nsp7 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100581

Myc-DDK-tagged ORF clone of viral ORF for nsp7 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459938

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100581 represents NCBI reference of YP_459938 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAAGCTGACGGACGTCAAATGTGCCAACGTGGTTCTCCTGAATTGTCTGCAACACCTGCATGTGG
  CTTCCAACTCAAAGCTCTGGCAGTACTGCTCAGTGCTGCATAACGAGATCCTCAGCACGTCAGACCTCTC
  TGTAGCCTTCGACAAGCTGGCACAACTGCTCATCGTACTTTTCGCCAACCCGGCAGCCGTTGACACTAAA
  TGCCTGGCTAGCATCGATGAAGTGTCCGATGACTACGTGCAGGACTCTACCGTTCTCCAGGCTCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100581 representing YP_459938
  Red=Cloning sites Green=Tags
  
  MSKLTDVKCANVVLLNCLQHLHVASNSKLWQYCSVLHNEILSTSDLSVAFDKLAQLLIVLFANPAAVDTK
  CLASIDEVSDDYVQDSTVLQALQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 279 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_459938
• RefSeq ORF: 279
• MW: 10.2 kDa