L2 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100473

Myc-DDK-tagged ORF clone of viral ORF for core protein V [Human adenovirus E], codon optimized for human cell expression, YP_068029

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100473 represents NCBI reference of YP_068029 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAAAAGAAAGTTCAAGGAGGAAATGCTTCAGGTTATCGCACCAGAAATTTACGGGCCAGCCGCGG
  CGGTAAAGGACGAGAGGAATCCCAGAAAGATCAAGCGCGTGAAGAAGGACAAAAAGGAGGAGGATGATGT
  CGATGATATGGTTGAGTTTGTGAGAGAATTCGCACCTCGCAGACGAGTGCAGTGGCGAGGTAGGAAGGTG
  AGGCCCGTGCTTAGACCTGGGACTACAGTCGTGTTTGCCCCAGGTGAACGCTCCGGAACAACCTCTAAGC
  GGAGTTACGACGAGGTGTATGGAGATGAAGATATCCTGGAGCAAGCTGCCGAAAGACTCGGAGAATTTGC
  ATACGGGAAGCGGAACAGACTCGCACCTCTGAAAGAAGAAGTAGTCAGCATTCCCCTCGACCACGGGAAC
  CCTACCCCGAGTCTCAAACCTGTCACACTCCAGCAAGTACTTCCATCCGCTGCACCTAGACGAGGATTGA
  AGCGGGAAGGCGAAGACGTGTATCCGACTATGCAACTGATGGTTCCCAAGAGGCAGAAACTTGAGGACGT
  GTTGGAGACTATGAAGGTGGACCCAGACGTTCAACCGGAGGTGAAAGTGCGGCCCATCAAGCAAGTGGCC
  CCCGGCCTTGGCGTGCAAACCGTTGATATAAAGATACCAACCGAGCCGATGGAAACTCAGACAGAGGTGG
  TCAAGCCCATTACCAGTACAATGGAAGTCCAGACTGACCCCTGGATGCCTGCCGCCCCACGGAAACCCCG
  ACGCAAGTATGGTGCTGCCAGTCTGCTGATGCCGAACTACGCCCTTCACCCTTCAATTATTCCGACACCC
  GGATATAGGGGGACGCGATTCTACCATGGCTATACCGGTTCAAGAAGAAGGAAGACGACTACTAGGAGAA
  GACGACGCCGCACTGCCGCAACACCCGCCGCCGCCTTGGTGCGAAGAGTGTATCGGAGGGGGAGAGCCCC
  TCTTACCCTGCCTAGAGCTCGGTACCATCCCAGCATCACTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100473 representing YP_068029
  Red=Cloning sites Green=Tags
  
  MSKRKFKEEMLQVIAPEIYGPAAAVKDERNPRKIKRVKKDKKEEDDVDDMVEFVREFAPRRRVQWRGRKV
  RPVLRPGTTVVFAPGERSGTTSKRSYDEVYGDEDILEQAAERLGEFAYGKRNRLAPLKEEVVSIPLDHGN
  PTPSLKPVTLQQVLPSAAPRRGLKREGEDVYPTMQLMVPKRQKLEDVLETMKVDPDVQPEVKVRPIKQVA
  PGLGVQTVDIKIPTEPMETQTEVVKPITSTMEVQTDPWMPAAPRKPRRKYGAASLLMPNYALHPSIIPTP
  GYRGTRFYHGYTGSRRRKTTTRRRRRRTAATPAAALVRRVYRRGRAPLTLPRARYHPSITI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 1023 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068029
• RefSeq ORF: 1023
• Locus ID: 2958475
• MW: 39.0 kDa