nsp10 (NC_006577) Virus Tagged ORF Clone

CAT#: VC100584

Myc-DDK-tagged ORF clone of viral ORF for nsp10 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459939

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nsp10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100584 represents NCBI reference of YP_459939 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGAGTGGCTACTGAATATGCCGCCAACTCATCAATTCTCTCCCTCTGTGCCTTCTCCGTTGATC
  CTAAAAAGACGTACCTTGACTACATTCAGCAGGGTGGGGTGCCAATCATCAATTGCGTCAAAATGCTGTG
  CGATCATGCCGGGACAGGGATGGCTATTACTATCAAGCCGGAAGCAACTATAAATCAAGATTCCTACGGG
  GGCGCATCAGTGTGTATCTATTGTCGAGCGCGCGTGGAACACCCGGATGTGGATGGGATTTGCAAGTTGA
  GAGGGAAGTTTGTTCAGGTGCCTCTTGGCATCAAAGACCCAATACTCTACGTTTTGACTCACGACGTATG
  TCAGGTCTGCGGTTTCTGGCGGGATGGCTCTTGCTCCTGCGTGGGTTCCAGCGTCGCAGTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100584 representing YP_459939
  Red=Cloning sites Green=Tags
  
  MAGVATEYAANSSILSLCAFSVDPKKTYLDYIQQGGVPIINCVKMLCDHAGTGMAITIKPEATINQDSYG
  GASVCIYCRARVEHPDVDGICKLRGKFVQVPLGIKDPILYVLTHDVCQVCGFWRDGSCSCVGSSVAVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 414 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_459939
• RefSeq ORF: 414
• MW: 14.7 kDa