E (NC_006577) Virus Tagged ORF Clone

CAT#: VC100573

Myc-DDK-tagged ORF clone of viral ORF for small membrane protein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173240

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100573 represents NCBI reference of YP_173240 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGACCTGTTCTTCAATGACACAGCCTGGTACATCGGACAAATCCTCGTCCTCGTACTTTTCTGCC
  TTATTAGCTTGATTTTCGTGGTGGCATTCCTTGCCACTATTAAGCTCTGTATGCAGCTGTGTGGTTTCTG
  CAACTTTTTCATTATCTCACCTAGCGCCTATGTTTATAAAAGGGGAATGCAGTTGTACAAGAGCTACAGT
  GAGCAGGTAATCCCTCCTACCAGTGATTACCTCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100573 representing YP_173240
  Red=Cloning sites Green=Tags
  
  MVDLFFNDTAWYIGQILVLVLFCLISLIFVVAFLATIKLCMQLCGFCNFFIISPSAYVYKRGMQLYKSYS
  EQVIPPTSDYLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 246 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_173240
• RefSeq ORF: 246
• Locus ID: 3200430
• MW: 9.5 kDa