nsp16 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100588

Myc-DDK-tagged ORF clone of viral ORF for nsp16 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460023

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100588 represents NCBI reference of YP_460023 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACAAACGACTGGAAGCCTGGCTATTCCATGCCTGTCCTTTACAAATATTTGAACGTTCCCCTGG
  AAAGGGTGTCTCTGTGGAACTATGGAAAGCCCATCAATCTTCCCACCGGCTGCATGATGAATGTCGCCAA
  GTACACGCAGCTTTGCCAGTACCTGAACACTACCACACTGGCAGTTCCGGTTAACATGAGAGTGCTTCAC
  CTTGGCGCCGGGTCAGACAAGGAAGTGGCTCCCGGTAGCGCCGTGTTGCGGCAGTGGCTCCCGAGCGGCT
  CAATTCTCGTGGACAACGATCTGAATCCTTTCGTGTCAGATAGTCTGGTCACATATTTCGGCGACTGTAT
  GACTTTGCCATTCGATTGTCATTGGGACCTGATAATCTCAGACATGTACGACCCTCTCACAAAGAATATT
  GGAGACTATAATGTGAGCAAAGATGGATTTTTCACTTATATTTGTCACCTGATCCGAGATAAGCTGTCAC
  TCGGTGGGTCCGTGGCCATCAAGATCACTGAATTTTCCTGGAATGCCGACCTCTATAAACTCATGTCATG
  CTTTGCGTTTTGGACCGTCTTTTGTACTAACGTGAACGCAAGTTCAAGTGAGGGCTTTTTGATCGGCATC
  AACTATCTGGGCAAAAGTAGCTTCGAGATCGATGGGAATGTTATGCATGCAAATTACCTGTTTTGGAGGA
  ACAGCACAACTTGGAACGGAGGTGCCTACTCACTGTTCGACATGACTAAATTCTCCCTTAAGCTGGCGGG
  CACAGCCGTCGTGAACCTCAGGCCTGATCAACTGAACGATCTGGTGTACTCTCTGATCGAGAGAGGGAAG
  TTGTTGGTGAGAGATACGCGCAAAGAGATTTTCGTGGGCGATTCTCTCGTGAATACCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100588 representing YP_460023
  Red=Cloning sites Green=Tags
  
  MATNDWKPGYSMPVLYKYLNVPLERVSLWNYGKPINLPTGCMMNVAKYTQLCQYLNTTTLAVPVNMRVLH
  LGAGSDKEVAPGSAVLRQWLPSGSILVDNDLNPFVSDSLVTYFGDCMTLPFDCHWDLIISDMYDPLTKNI
  GDYNVSKDGFFTYICHLIRDKLSLGGSVAIKITEFSWNADLYKLMSCFAFWTVFCTNVNASSSEGFLIGI
  NYLGKSSFEIDGNVMHANYLFWRNSTTWNGGAYSLFDMTKFSLKLAGTAVVNLRPDQLNDLVYSLIERGK
  LLVRDTRKEIFVGDSLVNTC
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 900 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_460023
• RefSeq ORF: 900
• MW: 33.7 kDa