N (NC_006577) Virus Tagged ORF Clone

CAT#: VC100575

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid phosphoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173242


  View other clones from "HCoV-HKU1" (18)

Reconstitution Protocol

USD 570.00

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Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol N
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100575 represents NCBI reference of YP_173242 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTACACCCCCGGCCATTACGCCGGCAGCCGGAGCTCCTCCGGAAATCGGTCCGGCATCCTCAAGA
AGACCAGCTGGGCCGACCAGAGCGAGCGGAACTACCAGACCTTCAATAGAGGACGGAAGACCCAGCCCAA
GTTCACCGTCTCCACCCAGCCCCAGGGGAACACAATTCCCCACTACAGCTGGTTCAGCGGGATCACCCAA
TTTCAAAAAGGCCGGGACTTCAAGTTTAGCGACGGCCAGGGCGTGCCCATCGCCTTCGGCGTGCCTCCCA
GCGAAGCCAAGGGGTACTGGTATCGGCATAGCCGGCGGAGCTTCAAGACCGCCGACGGGCAGCAGAAGCA
GCTCCTGCCTCGGTGGTACTTCTACTACCTCGGCACAGGCCCCTACGCTAATGCCAGCTACGGCGAGAGC
CTGGAGGGCGTGTTTTGGGTCGCAAACCACCAGGCCGACACCTCCACTCCCAGCGACGTGAGCTCCCGGG
ACCCCACCACCCAGGAGGCCATCCCCACCCGGTTTCCCCCCGGCACCATCCTGCCCCAAGGCTACTACGT
GGAGGGGTCCGGCCGGTCCGCCAGCAATTCCCGGCCCGGCAGCCGGTCCCAGAGCCGGGGACCCAACAAC
CGGTCCCTGAGCCGGTCCAACAGCAACTTCCGGCACAGCGACAGCATCGTGAAGCCCGATATGGCAGACG
AGATCGCCAACCTGGTGCTGGCCAAGCTCGGCAAGGACAGCAAGCCACAGCAGGTCACCAAGCAGAATGC
CAAGGAAATCCGGCATAAGATTCTGACCAAGCCCCGGCAAAAGCGGACCCCAAACAAGCACTGCAACGTG
CAGCAGTGCTTCGGCAAGCGGGGGCCCAGCCAAAACTTCGGGAACGCCGAGATGCTCAAGCTGGGCACCA
ATGACCCCCAGTTCCCCATCCTCGCCGAGCTGGCCCCCACCCCCGGCGCCTTCTTCTTCGGCTCCAAGCT
CGACCTGGTGAAGCGGGATAGCGAGGCCGACAGCCCCGTGAAGGACGTCTTTGAGCTGCATTACAGCGGG
AGCATCCGGTTCGACAGCACCCTGCCCGGCTTCGAGACCATCATGAAGGTGCTGGAGGAGAACCTCAACG
CCTACGTGAACAGCAACCAGAACACCGACTCCGACAGCCTGTCCAGCAAGCCCCAGAGAAAGCGGGGCGT
GAAACAACTGCCCGAGCAGTTCGACAGCCTGAACCTGTCCGCCGGCACCCAGCACATCTCCAACGACTTC
ACACCCGAAGATCACAGCCTCCTGGCCACCCTCGACGACCCATACGTCGAGGACAGCGTCGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100575 representing YP_173242
Red=Cloning sites Green=Tags

MSYTPGHYAGSRSSSGNRSGILKKTSWADQSERNYQTFNRGRKTQPKFTVSTQPQGNTIPHYSWFSGITQ
FQKGRDFKFSDGQGVPIAFGVPPSEAKGYWYRHSRRSFKTADGQQKQLLPRWYFYYLGTGPYANASYGES
LEGVFWVANHQADTSTPSDVSSRDPTTQEAIPTRFPPGTILPQGYYVEGSGRSASNSRPGSRSQSRGPNN
RSLSRSNSNFRHSDSIVKPDMADEIANLVLAKLGKDSKPQQVTKQNAKEIRHKILTKPRQKRTPNKHCNV
QQCFGKRGPSQNFGNAEMLKLGTNDPQFPILAELAPTPGAFFFGSKLDLVKRDSEADSPVKDVFELHYSG
SIRFDSTLPGFETIMKVLEENLNAYVNSNQNTDSDSLSSKPQRKRGVKQLPEQFDSLNLSAGTQHISNDF
TPEDHSLLATLDDPYVEDSVA

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006577
ORF Size 1323 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_006577.2, YP_173242
RefSeq ORF 1323
Locus ID 3200423
MW 49.1 kDa

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