L2 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100472

Myc-DDK-tagged ORF clone of viral ORF for core protein precursor pVII [Human adenovirus E], codon optimized for human cell expression, YP_068028

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100472 represents NCBI reference of YP_068028 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCATCCTGATATCACCCAGTAACAATACTGGCTGGGGACTGCGCGCCCCCTCTAAGATGTATGGCG
  GCGCGAGACAGAGGTCAACACAGCACCCTGTGAGAGTTAGAGGCCATTTTAGGGCTCCATGGGGCGCTCT
  GAAGGGACGGGTGCGCAGCCGGACCACTGTGGACGATGTGATAGACCAGGTCGTCGCTGACGCTCGGAAC
  TATACGCCGGCAGCAGCGCCCGTGTCTACAGTTGACGCCGTCATCGACTCTGTAGTTAGCGACGCTCGGC
  GCTATGCAAGGGCTAAGAGTAGGAGACGGAGGATCGCCCGGCGGCATAGGTCAACCACTGCCATGCGAGC
  GGCCCGGGCTCTCCTTAGAAGGGCGCGACGAACGGGCAGGAGAGCAATGCTCCGAGCAGCCAGGAGAGCA
  GCAAGTGGGGCTTCTGCTGGTAGAACAAGGCGCCGAGCCGCCACGGCTGCCGCTGCGGCTATCGCCAGTA
  TGTCTCGCCCCCGACGGGGCAATGTGTACTGGGTAAGGGACGCTGCAACAGGCGTTAGAGTACCTGTCCG
  CACAAGGCCGCCACGGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100472 representing YP_068028
  Red=Cloning sites Green=Tags
  
  MSILISPSNNTGWGLRAPSKMYGGARQRSTQHPVRVRGHFRAPWGALKGRVRSRTTVDDVIDQVVADARN
  YTPAAAPVSTVDAVIDSVVSDARRYARAKSRRRRIARRHRSTTAMRAARALLRRARRTGRRAMLRAARRA
  ASGASAGRTRRRAATAAAAAIASMSRPRRGNVYWVRDAATGVRVPVRTRPPRT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 579 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068028
• RefSeq ORF: 579
• Locus ID: 2958475
• MW: 21.3 kDa