nsp1 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100577

Myc-DDK-tagged ORF clone of viral ORF for nsp1 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460018

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100577 represents NCBI reference of YP_460018 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATCAAAACTAGCAAATATGGACTGGGATTCAAATGGGCTCCAGAATTTCGGTGGTTGCTCCCCG
  ACGCCGCTGAGGAACTTGCTTCCCCTATGAAGAGTGACGAGGGAGGACTTTGTCCCTCAACCGGCCAAGC
  CATGGAGAGTGTCGGATTCGTGTACGATAATCATGTGAAGATTGATTGTCGATGCATCCTGGGACAGGAA
  TGGCACGTCCAGAGTAACCTGATCAGGGACATCTTTGTTCACGAGGACCTTCATGTGGTGGAAGTGCTCA
  CAAAAACTGCCGTTAAGAGTGGGACTGCAATCCTGATTAAAAGTCCCCTCCACTCTCTGGGAGGGTTCCC
  GAAAGGCTATGTGATGGGGCTGTTTAGAAGCTACAAGACTAAGCGGTACGTGGTGCATCATCTGAGTATG
  ACCACAAGTACCACAAATTTCGGGGAGGACTTTCTGGGCTGGATAGTACCATTCGGATTTATGCCAAGCT
  ATGTGCACAAATGGTTTCAGTTCTGCCGCCTTTATATCGAGGAAAGCGACCTTATTATATCAAATTTCAA
  ATTTGACGACTACGATTTTTCCGTGGAAGATGCTTACGCAGAGGTACACGCCGAGCCAAAAGGTAAGTAC
  TCCCAGAAGGCATATGCACTGCTTCGGCAGTACAGGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100577 representing YP_460018
  Red=Cloning sites Green=Tags
  
  MMIKTSKYGLGFKWAPEFRWLLPDAAEELASPMKSDEGGLCPSTGQAMESVGFVYDNHVKIDCRCILGQE
  WHVQSNLIRDIFVHEDLHVVEVLTKTAVKSGTAILIKSPLHSLGGFPKGYVMGLFRSYKTKRYVVHHLSM
  TTSTTNFGEDFLGWIVPFGFMPSYVHKWFQFCRLYIEESDLIISNFKFDDYDFSVEDAYAEVHAEPKGKY
  SQKAYALLRQYRG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 669 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_460018
• RefSeq ORF: 669
• MW: 25.6 kDa