UL5 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100869

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase helicase subunit [Human herpesvirus 2], codon optimized for human cell expression, NP_044474

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100869 represents NCBI reference of NP_044474 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCTCCGGAGGCGAGGGCAGCAGGGACGTAAGGGCTCCCGGACCTCCGCCTCAACAGCCAGGTG
  CCAGACCTGCAGTGAGATTCAGAGATGAGGCTTTTTTGAACTTTACCTCCATGCACGGAGTCCAGCCCAT
  AATCGCAAGAATCAGGGAGCTGTCCCAGCAGCAGCTGGATGTGACTCAAGTCCCGCGGTTGCAATGGTTC
  CGCGACGTTGCCGCCCTGGAGGTGCCAACAGGACTGCCTTTGCGCGAGTTCCCGTTTGCCGCTTATCTGA
  TAACAGGAAACGCTGGGAGTGGGAAATCCACCTGCGTGCAAACCCTGAACGAAGTCCTGGACTGCGTGGT
  GACCGGAGCGACTCGCATTGCAGCACAGAACATGTACGTCAAACTTAGTGGCGCATTCCTCAGTAGGCCG
  ATTAATACCATCTTCCACGAGTTCGGGTTTCGGGGGAACCACGTTCAAGCACAGCTGGGACAACACCCAT
  ATACACTGGCCTCCTCCCCCGCTTCCCTCGAAGATTTGCAGAGACGGGACCTGACATACTACTGGGAGGT
  GATCCTTGACATCACTAAGAGAGCCCTGGCCGCCCACGGGGGAGAAGACGCTAGAAATGAGTTCCATGCG
  CTCACCGCCCTGGAACAGACCCTGGGATTGGGACAGGGAGCCCTTACCCGCCTGGCATCTGTGACTCACG
  GAGCTCTGCCGGCTTTCACAAGAAGCAACATTATAGTTATAGATGAGGCCGGTCTGCTGGGGAGGCACCT
  CCTGACTACAGTAGTGTACTGTTGGTGGATGATTAACGCTCTGTACCACACACCACAGTACGCCGGTAGA
  TTGCGCCCTGTTCTGGTATGTGTCGGGTCTCCGACACAGACAGCCTCCCTTGAGTCTACATTCGAGCATC
  AGAAGCTGAGATGTTCAGTGAGACAGTCTGAAAATGTGCTGACATATCTCATTTGTAACAGGACACTGCG
  AGAATATACCCGACTTTCTCACTCCTGGGCCATATTCATCAATAATAAAAGATGCGTGGAGCATGAGTTC
  GGGAATTTGATGAAAGTTCTGGAGTACGGGTTGCCAATCACAGAAGAGCACATGCAATTCGTAGACCGGT
  TTGTGGTGCCTGAATCTTACATAACCAACCCAGCCAACCTGCCTGGCTGGACCAGACTTTTCTCCAGTCA
  TAAAGAAGTGTCAGCCTATATGGCTAAACTGCACGCCTATCTCAAGGTGACGAGAGAGGGGGAATTCGTT
  GTGTTCACTCTGCCAGTTCTCACATTTGTTTCCGTCAAGGAGTTCGATGAGTACAGGCGCCTGACCCAGC
  AGCCAACCCTTACTATGGAAAAGTGGATTACTGCCAACGCTTCTCGGATCACTAATTATAGCCAGTCACA
  GGACCAAGATGCAGGACACGTACGCTGTGAAGTTCACTCAAAACAACAGCTGGTAGTGGCGAGAAATGAT
  ATCACCTACGTTCTGAACTCCCAGGTGGCCGTAACTGCACGCCTGAGAAAGATGGTGTTCGGCTTTGATG
  GAACTTTCAGAACTTTCGAGGCCGTATTGCGGGACGACAGTTTCGTGAAGACCCAGGGGGAAACCTCAGT
  GGAATTCGCCTATCGGTTTCTGTCCCGGCTGATGTTCGGAGGTCTGATACACTTTTACAACTTTCTCCAA
  CGACCAGGCTTGGACGCCACCCAGAGAACCCTGGCTTATGGGCGGTTGGGTGAACTTACAGCTGAACTGC
  TGAGCCTGCGCAGAGACGCCGCCGGGGCCTCCGCAACTCGAGCTGCTGATACAAGCGACCGGAGTCCCGG
  AGAGAGAGCTTTCAACTTTAAACATTTGGGACCCAGGGACGGAGGTCCGGACGATTTTCCCGACGACGAC
  CTGGACGTCATTTTTGCCGGCCTGGACGAACAGCAGTTGGACGTGTTCTATTGTCATTACGCCCTGGAGG
  AACCGGAGACCACCGCAGCTGTCCATGCCCAGTTCGGTCTGCTTAAGAGGGCGTTTCTGGGACGGTATCT
  GATCCTGCGGGAGCTGTTCGGAGAAGTGTTTGAATCTGCGCCTTTCAGTACATACGTGGATAACGTAATC
  TTCAGGGGCTGCGAGCTCCTGACCGGTAGCCCACGCGGAGGTCTGATGAGCGTGGCGTTGCAGACAGACA
  ACTACACTTTGATGGGCTATACATACACTAGGGTCTTCGCATTTGCCGAAGAGCTGAGAAGGCGGCACGC
  TACTGCAGGCGTCGCCGAATTTCTCGAAGAGAGCCCTCTGCCATACATAGTGCTGCGAGACCAACACGGC
  TTCATGTCCGTGGTTAACACAAATATCAGCGAGTTCGTAGAGTCAATCGACAGTACAGAACTGGCTATGG
  CAATTAATGCTGATTACGGCATCTCTTCTAAACTGGCTATGACCATTACAAGATCTCAAGGATTGTCTCT
  GGACAAGGTCGCTATTTGTTTTACACCCGGGAATCTTCGACTGAACAGCGCATACGTGGCTATGAGTAGG
  ACAACCAGCTCCGAGTTTCTGCACATGAATCTGAACCCCCTGCGAGAGAGACACGAGAGGGACGATGTGA
  TAAGTGAACATATACTTTCAGCTCTGAGAGACCCGAATGTGGTAATCGTCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100869 representing NP_044474
  Red=Cloning sites Green=Tags
  
  MAASGGEGSRDVRAPGPPPQQPGARPAVRFRDEAFLNFTSMHGVQPIIARIRELSQQQLDVTQVPRLQWF
  RDVAALEVPTGLPLREFPFAAYLITGNAGSGKSTCVQTLNEVLDCVVTGATRIAAQNMYVKLSGAFLSRP
  INTIFHEFGFRGNHVQAQLGQHPYTLASSPASLEDLQRRDLTYYWEVILDITKRALAAHGGEDARNEFHA
  LTALEQTLGLGQGALTRLASVTHGALPAFTRSNIIVIDEAGLLGRHLLTTVVYCWWMINALYHTPQYAGR
  LRPVLVCVGSPTQTASLESTFEHQKLRCSVRQSENVLTYLICNRTLREYTRLSHSWAIFINNKRCVEHEF
  GNLMKVLEYGLPITEEHMQFVDRFVVPESYITNPANLPGWTRLFSSHKEVSAYMAKLHAYLKVTREGEFV
  VFTLPVLTFVSVKEFDEYRRLTQQPTLTMEKWITANASRITNYSQSQDQDAGHVRCEVHSKQQLVVARND
  ITYVLNSQVAVTARLRKMVFGFDGTFRTFEAVLRDDSFVKTQGETSVEFAYRFLSRLMFGGLIHFYNFLQ
  RPGLDATQRTLAYGRLGELTAELLSLRRDAAGASATRAADTSDRSPGERAFNFKHLGPRDGGPDDFPDDD
  LDVIFAGLDEQQLDVFYCHYALEEPETTAAVHAQFGLLKRAFLGRYLILRELFGEVFESAPFSTYVDNVI
  FRGCELLTGSPRGGLMSVALQTDNYTLMGYTYTRVFAFAEELRRRHATAGVAEFLEESPLPYIVLRDQHG
  FMSVVNTNISEFVESIDSTELAMAINADYGISSKLAMTITRSQGLSLDKVAICFTPGNLRLNSAYVAMSR
  TTSSEFLHMNLNPLRERHERDDVISEHILSALRDPNVVIVY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 2643 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044474
• RefSeq ORF: 2643
• Locus ID: 1487338
• MW: 98.9 kDa