nsp4 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100591

Myc-DDK-tagged ORF clone of viral ORF for nsp4 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459935

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100591 represents NCBI reference of YP_459935 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGTGCTTTCAAATCTGCTCTACATATTGTTCTTCGTCTCCCTTATATGCTTCATCCTGTTGTGGG
  CGCTGCTGCCTACTTATAGTGTCTATAAATCAGACATTCATTTGCCCGCCTATGCCTCATTTAAGGTCAT
  CGATAATGGAGTCGTCCGGGACATTTCTGTGAACGACTTGTGCTTTGCGAATAAGTTTTTCCAATTTGAC
  CAGTGGTACGAGTCAACCTTCGGGTCCGTTTATTACCACAATAGTATGGACTGCCCAATTGTGGTTGCAG
  TCATGGATGAAGATATCGGCAGCACCATGTTTAATGTGCCAACAAAAGTGCTGAGACACGGATTCCACGT
  GTTGCACTTCCTGACATATGCATTCGCCAGCGATAGTGTGCAATGCTATACCCCCCATATCCAAATTAGC
  TATAACGACTTCTACGCGAGTGGGTGTGTTCTGTCCAGCCTGTGCACAATGTTTAAGCGGGGGGATGGGA
  CGCCTCATCCATATTGCTACTCTGATGGAGTTATGAAGAATGCAAGTCTCTACACCAGCCTGGTTCCTCA
  CACAAGATATTCATTGGCAAATAGTAACGGATTTATACGATTTCCCGACGTGATCAGTGAGGGAATCGTT
  AGGATTGTGCGAACTCGAAGCATGACGTATTGCAGAGTGGGGGCCTGTGAGTACGCGGAAGAAGGTATCT
  GTTTCAACTTCAACTCCAGCTGGGTTCTTAACAACGACTACTATAGGAGCATGCCCGGCACCTTCTGTGG
  TCGGGATCTCTTCGACCTGTTTTACCAATTTTTTAGCTCTCTCATCAGGCCTATTGATTTCTTTAGCCTG
  ACAGCTAGCAGCATATTTGGCGCCATCTTGGCTATCGTTGTCGTGCTTGTTTTCTACTACTTGATTAAAC
  TCAAGAGAGCCTTTGGAGACTACACCTCCGTAGTCGTTATCAACGTGGTGGTGTGGTGTATTAATTTTCT
  CATGCTCTTTGTCTTCCAGGTCTATCCAATTTGCGCTTGTGTCTATGCCTGTTTCTACTTTTACGTGACG
  CTGTATTTCCCCTCCGAGATTAGCGTAATAATGCATTTGCAGTGGATTGTGATGTATGGGGCTATCATGC
  CTTTTTGGTTTTGTGTCACCTATGTGGCAATGGTCATCGCCAATCATGTTTTGTGGCTTTTCTCATATTG
  CAGAAAGATCGGTGTGAATGTGTGTAGTGATTCCACTTTTGAAGAAACCTCCCTGACCACCTTCATGATT
  ACCAAAGATTCATATTGCAGACTCAAGAATAGCGTGTCTGATGTGGCCTATAATCGGTATCTCAGCCTGT
  ATAACAAATATCGATACTATTCCGGCAAGATGGACACCGCTGCTTACCGCGAGGCCGCTTGCAGCCAATT
  GGCTAAGGCCATGGAAACCTTCAACCACAATAACGGGAACGATGTCCTCTATCAACCCCCCACCGCATCC
  GTGAGCACAAGCTTCTTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100591 representing YP_459935
  Red=Cloning sites Green=Tags
  
  MVVLSNLLYILFFVSLICFILLWALLPTYSVYKSDIHLPAYASFKVIDNGVVRDISVNDLCFANKFFQFD
  QWYESTFGSVYYHNSMDCPIVVAVMDEDIGSTMFNVPTKVLRHGFHVLHFLTYAFASDSVQCYTPHIQIS
  YNDFYASGCVLSSLCTMFKRGDGTPHPYCYSDGVMKNASLYTSLVPHTRYSLANSNGFIRFPDVISEGIV
  RIVRTRSMTYCRVGACEYAEEGICFNFNSSWVLNNDYYRSMPGTFCGRDLFDLFYQFFSSLIRPIDFFSL
  TASSIFGAILAIVVVLVFYYLIKLKRAFGDYTSVVVINVVVWCINFLMLFVFQVYPICACVYACFYFYVT
  LYFPSEISVIMHLQWIVMYGAIMPFWFCVTYVAMVIANHVLWLFSYCRKIGVNVCSDSTFEETSLTTFMI
  TKDSYCRLKNSVSDVAYNRYLSLYNKYRYYSGKMDTAAYREAACSQLAKAMETFNHNNGNDVLYQPPTAS
  VSTSFLQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_459935
• RefSeq ORF: 1491
• MW: 57.1 kDa