nsp8 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100582

Myc-DDK-tagged ORF clone of viral ORF for nsp8 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460020

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100582 represents NCBI reference of YP_460020 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGTTTGTGAATATGGCGTCTTTCGTCGAGTACGAGGTCGCCAAGAAAAACTTGGCCGATGCTA
  AGAATTCCGGTTCTGTGAACCAGCAGCAGATTAAGCAATTGGAGAAGGCTTGCAACATTGCTAAGAGCGT
  TTACGAAAGAGATAAAGCGGTTGCTCGCAAACTGGAGAGAATGGCCGATCTGGCGCTGACAAACATGTAC
  AAGGAGGCTAGGATTAATGATAAGAAATCTAAGGTTGTGAGCGCCCTGCAGACAATGCTCTTTTCAATGG
  TGCGGAAGCTTGACAACCAGGCACTTAACTCCATTCTGGATAACGCAGTGAAGGGTTGCGTGCCTCTCTC
  TGCTATCCCGGCCCTGGCTGCCAACACCTTGACTATCGTGATCCCCGACAAACAAGTGTTCGACAAGGTT
  GTAGATAACGTGTACGTGACTTATGCCGGTTCCGTGTGGCATATTCAAACTGTGCAAGACGCCGACGGAA
  TCAATAAGCAGCTTACAGACATTTCTGTGGATTCTAATTGGCCACTGGTGATCATCGCCAATAGGTATAA
  CGAAGTGGCCAATGCTGTGATGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100582 representing YP_460020
  Red=Cloning sites Green=Tags
  
  MSEFVNMASFVEYEVAKKNLADAKNSGSVNQQQIKQLEKACNIAKSVYERDKAVARKLERMADLALTNMY
  KEARINDKKSKVVSALQTMLFSMVRKLDNQALNSILDNAVKGCVPLSAIPALAANTLTIVIPDKQVFDKV
  VDNVYVTYAGSVWHIQTVQDADGINKQLTDISVDSNWPLVIIANRYNEVANAVMQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 585 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_460020
• RefSeq ORF: 585
• MW: 21.6 kDa