E4 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100493

Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf6/7 [Human adenovirus E], codon optimized for human cell expression, YP_068049

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100493 represents NCBI reference of YP_068049 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGGAATTCCTCTATCATGACACGCAGTAGAACGCGGCTCGCTCTGTCCAGGCATCATCCCTATC
  AGCCCCCAGCCACCCTCCCACGCTGCGAAGAAACTGAGTCACGCGCCAGCCTGGTGGAGGACCACCCTGT
  GTTGCCCGATTGCGATACCCTTTCTATGCACAATATCACTGTCATCCCCATCACAGAAGACTCACCACAG
  TTGCTGAACTGCGAGGTGCAGATGCAGGAGTGTCCCGAGGGCTTCATTTCTCTCACGGACCCGAGACTGA
  GCCGCAGCGAGACTGTATGGAACGTCGAAATTAAGACTATGAGTATTACTAATAGCATCCAGATGTTTAA
  GGCGGTCCGGGGAGAGCGAATCGTATACTCCATGAGGTGGGAGGGCGGAGGCAAAATAACTACCCGGATC
  CTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100493 representing YP_068049
  Red=Cloning sites Green=Tags
  
  MSGNSSIMTRSRTRLALSRHHPYQPPATLPRCEETESRASLVEDHPVLPDCDTLSMHNITVIPITEDSPQ
  LLNCEVQMQECPEGFISLTDPRLSRSETVWNVEIKTMSITNSIQMFKAVRGERIVYSMRWEGGGKITTRI
  L
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 423 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003266.2, YP_068049
• RefSeq ORF: 423
• Locus ID: 2958467
• MW: 16.0 kDa