nsp2 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100578

Myc-DDK-tagged ORF clone of viral ORF for nsp2 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459934

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100578 represents NCBI reference of YP_459934 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTAAACCCGTCCTGTTTGTGGATCAGTATGGGTGCGATTATAGCGGTAAGCTCGCCGATTGTCTTC
  AAGCCTACGGACACTATAGCTTGCAGGACATGCGCCAGAAGCAGTCAGTGTGGCTGGCAAACTGTGATTT
  CGATATTGTGGTGGCATGGCACGTGGTGAGGGATTCTCGCTTCGTTATGAGGCTTCAGACAATTGCCACC
  ATCTGCGGTATAAAATACGTGGCCCAGCCCACCGAAGATGTAGTCGATGGCGATGTGGTGATCCGCGAGC
  CAGTCCATCTCCTCTCCGCCGATGCTATAGTCCTGAAGCTGCCCAGCCTTATGAAGGTCATGACCCACAT
  GGATGACTTCTCAATTAAATCCATCTACAACGTAGACCTCTGCGACTGCGGCTTCGTGATGCAGTATGGT
  TACGTGGACTGCTTTAATGACAACTGCGATTTTTACGGTTGGGTATCCGGCAACATGATGGACGGATTCA
  GCTGTCCCCTCTGTTGTACTGTTTACGATTCATCAGAGGTGAAGGCCCAGTCATCTGGCGTCATCCCTGA
  AAACCCTGTACTTTTTACCAACAGTACAGACACCGTGAATCATGACTCTTTCAACCTGTACGGCTACAGC
  GTCACACCATTTGGTTCATGCATTTACTGGAGCCCAAGACCCGGACTTTGGATCCCCATCATCAAATCCT
  CCGTTAAATCCTACGACGACCTGGTTTATTCCGGCGTGGTCGGCTGTAAGTCTATCGTGAAGGAAACCGC
  CCTGATAACACACGCACTGTATCTCGATTATGTACAGTGCAAGTGTGGCAACCTGGAACAAAACCACATC
  CTGGGCGTTAACAATAGCTGGTGTCGCCAGCTGCTTCTGAACCGCGGAGATTATAACATGCTGTTGAAGA
  ATATAGATCTCTTCGTGAAGAGACGGGCCGACTTCGCCTGTAAGTTCGCTGTTTGTGGAGATGGGTTCGT
  TCCCTTCCTGCTCGATGGTTTGATACCCCGATCTTACTACCTGATACAAAGTGGAATCTTCTTCACTTCT
  CTGATGAGTCAGTTCAGCCAGGAAGTGTCCGACATGTGCCTCAAGATGTGTATTCTCTTCATGGATAGAG
  TGTCCGTTGCCACGTTCTACATCGAACATTATGTGAACCGACTCGTGACCCAATTCAAGCTGCTGGGCAC
  CACACTGGTGAATAAGATGGTGAACTGGTTCAACACGATGCTGGACGCGAGCGCTCCTGCTACTGGCTGG
  CTCCTTTACCAACTGCTTAATGGACTGTTTGTGGTGAGCCAGGCTAACTTCAATTTTGTGGCCCTTATCC
  CCGACTACGCAAAGATTCTGGTTAACAAGTTCTACACATTCTTCAAACTCCTCCTGGAGTGCGTGACTGT
  AGACGTGTTGAAGGACATGCCAGTCCTGAAAACAATCAATGGCCTGGTGTGTATCGTGGGTAACAAATTC
  TACAACGTGTCTACCGGACTCATCCCCGGCTTCGTGTTGCCCTGTAACGCTCAGGAGCAGCAGATCTATT
  TTTTCGAGGGGGTGGCAGAAAGTGTGATCGTGGAGGATGACGTGATAGAAAATGTTAAAAGCTCACTGTC
  TTCTTACGAATACTGCCAGCCACCAAAGTCCGTCGAAAAAATTTGTATCATCGACAATATGTATATGGGA
  AAGTGCGGCGATAAATTCTTTCCGATCGTCATGAACGATAAGAACATCTGCTTGCTGGATCAGGCCTGGC
  GGTTCCCATGCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100578 representing YP_459934
  Red=Cloning sites Green=Tags
  
  MIKPVLFVDQYGCDYSGKLADCLQAYGHYSLQDMRQKQSVWLANCDFDIVVAWHVVRDSRFVMRLQTIAT
  ICGIKYVAQPTEDVVDGDVVIREPVHLLSADAIVLKLPSLMKVMTHMDDFSIKSIYNVDLCDCGFVMQYG
  YVDCFNDNCDFYGWVSGNMMDGFSCPLCCTVYDSSEVKAQSSGVIPENPVLFTNSTDTVNHDSFNLYGYS
  VTPFGSCIYWSPRPGLWIPIIKSSVKSYDDLVYSGVVGCKSIVKETALITHALYLDYVQCKCGNLEQNHI
  LGVNNSWCRQLLLNRGDYNMLLKNIDLFVKRRADFACKFAVCGDGFVPFLLDGLIPRSYYLIQSGIFFTS
  LMSQFSQEVSDMCLKMCILFMDRVSVATFYIEHYVNRLVTQFKLLGTTLVNKMVNWFNTMLDASAPATGW
  LLYQLLNGLFVVSQANFNFVALIPDYAKILVNKFYTFFKLLLECVTVDVLKDMPVLKTINGLVCIVGNKF
  YNVSTGLIPGFVLPCNAQEQQIYFFEGVAESVIVEDDVIENVKSSLSSYEYCQPPKSVEKICIIDNMYMG
  KCGDKFFPIVMNDKNICLLDQAWRFPCA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_459934
• RefSeq ORF: 1764
• MW: 66.5 kDa