nsp6 (hydrophobic domain) (NC_006577) Virus Tagged ORF Clone

CAT#: VC100579

Myc-DDK-tagged ORF clone of viral ORF for nsp6 (hydrophobic domain) [Human coronavirus HKU1], codon optimized for human cell expression, YP_460019

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp6 (hydrophobic domain)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100579 represents NCBI reference of YP_460019 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAGACTAAGAGATTTATCAAGGAAACAATTTATTGGATTCTGATCAGTACCTTTCTGTTCTCTT
  GTATCATTAGCGCCTTCGTGAAGTGGACAATATTCATGTATATCAATACTCATATGATCGGCGTGACCCT
  GTGTGTTCTCTGCTTCGTTTCTTTCATGATGCTGCTGGTTAAGCACAAGCACTTCTATCTGACCATGTAT
  ATAATCCCGGTGCTGTGCACATTGTTCTACGTTAACTATCTGGTAGTGTACAAGGAGGGCTTCCGCGGAT
  TCACATACGTCTGGTTGTCTTACTTTGTGCCCGCCGTCAACTTCACTTATGTCTATGAAGTGTTCTACGG
  ATGTATCCTGTGTGTTTTCGCCATCTTTATAACCATGCACAGCATCAATCATGATATCTTCTCCCTGATG
  TTCCTCGTTGGCAGGATCGTCACCCTGATTTCTATGTGGTATTTCGGTTCTAACCTGGAAGAAGACGTGC
  TGCTGTTCATAACTGCATTTCTCGGTACCTATACGTGGACGACCATACTGTCCCTGGCTATTGCCAAGAT
  CGTGGCAAACTGGCTGTCCGTTAACATTTTTTACTTCACAGATGTGCCCTACATCAAGCTCATTCTGCTG
  AGTTACCTCTTCATTGGGTATATTTTGTCTTGCTACTGGGGCTTTTTTTCCCTGTTGAACTCTGTGTTCA
  GGATGCCAATGGGGGTGTATAATTATAAGATTTCCGTGCAAGAGCTTAGGTACATGAACGCTAATGGTCT
  GAGGCCACCAAGGAACTCCTTTGAAGCCATCCTGCTTAATCTCAAACTGTTGGGAATTGGCGGCGTGCCA
  GTGATCGAGGTGAGCCAGATTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100579 representing YP_460019
  Red=Cloning sites Green=Tags
  
  MSKTKRFIKETIYWILISTFLFSCIISAFVKWTIFMYINTHMIGVTLCVLCFVSFMMLLVKHKHFYLTMY
  IIPVLCTLFYVNYLVVYKEGFRGFTYVWLSYFVPAVNFTYVYEVFYGCILCVFAIFITMHSINHDIFSLM
  FLVGRIVTLISMWYFGSNLEEDVLLFITAFLGTYTWTTILSLAIAKIVANWLSVNIFYFTDVPYIKLILL
  SYLFIGYILSCYWGFFSLLNSVFRMPMGVYNYKISVQELRYMNANGLRPPRNSFEAILLNLKLLGIGGVP
  VIEVSQIQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 864 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_460019
• RefSeq ORF: 864
• MW: 33.7 kDa