N2 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100576

Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid phosphoprotein 2 [Human coronavirus HKU1], codon optimized for human cell expression, YP_173243

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: N2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100576 represents NCBI reference of YP_173243 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAGGTGGAGGCCCCTCTGGAAATCGTCCAAGAGAGCTCCCGGAAGCTGCTGGGACTCACCAACC
  TCAGCGAGATCACAAAGCCCCTGATTGAAGCCGAGAAGCCCAACCTGAACTCCCTGTGCCTGCTCAATCA
  CAAGGAGATCCTGAGCCACATCATCCCAGGCAGCCCCGGCTCCCTGAACTTCAAGAAGGTGGAGACCCTG
  AACTTCCAGATGGTCAAGGAGTTCCCCCTGCTGAGCGAGTACCCCCTCCTCAAGCAGAAAGACATCGGCA
  TCGACACCGCCGGCGTGCTCCTGAAGCAGCTCATGGTGAACAAGAGCTCCTGCTACCGGGATGGCATCAG
  CACTATCTCCGTGCCCGCCCACATGCCTATGCACCCCATGGTGAACCCCAGCAAGGGCAGCAGCGGACTC
  CTGATTACCAAGCTGACACTCCTGCTGCCCCCCATGTTCCGGCAGGGCATCCTGCTGCTCAAAAAGCTCA
  GCCTGCTCGGCTTTCGGCTGGTGCGGTTCTGCCTGAAGGCCATCATGCTGAAAGCCCAGGAGGGCCTCCT
  CCTCATCGTCGACCAGGTGCACGTGCTGAATCATGTCGATCCAATCATTGTGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100576 representing YP_173243
  Red=Cloning sites Green=Tags
  
  MLEVEAPLEIVQESSRKLLGLTNLSEITKPLIEAEKPNLNSLCLLNHKEILSHIIPGSPGSLNFKKVETL
  NFQMVKEFPLLSEYPLLKQKDIGIDTAGVLLKQLMVNKSSCYRDGISTISVPAHMPMHPMVNPSKGSSGL
  LITKLTLLLPPMFRQGILLLKKLSLLGFRLVRFCLKAIMLKAQEGLLLIVDQVHVLNHVDPIIVH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 615 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_173243
• RefSeq ORF: 615
• Locus ID: 3200424
• MW: 22.8 kDa