nsp13 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100585

Myc-DDK-tagged ORF clone of viral ORF for nsp13 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459942

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100585 represents NCBI reference of YP_459942 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGTGGGCGCTTGCGTCGTCTGCTCTTCTCAGACTAGTCTGCGCTGCGGTAGTTGCATCAGAAAAC
  CACTTCTTTGTTGCAAGTGCTGTTATGACCATGTGATGGCCACTAACCACAAATATGTGCTCTCAGTAAG
  TCCCTACGTGTGTAACGCACCTAACTGTGACGTTTCCGATGTGACCAAGCTCTACCTCGGAGGCATGAGT
  TATTATTGCGAGAACCATAAACCCCACTACTCCTTCAAGCTGGTGATGAATGGCATGGTGTTTGGACTTT
  ATAAGCAGAGCTGCACCGGCTCACCATACATCGACGATTTCAATAAGATTGCCTCCTGTAAGTGGACCGA
  AGTGGACGATTATGTACTGGCCAACGAATGTATCGAACGATTGAAACTTTTTGCCGCGGAGACACAGAAG
  GCCACCGAAGAAGCCTTCAAACAATCCTACGCTAGTGCTACAATCCAGGAGATTGTGTCTGACCGAGAGG
  TCATCCTCTGTTGGGAAACTGGAAAGGTGAAACCCCCCTTGAACAAGAATTACGTCTTCACAGGTTATCA
  CTTTACATCCACCGGCAAAACCGTCCTCGGGGAGTACGTATTCGACAAGAGCGAGCTGACTAACGGCGTT
  TACTATCGGGCCACAACTACATACAAGCTGTCCATAGGCGACGTGTTCGTACTGACGTCTCACTCAGTGG
  CGTCCCTTTCCGCCCCCACTTTGGTACCTCAGGAAAACTACGCATCAATTAGGTTCTCCTCTGTGTACTC
  CGTGCCATTGGTGTTCCAGAACAACGTGGCTAACTACCAGCACATTGGCATGAAGCGGTACTGCACCGTG
  CAGGGACCTCCCGGGACTGGAAAGAGCCACCTGGCTATCGGGCTGGCTGTCTATTACTACACTGCGAGGG
  TGGTGTATACCGCCGCCAGCCATGCCGCAGTGGATGCCCTGTGCGAAAAGGCGTACAAGTTCCTCAACAT
  AAATGACTGCACAAGAATTATTCCGGCAAAGGTGCGAGTCGACTGTTATGACAAATTTAAAATAAATGAC
  ACAACTTGTAAGTACGTGTTCACAACAATTAATGCGCTCCCCGAGCTCGTGACCGATATAGTGGTAGTAG
  ACGAGGTGAGCATGCTTACTAATTACGAACTCTCAGTTATAAATGCAAGGATCAAGGCTAAACACTACGT
  GTACATTGGAGATCCCGCTCAGCTTCCTGCGCCACGGGTTCTGCTGAGCAAGGGGTCCCTGGAGCCCCGG
  CATTTCAACTCAATTACAAAAATAATGTGCTGTCTTGGCCCTGACATTTTCCTGGGGAACTGTTACCGCT
  GTCCCAAGGAAATCGTGGAGACCGTATCTGCTCTGGTTTATGATAACAAGCTGAAGGCCAAAAATGACAA
  CTCTTCATTGTGCTTTAAGGTGTATTTTAAGGGCCAGACTACGCATGAATCTAGTTCTGCCGTAAATATT
  CAACAGATTTATCTTATTAGCAAATTTCTGAAGGCTAACCCTGTATGGAATAGTGCTGTTTTCATCAGCC
  CTTACAACAGTCAGAACTACGTGGCCAAGAGGGTTCTCGGGGTGCAAACGCAGACTGTCGACAGTGCCCA
  GGGCTCTGAGTACGACTACGTCATCTATTCTCAAACTGCTGAGACTGCCCACTCAGTGAATGTTAACAGA
  TTCAATGTCGCCATCACAAGGGCTAAAAAAGGTATATTTTGCGTGATGTCAAACATGCAGCTTTTCGAGA
  GTTTGAATTTCATTACCCTGCCTCTGGACAAAATCCAGAACCAAACGTTGCCCCGGCTGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100585 representing YP_459942
  Red=Cloning sites Green=Tags
  
  MSVGACVVCSSQTSLRCGSCIRKPLLCCKCCYDHVMATNHKYVLSVSPYVCNAPNCDVSDVTKLYLGGMS
  YYCENHKPHYSFKLVMNGMVFGLYKQSCTGSPYIDDFNKIASCKWTEVDDYVLANECIERLKLFAAETQK
  ATEEAFKQSYASATIQEIVSDREVILCWETGKVKPPLNKNYVFTGYHFTSTGKTVLGEYVFDKSELTNGV
  YYRATTTYKLSIGDVFVLTSHSVASLSAPTLVPQENYASIRFSSVYSVPLVFQNNVANYQHIGMKRYCTV
  QGPPGTGKSHLAIGLAVYYYTARVVYTAASHAAVDALCEKAYKFLNINDCTRIIPAKVRVDCYDKFKIND
  TTCKYVFTTINALPELVTDIVVVDEVSMLTNYELSVINARIKAKHYVYIGDPAQLPAPRVLLSKGSLEPR
  HFNSITKIMCCLGPDIFLGNCYRCPKEIVETVSALVYDNKLKAKNDNSSLCFKVYFKGQTTHESSSAVNI
  QQIYLISKFLKANPVWNSAVFISPYNSQNYVAKRVLGVQTQTVDSAQGSEYDYVIYSQTAETAHSVNVNR
  FNVAITRAKKGIFCVMSNMQLFESLNFITLPLDKIQNQTLPRLH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_459942
• RefSeq ORF: 1812
• MW: 67.6 kDa