAGGAATAC
  TTGAACAACAGCCTTCTCCGGCCCGAGAGGGAGAAAAATTTCCCCAACAACGGGATCGAAAGCCTTGTGG
  ACAAGATGAGCAGATGGAAGACTTATGCCCACGAGCACCGCGACGACCCAAGAGCTACCCAGGGGGCAAC
  AAGCAGGGGCAGCGCCGCTCGCAAGAGACGATGGCACGACAGACAGCGAGGTCTGATGTGGGACGACGAA
  GACTCTGCCGACGACTCCTCTGTCCTTGACCTGGGGGGTTCTGGAGGCGGAAATCCCTTTGCTCATTTGA
  GGCCCCGGGTCGGTCGGCTGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100470 representing YP_068026
  Red=Cloning sites Green=Tags
  
  MQQQPPPDPAMRAALQSQPSGINSSDDWTQAMQRIMALTTRNPEAFRQQPQANRLSAILEAVVPSRSNPT
  HEKVLAIVNALVENKAIRGDEAGLVYNALLERVARYNSTNVQTNLDRMVTDVREAVAQRERFHRESNLGS
  MVALNAFLSTQPANVPRGQEDYTNFISALRLMVTEVPQSEVYQSGPDYFFQTSRQGLQTVNLSQAFKNLQ
  GLWGVQAPVGDRATVSSLLTPNSRLLLLLVAPFTDSGSINRNSYLGYLINLYREAIGQAHVDEQTYQEIT
  HVSRALGQDDPGNLEATLNFLLTNRSQKIPPQYALSAEEERILRYVQQSVGLFLMQEGATPSAALDMTAR
  NMEPSMYASNRPFINKLMDYLHRAAAMNSDYFTNAILNPHWLPPPGFYTGEYDMPDPNDGFLWDDVDSSV
  FSPRPGANERPLWKKEGSDRRPSSALSGRTGAAAAVPEAASPFPSLSLNSVRSSELGRITRPRLLGEEEY
  LNNSLLRPEREKNFPNNGIESLVDKMSRWKTYAHEHRDDPRATQGATSRGSAARKRRWHDRQRGLMWDDE
  DSADDSSVLDLGGSGGGNPFAHLRPRVGRLM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068026
• RefSeq ORF: 1773
• Locus ID: 2958472
• MW: 65.9 kDa