nsp5 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100580

Myc-DDK-tagged ORF clone of viral ORF for nsp5 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459936

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100580 represents NCBI reference of YP_459936 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGAATAGTCAAGATGGTAAGCCCTACTTCAAAAATCGAACCGTGTATCGTTAGCGTGACTTATG
  GAAGCATGACACTGAATGGCCTGTGGCTGGATGATAAGGTTTATTGCCCCAGGCATGTAATCTGCTCTAG
  CTCAAACATGAATGAGCCTGACTACTCCGCGCTGCTTTGCCGCGTGACCCTGGGGGACTTCACGATTATG
  TCTGGAAGAATGAGCCTTACTGTGGTGAGCTATCAGATGCAGGGCTGTCAACTCGTGCTGACTGTGAGTT
  TGCAGAACCCGTACACTCCGAAGTATACATTCGGCAACGTAAAACCCGGGGAAACATTTACCGTTCTGGC
  GGCATATAATGGTAGACCGCAAGGCGCGTTTCATGTGACCATGCGCAGTAGCTATACTATCAAGGGTTCT
  TTCCTGTGCGGTTCCTGCGGATCTGTTGGCTACGTGCTGACTGGCGATAGCGTTAAGTTCGTGTACATGC
  ACCAACTCGAACTCTCTACAGGATGCCACACCGGAACAGATTTCACCGGTAACTTCTATGGGCCGTACCG
  GGATGCTCAGGTGGTGCAGCTGCCCGTTAAAGATTATGTGCAGACTGTGAATGTAATAGCTTGGCTGTAT
  GCGGCAATTTTGAATAATTGCGCCTGGTTCGTTCAGAATGATGTGTGCAGCACCGAGGACTTTAACGTCT
  GGGCAATGGCAAATGGCTTTTCACAGGTGAAAGCCGATCTTGTGCTGGACGCCCTGGCTTCTATGACGGG
  CGTCAGTATCGAAACCCTCCTCGCCGCCATTAAGCGGCTGTACATGGGGTTTCAAGGACGGCAGATCCTG
  GGCAGCTGCACCTTTGAGGATGAACTGGCCCCCAGTGATGTCTACCAACAGCTCGCTGGGGTCAAGCTGC
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100580 representing YP_459936
  Red=Cloning sites Green=Tags
  
  MSGIVKMVSPTSKIEPCIVSVTYGSMTLNGLWLDDKVYCPRHVICSSSNMNEPDYSALLCRVTLGDFTIM
  SGRMSLTVVSYQMQGCQLVLTVSLQNPYTPKYTFGNVKPGETFTVLAAYNGRPQGAFHVTMRSSYTIKGS
  FLCGSCGSVGYVLTGDSVKFVYMHQLELSTGCHTGTDFTGNFYGPYRDAQVVQLPVKDYVQTVNVIAWLY
  AAILNNCAWFVQNDVCSTEDFNVWAMANGFSQVKADLVLDALASMTGVSIETLLAAIKRLYMGFQGRQIL
  GSCTFEDELAPSDVYQQLAGVKLQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_459936
• RefSeq ORF: 912
• MW: 33.3 kDa