N (NC_006577) Virus Tagged ORF Clone

CAT#: VC100575

Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid phosphoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173242

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100575 represents NCBI reference of YP_173242 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACACCCCCGGCCATTACGCCGGCAGCCGGAGCTCCTCCGGAAATCGGTCCGGCATCCTCAAGA
  AGACCAGCTGGGCCGACCAGAGCGAGCGGAACTACCAGACCTTCAATAGAGGACGGAAGACCCAGCCCAA
  GTTCACCGTCTCCACCCAGCCCCAGGGGAACACAATTCCCCACTACAGCTGGTTCAGCGGGATCACCCAA
  TTTCAAAAAGGCCGGGACTTCAAGTTTAGCGACGGCCAGGGCGTGCCCATCGCCTTCGGCGTGCCTCCCA
  GCGAAGCCAAGGGGTACTGGTATCGGCATAGCCGGCGGAGCTTCAAGACCGCCGACGGGCAGCAGAAGCA
  GCTCCTGCCTCGGTGGTACTTCTACTACCTCGGCACAGGCCCCTACGCTAATGCCAGCTACGGCGAGAGC
  CTGGAGGGCGTGTTTTGGGTCGCAAACCACCAGGCCGACACCTCCACTCCCAGCGACGTGAGCTCCCGGG
  ACCCCACCACCCAGGAGGCCATCCCCACCCGGTTTCCCCCCGGCACCATCCTGCCCCAAGGCTACTACGT
  GGAGGGGTCCGGCCGGTCCGCCAGCAATTCCCGGCCCGGCAGCCGGTCCCAGAGCCGGGGACCCAACAAC
  CGGTCCCTGAGCCGGTCCAACAGCAACTTCCGGCACAGCGACAGCATCGTGAAGCCCGATATGGCAGACG
  AGATCGCCAACCTGGTGCTGGCCAAGCTCGGCAAGGACAGCAAGCCACAGCAGGTCACCAAGCAGAATGC
  CAAGGAAATCCGGCATAAGATTCTGACCAAGCCCCGGCAAAAGCGGACCCCAAACAAGCACTGCAACGTG
  CAGCAGTGCTTCGGCAAGCGGGGGCCCAGCCAAAACTTCGGGAACGCCGAGATGCTCAAGCTGGGCACCA
  ATGACCCCCAGTTCCCCATCCTCGCCGAGCTGGCCCCCACCCCCGGCGCCTTCTTCTTCGGCTCCAAGCT
  CGACCTGGTGAAGCGGGATAGCGAGGCCGACAGCCCCGTGAAGGACGTCTTTGAGCTGCATTACAGCGGG
  AGCATCCGGTTCGACAGCACCCTGCCCGGCTTCGAGACCATCATGAAGGTGCTGGAGGAGAACCTCAACG
  CCTACGTGAACAGCAACCAGAACACCGACTCCGACAGCCTGTCCAGCAAGCCCCAGAGAAAGCGGGGCGT
  GAAACAACTGCCCGAGCAGTTCGACAGCCTGAACCTGTCCGCCGGCACCCAGCACATCTCCAACGACTTC
  ACACCCGAAGATCACAGCCTCCTGGCCACCCTCGACGACCCATACGTCGAGGACAGCGTCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100575 representing YP_173242
  Red=Cloning sites Green=Tags
  
  MSYTPGHYAGSRSSSGNRSGILKKTSWADQSERNYQTFNRGRKTQPKFTVSTQPQGNTIPHYSWFSGITQ
  FQKGRDFKFSDGQGVPIAFGVPPSEAKGYWYRHSRRSFKTADGQQKQLLPRWYFYYLGTGPYANASYGES
  LEGVFWVANHQADTSTPSDVSSRDPTTQEAIPTRFPPGTILPQGYYVEGSGRSASNSRPGSRSQSRGPNN
  RSLSRSNSNFRHSDSIVKPDMADEIANLVLAKLGKDSKPQQVTKQNAKEIRHKILTKPRQKRTPNKHCNV
  QQCFGKRGPSQNFGNAEMLKLGTNDPQFPILAELAPTPGAFFFGSKLDLVKRDSEADSPVKDVFELHYSG
  SIRFDSTLPGFETIMKVLEENLNAYVNSNQNTDSDSLSSKPQRKRGVKQLPEQFDSLNLSAGTQHISNDF
  TPEDHSLLATLDDPYVEDSVA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006577
• ORF Size: 1323 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006577.2, YP_173242
• RefSeq ORF: 1323
• Locus ID: 3200423
• MW: 49.1 kDa