M (NC_006577) Virus Tagged ORF Clone

CAT#: VC100574

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173241

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: M
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100574 represents NCBI reference of YP_173241 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAGAGCTTCCTGCCCCAGTTCACCAGCGACCAGGCCGTGACCTTCCTGAAGGAGTGGAACTTCT
  CCCTGGGCGTGATTCTGCTGTTCATCACCATTATCCTGCAGTTCGGCTACACCAGCCGGAGCATGTTCGT
  GTACCTGATCAAGATGATCATCCTCTGGCTGATGTGGCCCCTGACCATCACCCTGACCATCTTCAATTGC
  TTCTACGCACTGAACAACGCCTTCCTGGCCTTCAGCATCGTCTTCACCATCATCAGCATCGTCATTTGGA
  TCCTCTACTTCGTGAACAGCATCCGGCTGTTTATCCGGACCGGCAGCTGGTGGAGCTTCAACCCCGAGAC
  CAACAATCTGATGTGCATCGACATGAAGGGCAAGATGTTCGTGCGGCCCGTGATCGAGGACTACCACACC
  CTGACAGCCACTGTGATCCGGGGCCACCTGTACATTCAGGGCGTGAAGCTGGGCACTGGCTACACACTGA
  GCGATCTGCCCGTGTACGTGACCGTGGCCAAAGTGCAAGTGCTGTGCACCTACAAGCGGGCCTTCCTGGA
  CAAGCTGGACGTGAACAGCGGCTTCGCCGTCTTCGTCAAGAGCAAGGTGGGCAACTACCGGCTGCCCTCC
  AGCAAACCCAGCGGCATGGATACTGCTCTCCTGCGGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100574 representing YP_173241
  Red=Cloning sites Green=Tags
  
  MNKSFLPQFTSDQAVTFLKEWNFSLGVILLFITIILQFGYTSRSMFVYLIKMIILWLMWPLTITLTIFNC
  FYALNNAFLAFSIVFTIISIVIWILYFVNSIRLFIRTGSWWSFNPETNNLMCIDMKGKMFVRPVIEDYHT
  LTATVIRGHLYIQGVKLGTGYTLSDLPVYVTVAKVQVLCTYKRAFLDKLDVNSGFAVFVKSKVGNYRLPS
  SKPSGMDTALLRA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 669 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_173241
• RefSeq ORF: 669
• Locus ID: 3200428
• MW: 25.6 kDa