L2 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100471

Myc-DDK-tagged ORF clone of viral ORF for penton base [Human adenovirus E], codon optimized for human cell expression, YP_068027

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100471 represents NCBI reference of YP_068027 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGGCGGGCATACCCGGAAGGCCCACCTCCCTCTTACGAATCCGTTATGCAGCAGGCTATGGCCG
  CCGCTGCAGCCATGCAGCCCCCCCTGGAAGCCCCTTATGTTCCCCCTCGATACCTTGCCCCTACAGAGGG
  CAGAAACTCTATCAGGTACTCCGAGCTGGCCCCACTCTACGATACAACTAGACTCTATCTTGTGGACAAT
  AAGAGTGCCGATATAGCCAGTCTCAACTATCAGAACGACCATTCAAACTTCCTGACTACAGTGGTGCAGA
  ATAACGACTTTACTCCGACAGAGGCCAGCACCCAAACAATCAATTTCGACGAGAGATCTCGCTGGGGGGG
  GCAGCTGAAAACCATTATGCACACCAATATGCCGAACGTGAATGAATTCATGTACAGTAATAAATTTAAG
  GCGAGAGTGATGGTCAGTCGGAAGACCCCTAACGGCGTGACTGTCGGGGATGACTACGACGGCTCTCAGG
  ACGAGCTGAAGTACGAATGGGTGGAGTTCGAGCTTCCAGAAGGAAACTTCAGCGTTACAATGACCATTGA
  CCTGATGAACAATGCAATTATCGATAATTACCTGGCCGTCGGCAGGCAGAATGGTGTGCTGGAGTCCGAT
  ATTGGCGTGAAGTTCGACACAAGAAATTTCAGACTCGGATGGGACCCCGTGACCGAACTGGTGATGCCGG
  GGGTGTACACCAATGAAGCATTTCACCCCGACATCGTGCTTTTGCCAGGTTGTGGCGTGGATTTTACCGA
  GTCCAGACTCTCCAATATGCTGGGCATACGAAAGCGGCAACCTTTTCAGGAGGGCTTTCAAATCATGTAC
  GAAGATCTCGATGGGGGCAATATTCCGGCTCTGCTTGACGTGGAGGCTTACGAGAAATCTAAGGAAGAAT
  CCGTTGCTGCATCCACTGCGGCTGTCGCTACAGCTAGCACTGAGGTGAGGGGGGATAATTTCGCCTCAGC
  CGCAGCAGTGGCCGCGGCTAAGGCAGACGAAACCGAGTCTAAAATAGTGATCCAGCCGGTAGAGAAAGAT
  TCAAAGGACCGCTCCTACAATGTCCTGAGCGATAAAAAGAATACAGCATATAGGAGCTGGTACCTGGCAT
  ACAATTATGGCGATCACGAAAAAGGTGTGCGGTCATGGACTCTGCTGACGACCTCCGACGTAACCTGCGG
  AGTTGAACAGGTGTATTGGTCACTCCCAGATATGATGCAGGATCCTGTGACTTTCCGCTCCACACGGCAG
  GTTTCCAACTATCCCGTAGTGGGCGCCGAACTGATGCCCGTGTATAGCAAGAGCTTTTTTAATGAACAGG
  CAGTCTACTCCCAACAGCTTAGGGCTTTTACTTCACTTACACACGTATTCAATCGCTTTCCCGAAAACCA
  GATCCTTGTACGACCCCCAGCCCCCACTATCACAACCGTGAGCGAAAATGTACCGGCTCTGACTGACCAC
  GGGACGCTTCCACTTAGGTCATCTATCCGAGGCGTGCAGCGGGTTACCGTGACCGACGCCCGACGGAGGA
  CCTGTCCATATGTGTACAAGGCCCTGGGGATTGTGGCCCCTAGAGTATTGAGTTCAAGGACATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100471 representing YP_068027
  Red=Cloning sites Green=Tags
  
  MMRRAYPEGPPPSYESVMQQAMAAAAAMQPPLEAPYVPPRYLAPTEGRNSIRYSELAPLYDTTRLYLVDN
  KSADIASLNYQNDHSNFLTTVVQNNDFTPTEASTQTINFDERSRWGGQLKTIMHTNMPNVNEFMYSNKFK
  ARVMVSRKTPNGVTVGDDYDGSQDELKYEWVEFELPEGNFSVTMTIDLMNNAIIDNYLAVGRQNGVLESD
  IGVKFDTRNFRLGWDPVTELVMPGVYTNEAFHPDIVLLPGCGVDFTESRLSNMLGIRKRQPFQEGFQIMY
  EDLDGGNIPALLDVEAYEKSKEESVAASTAAVATASTEVRGDNFASAAAVAAAKADETESKIVIQPVEKD
  SKDRSYNVLSDKKNTAYRSWYLAYNYGDHEKGVRSWTLLTTSDVTCGVEQVYWSLPDMMQDPVTFRSTRQ
  VSNYPVVGAELMPVYSKSFFNEQAVYSQQLRAFTSLTHVFNRFPENQILVRPPAPTITTVSENVPALTDH
  GTLPLRSSIRGVQRVTVTDARRRTCPYVYKALGIVAPRVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068027
• RefSeq ORF: 1605
• Locus ID: 2958475
• MW: 60.0 kDa