E3 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100487

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-delta [Human adenovirus E], codon optimized for human cell expression, YP_068043

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100487 represents NCBI reference of YP_068043 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCACTCTCAACATTGGTCTTCCTGAGCCTCATCGGAATCGTGTTCTCAAACCCCATCCCTCGGG
  TCTCCTTCATCAAACCAGTGAACGTGACCGAAGGGTCCAACGTGACCCTGGTAGGGGTTGAAGGAGCTAA
  AAATACAACCTGGACCAAGTACCATCTGAATGGCTGGAAAGATATCTGCAACTGGTCAGTGACCGTCTAC
  ACATGTGAGGGGGTGAACCTGACTATTGTGAACGCAACATCTGCACAGAACGGCAGGATCCAGGGCCAGA
  GCGTGAGCGATTCTGAGGGGTACTATACACAGCATACCTTCATTTATGATATAAAGGTGATCCCACTCCC
  GACCCCCTCTCCGCCTTCTACAACCCAGACCACCACATCTACTCAGCCCACCGCGACTACCACCGCCGAA
  GCCTTTCTGCTGCTGCCTCCATCTTCAAGTCCAACAGCTTCAACAAATAAACAGACGACCAAATTCCTTA
  GCACGACAGAATCTCACACGACAGCCACTCTCAGGGCCTTTTCATCTACTGCCAACTTGACTTCACTGAG
  TTATACACTGATTAGTAGTGTGACCACATCCACGCCAGCCTCTCTGCCTACTCCACTGAAACAGACTGAA
  GGGGGCCTCCAGTGGCAGATCACCCTCCTTATAGTGATTGGTGTGGTGATCCTGGCCATGCTTCTGTACT
  TCATTTTTTGCCGGCGAATCCCAAATGCAAAACCCGTGTACAAACCGATAGTGATCGGGCAACCAGAACC
  CCTGCAGGTCGAGGGCGGACTGCGCAATTTGCTGTTCTCCTTCACTGTCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100487 representing YP_068043
  Red=Cloning sites Green=Tags
  
  MKALSTLVFLSLIGIVFSNPIPRVSFIKPVNVTEGSNVTLVGVEGAKNTTWTKYHLNGWKDICNWSVTVY
  TCEGVNLTIVNATSAQNGRIQGQSVSDSEGYYTQHTFIYDIKVIPLPTPSPPSTTQTTTSTQPTATTTAE
  AFLLLPPSSSPTASTNKQTTKFLSTTESHTTATLRAFSSTANLTSLSYTLISSVTTSTPASLPTPLKQTE
  GGLQWQITLLIVIGVVILAMLLYFIFCRRIPNAKPVYKPIVIGQPEPLQVEGGLRNLLFSFTVW
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 822 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068043
• RefSeq ORF: 822
• Locus ID: 2958477
• MW: 29.7 kDa