nsp9 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100583

Myc-DDK-tagged ORF clone of viral ORF for nsp9 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459943

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100583 represents NCBI reference of YP_459943 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAACGAACTGATGCCACACAAGCTTAAGATACAAGTTGTTAATTCCGGCTCAGATATGAATTGTA
  ACATCCCCACCCAGTGTTATTATAACAACGGCTCTAGTGGGCGCATCGTGTACGCCGTGCTCTCCGACGT
  GGATGGCTTGAAATACACAAAAATAATGAAGGACGATGGGAACTGTGTGGTGCTCGAGCTCGATCCACCC
  TGTAAGTTTTCCATCCAGGATGTTAAGGGCTTGAAGATTAAGTACCTCTATTTCATTAAGGGCTGCAACA
  CTCTTGCCAGAGGATGGGTAGTCGGCACACTTTCATCTACAATCCGACTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100583 representing YP_459943
  Red=Cloning sites Green=Tags
  
  MNNELMPHKLKIQVVNSGSDMNCNIPTQCYYNNGSSGRIVYAVLSDVDGLKYTKIMKDDGNCVVLELDPP
  CKFSIQDVKGLKIKYLYFIKGCNTLARGWVVGTLSSTIRLQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 333 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_459943
• RefSeq ORF: 333
• MW: 12.4 kDa