nsp15 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100587

Myc-DDK-tagged ORF clone of viral ORF for nsp15 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460022

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100587 represents NCBI reference of YP_460022 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTTGAGAACGTGATCTACAACCTGGTGAATGTTGGACACTACGACGGGAGGACCGGCGAGCTGC
  CGTGTGCTATCATGAATGACAAAGTGGTGGTGAAAATCAATAACGTCGATACTGTTATTTTCAAGAACAA
  TACCTCCTTCCCAACTAACATAGCCGTGGAGCTGTTTACTAAAAGGAGTATACGGCACCACCCCGAGCTG
  AAGATACTCCGGAACTTGAACATAGACATCTGTTGGAAACACGTGCTGTGGGACTATGTCAAGGATTCTC
  TGTTTTGCAGCAGTACTTACGGAGTGTGCAAGTACACAGATCTTAAGTTCATCGAGAATCTGAACATCCT
  GTTTGATGGCAGAGATACCGGGGCTCTCGAGGCCTTTCGGAAGGCTAGGAATGGTGTCTTTATATCTACT
  GAGAAGCTGAGCCGGCTGAGCATGATTAAAGGACCCCAAAGAGCAGATCTGAACGGCGTCATCGTCGACA
  AAGTGGGCGAACTCAAAGTGGAATTCTGGTTTGCAATGAGAAAAGACGGAGATGACGTGATCTTCTCTCG
  AACCGACAGCCTGTGTTCTAGCCACTATTGGTCACCTCAGGGCAACCTTGGTGGCAACTGCGCCGGGAAT
  GTCATTGGTAATGACGCACTGACACGCTTTACCATATTCACCCAGTCACGGGTGCTCTCATCATTTGAAC
  CACGGTCAGACCTTGAAAGAGACTTCATCGATATGGATGACAATCTGTTCATTGCAAAATACGGCCTGGA
  GGACTATGCATTTGATCACATAGTTTATGGTAGCTTCAACCACAAAGTGATCGGAGGGCTTCATCTCTTG
  ATCGGCCTGTTCAGAAGAAAAAAGAAGAGTAACTTGCTGATACAGGAATTTCTGCAGTATGATTCAAGCA
  TCCACAGCTACTTTATCACGGATCAGGAGTGCGGATCCTCAAAGAGCGTCTGCACTGTCATCGATCTGCT
  GTTGGATGATTTTGTGTCCATTGTGAAAAGTTTGAACCTGAGCTGTGTGTCTAAGGTGGTGAACATAAAT
  GTGGACTTCAAGGACTTCCAGTTTATGCTTTGGTGCAATGATAATAAAATAATGACATTTTATCCCAAGA
  TGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100587 representing YP_460022
  Red=Cloning sites Green=Tags
  
  MSLENVIYNLVNVGHYDGRTGELPCAIMNDKVVVKINNVDTVIFKNNTSFPTNIAVELFTKRSIRHHPEL
  KILRNLNIDICWKHVLWDYVKDSLFCSSTYGVCKYTDLKFIENLNILFDGRDTGALEAFRKARNGVFIST
  EKLSRLSMIKGPQRADLNGVIVDKVGELKVEFWFAMRKDGDDVIFSRTDSLCSSHYWSPQGNLGGNCAGN
  VIGNDALTRFTIFTQSRVLSSFEPRSDLERDFIDMDDNLFIAKYGLEDYAFDHIVYGSFNHKVIGGLHLL
  IGLFRRKKKSNLLIQEFLQYDSSIHSYFITDQECGSSKSVCTVIDLLLDDFVSIVKSLNLSCVSKVVNIN
  VDFKDFQFMLWCNDNKIMTFYPKMQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_460022
• RefSeq ORF: 1125
• MW: 42.9 kDa