E4 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100494

Myc-DDK-tagged ORF clone of viral ORF for control protein E4 34K [Human adenovirus E], codon optimized for human cell expression, YP_068050

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100494 represents NCBI reference of YP_068050 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGCAATAGTTCTATTATGACAAGATCAAGGACCCGCCTGGCCCTCAGCAGACATCACCCATACC
  AACCTCCCGCAACTCTGCCAAGATGTGAGGAGACTGAATCACGCGCTAGTCTGGTCGAGGACCATCCTGT
  GTTGCCCGACTGCGACACACTGAGTATGCACAACGTGAGCAGCGTAAGAGGCCTGCCTTGTTCTGCTGGA
  TTCACGGTCCTGCAAGAATTTCCTGTACCCTGGGACATGGTACTGACGCCTGAAGAGCTGAGGGTCCTGA
  AGACCTGTATGTCAGTCTGTCTGTGCTGCGCTAACATTGACCTGTTCTCAAGCCAACTTATCCACGGCCG
  AGAGCGATGGGTGATACATTGTCACTGCCAGAATCCTGGTAGCCTGCGGTGCATGGCCGGCGGCACTGTG
  CTTGCTATCTGGTTTCGCAGGATCATCCAAGGATGTATGTTTAACCAAAGAGTCATGTGGTACCGGGAAG
  TTGTTAACTTGCACATGCCAAAGGAGATTATGTATATGGGATCCGTCTTCTGGAGAGGCCGCCACTTGAT
  ATACATCCGCATCTGGTACGACGGCCATGTGGGTTCCATCGTGCCCCAAATGTCATTTGGGTGGTCTACA
  CTCAACTACGGCCTGCTGAACAACCTTGTGGTCCTGTGCTGTACCTACTGTTCAGACTTGAGCGAGATTC
  GGATTCGCTGCTGCGCCAGACGAACCCGCCGCTTGATGCTTCGGGCCATCGGAATCATGCGCAGGGAAAG
  TCTGGATCCAGACCCCTTGAGTAGCAGCCTGACCGAGAGACGGCGCCAGCGGCTTCTGAGAGGTCTGATG
  AGGCACAATAGGCCAATCCCCTTTGCAGATTACGACTCACACAGAAGCTATTCCCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100494 representing YP_068050
  Red=Cloning sites Green=Tags
  
  MSGNSSIMTRSRTRLALSRHHPYQPPATLPRCEETESRASLVEDHPVLPDCDTLSMHNVSSVRGLPCSAG
  FTVLQEFPVPWDMVLTPEELRVLKTCMSVCLCCANIDLFSSQLIHGRERWVIHCHCQNPGSLRCMAGGTV
  LAIWFRRIIQGCMFNQRVMWYREVVNLHMPKEIMYMGSVFWRGRHLIYIRIWYDGHVGSIVPQMSFGWST
  LNYGLLNNLVVLCCTYCSDLSEIRIRCCARRTRRLMLRAIGIMRRESLDPDPLSSSLTERRRQRLLRGLM
  RHNRPIPFADYDSHRSYSR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068050
• RefSeq ORF: 897
• Locus ID: 2958467
• MW: 34.7 kDa