L4 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100481

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 22K [Human adenovirus E], codon optimized for human cell expression, YP_068037

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100481 represents NCBI reference of YP_068037 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACGCGGTTCTTCCAAGAAACTCAAGGTAGAACTGCCTCCCGTTGAAGACCTCGAGGAAGATTGGG
  AGTCCTCCCAAGCAGAAGAGGAGGAGATGGAGGATTGGGATTCAACCCAGGCCGAAGACTCCCTCCAGGA
  CTCTCTGGAAGATGAGGAAGAGGCCGAAGAGGTGGAGGAGGTGGCCGCCGCCGCCAGACCTTCCAGCTCC
  GCAGAAAAAGCAAGCTCAACTGATACGATTAGCGCTCCCGGTCGGGGTCCAGCTAGGCCTCATAGTCGCT
  GGGACGAGACTGGCCGGTTTCCGAATCCTACAACACAGACAGGAAAGAAGGAACGCCAGGGATACAAAAG
  CTGGCGCGGACATAAAAATGCCATAGTATCTTGCCTGCAGGCCTGCGGCGGCAATATCAGTTTCACTAGG
  AGGTACCTTCTTTTTCATCGGGGTGTTAACTTTCCCCGGAACATCCTGCATTATTACAGACACCTTCACA
  GCCCGTATTACTTCCAGGAGGAAGCTGAAAAAGACAAAACATCCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100481 representing YP_068037
  Red=Cloning sites Green=Tags
  
  MPRGSSKKLKVELPPVEDLEEDWESSQAEEEEMEDWDSTQAEDSLQDSLEDEEEAEEVEEVAAAARPSSS
  AEKASSTDTISAPGRGPARPHSRWDETGRFPNPTTQTGKKERQGYKSWRGHKNAIVSCLQACGGNISFTR
  RYLLFHRGVNFPRNILHYYRHLHSPYYFQEEAEKDKTSS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 537 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068037
• RefSeq ORF: 537
• Locus ID: 2958479
• MW: 20.4 kDa