HE (NC_006577) Virus Tagged ORF Clone

CAT#: VC100570

Myc-DDK-tagged ORF clone of viral ORF for hemagglutinin-esterase glycoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173237

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100570 represents NCBI reference of YP_173237 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGATCATCTTCCTGTTCTTCTACTTCTGCTACGGCTTCAACGAACCCCTGAACGTCGTGTCCCACC
  TGAATCACGACTGGTTTCTGTTCGGCGATAGCCGGAGCGACTGCAACCACATCAACAACCTGAAGATCAA
  GAACTTTGACTACCTGGACATCCACCCCAGCCTCTGCAACAACGGCAAGATCTCCAGCAGCGCCGGAGAC
  AGCATCTTCAAGTCCTTCCACTTCACCCGGTTCTATAACTACACCGGCGAGGGCGACCAGATCATCTTCT
  ACGAGGGCGTGAACTTCAACCCCTACCACCGGTTCAAGTGCTTCCCCAACGGCAGCAACGACGTGTGGCT
  GCTGAACAAGGTGCGGTTCTACCGGGCCCTGTACAGCAACATGGCCTTCTTCCGGTACCTGACCTTCGTG
  GACATCCCATACAACGTGAGCCTGTCCAAGTTCAACAGCTGCAAGAGCGACATTCTCAGCCTCAATAACC
  CCATCTTCATCAACTACAGCAAGGAGGTGTACTTCACACTGCTCGGGTGTAGCCTGTACCTCGTGCCCCT
  GTGCCTGTTCAAGTCCAATTTCAGCCAGTACTACTACAACATCGACACCGGGTCCGTCTACGGCTTTTCC
  AACGTGGTGTACCCAGACCTCGACTGCATCTACATCTCCCTGAAGCCCGGCTCCTACAAGGTGAGCACCA
  CCGCCCCCTTCCTGTCCCTGCCCACCAAGGCCCTGTGCTTCGACAAAAGCAAGCAGTTCGTGCCCGTGCA
  GGTGGTCGACTCCCGGTGGAACAACGAGCGGGCCTCCGACATCAGCCTCAGCGTGGCCTGCCAGCTCCCT
  TACTGCTACTTCCGGAACTCCAGCGCCAACTACGTGGGCAAGTACGACATCAACCACGGCGACAGCGGCT
  TCATCAGCATCCTGAGCGGGCTGCTGTATAACGTCAGCTGCATCAGCTACTACGGCGTGTTCCTGTACGA
  CAACTTCACAAGCATCTGGCCCTATTACTCCTTCGGGCGGTGCCCCACCAGCAGCATCATCAAGCATCCC
  ATCTGCGTGTACGACTTTCTCCCCATTATCCTGCAGGGCATCCTGCTGTGCCTGGCCCTGCTGTTTGTGG
  TCTTCCTGCTGTTCCTGCTCTATAACGACAAATCCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100570 representing YP_173237
  Red=Cloning sites Green=Tags
  
  MLIIFLFFYFCYGFNEPLNVVSHLNHDWFLFGDSRSDCNHINNLKIKNFDYLDIHPSLCNNGKISSSAGD
  SIFKSFHFTRFYNYTGEGDQIIFYEGVNFNPYHRFKCFPNGSNDVWLLNKVRFYRALYSNMAFFRYLTFV
  DIPYNVSLSKFNSCKSDILSLNNPIFINYSKEVYFTLLGCSLYLVPLCLFKSNFSQYYYNIDTGSVYGFS
  NVVYPDLDCIYISLKPGSYKVSTTAPFLSLPTKALCFDKSKQFVPVQVVDSRWNNERASDISLSVACQLP
  YCYFRNSSANYVGKYDINHGDSGFISILSGLLYNVSCISYYGVFLYDNFTSIWPYYSFGRCPTSSIIKHP
  ICVYDFLPIILQGILLCLALLFVVFLLFLLYNDKSH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 1158 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_173237
• RefSeq ORF: 1158
• Locus ID: 3200425
• MW: 44.7 kDa