nsp14 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100586

Myc-DDK-tagged ORF clone of viral ORF for nsp14 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460021

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100586 represents NCBI reference of YP_460021 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTACCACTAACCTCTTCAAGGACTGCTCCAAGAGCTGCCTGGGATATCATCCTGCACATGCACCTT
  CTTTTCTGGCTGTCGACGATAAGTATAAAGTTAACGAAAACCTGGCCGTGAACCTGAACATTTGTGAACC
  CGTCCTGACTTATTCAAGGTTGATTAGCTTGATGGGCTTCAAGCTGGACCTCACACTCGACGGCTACTCT
  AAACTCTTCATTACCAAGGACGAGGCCATCAAGCGGGTGAGGGGTTGGGTGGGCTTTGACGTCGAGGGGG
  CGCACGCGACACGAGAAAACATCGGAACTAACTTTCCACTCCAGATAGGTTTCTCTACCGGAGTGGATTT
  CGTGGTGGAGGCAACCGGTCTGTTTGCAGAAAGGGATTGTTACACGTTCAAAAAGACAGTCGCCAAGGCT
  CCCCCCGGTGAGAAGTTCAAGCACCTTATTCCACTTATGTCCAAGGGACAAAAGTGGGACATCGTGCGCA
  TCAGAATCGTTCAGATGCTGTCAGATTACCTGCTGGATTTGTCAGACTCCGTGGTCTTTATCACCTGGTC
  AGCCTCCTTCGAACTTACTTGCTTGCGGTACTTCGCCAAGCTCGGGCGAGAGCTGAACTGCAACGTATGC
  TCAAACCGCGCCACATGTTACAACAGCAGAACAGGCTACTATGGATGCTGGCGCCATAGCTATACCTGTG
  ATTACGTATATAACCCGCTGATAGTTGATATCCAGCAGTGGGGTTATACCGGAAGTCTGACCTCAAACCA
  CGATATCATCTGTAATGTCCACAAGGGCGCACACGTAGCGTCCGCTGATGCCATTATGACGAGGTGTTTG
  GCAATCTATGATTGCTTCTGCAAGAGCGTAAATTGGAACTTGGAATACCCAATTATCAGTAACGAGGTGA
  GCATCAATACCTCATGCCGCCTGCTGCAAAGAGTGATGCTGAAAGCTGCTATGCTCTGTAATAGGTATAA
  TTTGTGTTATGATATCGGAAACCCGAAAGGACTGGCCTGTGTTAAGGATTACGAGTTTAAGTTCTACGAT
  GCCTTCCCCGTCGCTAAATCCGTGAAGCAGCTTTTTTATGTCTACGACGTGCACAAAGATAATTTCAAGG
  ATGGGCTCTGCATGTTCTGGAACTGCAACGTCGATAAGTATCCTAGCAACAGCATCGTGTGTCGGTTCGA
  CACCCGGGTCCTGAATAAATTGAATCTGCCCGGATGTAACGGCGGTAGCCTGTATGTAAATAAACACGCA
  TTTCACACAAACCCATTTACCAGAACCGTCTTTGAGAATCTGAAGCCCATGCCATTTTTCTACTACTCCG
  ATACACCATGTGTTTACGTGGACGGGTTGGAGTCCAAGCAGGTGGATTATGTGCCACTGCGGTCAGCCAC
  TTGTATTACCCGATGTAACCTCGGAGGAGCTGTGTGTAGTAAGCACGCTGAGGAATACTGTAACTACCTG
  GAGAGCTATAACATCGTGACTACAGCCGGCTTTACCTTCTGGGTGTACAAGAACTTCGATTTTTACAATT
  TGTGGAACACGTTTACAACACTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100586 representing YP_460021
  Red=Cloning sites Green=Tags
  
  MCTTNLFKDCSKSCLGYHPAHAPSFLAVDDKYKVNENLAVNLNICEPVLTYSRLISLMGFKLDLTLDGYS
  KLFITKDEAIKRVRGWVGFDVEGAHATRENIGTNFPLQIGFSTGVDFVVEATGLFAERDCYTFKKTVAKA
  PPGEKFKHLIPLMSKGQKWDIVRIRIVQMLSDYLLDLSDSVVFITWSASFELTCLRYFAKLGRELNCNVC
  SNRATCYNSRTGYYGCWRHSYTCDYVYNPLIVDIQQWGYTGSLTSNHDIICNVHKGAHVASADAIMTRCL
  AIYDCFCKSVNWNLEYPIISNEVSINTSCRLLQRVMLKAAMLCNRYNLCYDIGNPKGLACVKDYEFKFYD
  AFPVAKSVKQLFYVYDVHKDNFKDGLCMFWNCNVDKYPSNSIVCRFDTRVLNKLNLPGCNGGSLYVNKHA
  FHTNPFTRTVFENLKPMPFFYYSDTPCVYVDGLESKQVDYVPLRSATCITRCNLGGAVCSKHAEEYCNYL
  ESYNIVTTAGFTFWVYKNFDFYNLWNTFTTLQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_460021
• RefSeq ORF: 1566
• MW: 59.7 kDa