UL27 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100892

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein B [Human herpesvirus 2], codon optimized for human cell expression, NP_044497

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100892 represents NCBI reference of NP_044497 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGAGGGGGACTCATCTGCGCGTTGGTCGTTGGGGCCTTGGTGGCCGCTGTTGCCTCAGCCGCCC
  CAGCAGCTCCAGCAGCACCCAGAGCTTCTGGAGGAGTCGCTGCCACTGTCGCTGCCAATGGAGGACCCGC
  TTCAAGACCACCTCCTGTGCCATCACCAGCAACCACAAAGGCCAGAAAGAGGAAGACGAAAAAGCCGCCA
  AAACGCCCCGAGGCCACTCCCCCACCTGACGCAAACGCAACTGTGGCTGCAGGGCACGCAACCCTGCGCG
  CCCACCTGAGGGAAATCAAGGTAGAGAATGCTGACGCCCAGTTCTACGTTTGCCCACCCCCTACTGGAGC
  CACAGTCGTTCAGTTCGAGCAGCCTCGCAGGTGCCCAACTCGACCTGAAGGACAGAATTACACCGAGGGC
  ATCGCCGTAGTCTTCAAAGAGAACATTGCCCCGTATAAGTTCAAGGCAACAATGTATTACAAAGACGTGA
  CAGTGTCTCAAGTCTGGTTTGGCCACCGGTACTCCCAGTTTATGGGCATATTCGAAGATCGAGCCCCTGT
  ACCTTTCGAAGAGGTGATCGACAAAATCAACACCAAAGGCGTGTGCAGATCCACTGCGAAGTACGTCAGA
  AACAATATGGAAACCACAGCCTTCCATCGCGATGACCACGAAACAGACATGGAACTGAAACCAGCAAAAG
  TGGCAACGAGGACCAGCCGAGGGTGGCATACAACGGATTTGAAATATAACCCCAGCAGAGTCGAAGCTTT
  TCATAGATACGGGACTACTGTCAATTGTATTGTCGAGGAAGTGGATGCCAGAAGTGTCTATCCTTACGAT
  GAGTTTGTGCTTGCAACTGGAGATTTTGTTTATATGTCCCCATTTTACGGGTATCGCGAAGGATCCCACA
  CCGAACATACTAGCTACGCCGCTGACCGGTTTAAACAAGTGGACGGATTCTATGCTCGGGACCTGACTAC
  GAAAGCTCGCGCTACCAGCCCTACCACAAGAAATTTGCTTACCACGCCCAAGTTCACTGTTGCTTGGGAT
  TGGGTTCCCAAACGACCTGCAGTGTGCACAATGACCAAGTGGCAGGAGGTCGACGAGATGCTGCGCGCCG
  AGTACGGGGGATCTTTCCGGTTCTCTTCAGACGCAATTTCCACGACGTTTACCACTAACCTGACAGAGTA
  CTCACTGAGCAGGGTGGATCTTGGCGACTGTATTGGGAGAGATGCGCGCGAGGCTATTGACCGCATGTTC
  GCCCGCAAATATAACGCCACCCACATAAAGGTTGGGCAGCCACAATATTACTTGGCCACAGGCGGGTTTC
  TGATTGCTTACCAACCTCTGCTGTCCAACACCCTGGCAGAGTTGTACGTAAGGGAGTACATGAGGGAACA
  GGACCGGAAACCACGCAACGCTACCCCAGCTCCACTCCGCGAGGCACCCTCTGCCAATGCATCTGTAGAA
  CGCATTAAAACAACCAGCTCCATTGAATTCGCGCGGCTTCAGTTCACCTACAATCACATCCAAAGACACG
  TGAACGACATGCTTGGCAGGATCGCGGTCGCCTGGTGCGAGCTGCAGAACCACGAGCTGACTCTCTGGAA
  TGAAGCGAGGAAACTCAACCCGAACGCCATAGCCTCCGCGACTGTGGGAAGGCGAGTGTCAGCCAGGATG
  CTGGGCGACGTGATGGCCGTGTCTACTTGCGTCCCTGTGGCCCCTGACAACGTTATCGTACAAAATAGCA
  TGAGGGTGTCTAGCAGACCAGGCACTTGCTACTCTCGACCCCTCGTGAGCTTCAGGTATGAGGATCAGGG
  GCCATTGATAGAGGGGCAACTCGGCGAGAACAACGAGCTGAGACTGACGAGAGATGCTCTGGAGCCCTGC
  ACAGTCGGGCACCGCAGATATTTTATTTTTGGCGGGGGGTATGTCTACTTCGAGGAATACGCTTATAGCC
  ACCAGCTTAGCCGCGCAGACGTCACCACCGTGTCTACCTTTATCGATCTGAATATAACTATGCTGGAGGA
  TCACGAATTCGTGCCTCTGGAAGTGTATACCAGACATGAGATCAAAGACAGCGGGCTCCTTGACTATACC
  GAAGTGCAGAGACGGAACCAGCTCCACGACCTGCGGTTTGCAGATATAGATACCGTGATCCGCGCTGATG
  CTAATGCGGCTATGTTTGCAGGACTGTGCGCTTTTTTTGAAGGGATGGGTGACCTTGGCCGCGCAGTTGG
  CAAGGTCGTGATGGGGGTAGTCGGCGGGGTGGTCTCAGCTGTAAGTGGAGTCAGTTCTTTTATGAGCAAC
  CCATTTGGCGCTCTCGCCGTAGGTCTGCTTGTGCTCGCTGGCCTGGTCGCAGCCTTTTTTGCGTTTCGCT
  ATGTCCTGCAGTTGCAGCGAAATCCTATGAAGGCACTTTACCCCCTGACCACAAAGGAATTGAAGACCTC
  TGACCCTGGAGGAGTAGGGGGCGAGGGCGAGGAAGGCGCTGAGGGCGGCGGGTTTGATGAGGCTAAGCTG
  GCCGAAGCAAGAGAGATGATAAGATATATGGCACTGGTAAGCGCTATGGAGAGGACAGAACATAAGGCAC
  GAAAGAAAGGGACCAGCGCTCTTTTGAGTTCCAAAGTGACCAACATGGTCCTGCGCAAAAGAAATAAGGC
  GCGGTATAGCCCGCTCCACAACGAGGACGAGGCAGGAGATGAAGATGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100892 representing NP_044497
  Red=Cloning sites Green=Tags
  
  MRGGGLICALVVGALVAAVASAAPAAPAAPRASGGVAATVAANGGPASRPPPVPSPATTKARKRKTKKPP
  KRPEATPPPDANATVAAGHATLRAHLREIKVENADAQFYVCPPPTGATVVQFEQPRRCPTRPEGQNYTEG
  IAVVFKENIAPYKFKATMYYKDVTVSQVWFGHRYSQFMGIFEDRAPVPFEEVIDKINTKGVCRSTAKYVR
  NNMETTAFHRDDHETDMELKPAKVATRTSRGWHTTDLKYNPSRVEAFHRYGTTVNCIVEEVDARSVYPYD
  EFVLATGDFVYMSPFYGYREGSHTEHTSYAADRFKQVDGFYARDLTTKARATSPTTRNLLTTPKFTVAWD
  WVPKRPAVCTMTKWQEVDEMLRAEYGGSFRFSSDAISTTFTTNLTEYSLSRVDLGDCIGRDAREAIDRMF
  ARKYNATHIKVGQPQYYLATGGFLIAYQPLLSNTLAELYVREYMREQDRKPRNATPAPLREAPSANASVE
  RIKTTSSIEFARLQFTYNHIQRHVNDMLGRIAVAWCELQNHELTLWNEARKLNPNAIASATVGRRVSARM
  LGDVMAVSTCVPVAPDNVIVQNSMRVSSRPGTCYSRPLVSFRYEDQGPLIEGQLGENNELRLTRDALEPC
  TVGHRRYFIFGGGYVYFEEYAYSHQLSRADVTTVSTFIDLNITMLEDHEFVPLEVYTRHEIKDSGLLDYT
  EVQRRNQLHDLRFADIDTVIRADANAAMFAGLCAFFEGMGDLGRAVGKVVMGVVGGVVSAVSGVSSFMSN
  PFGALAVGLLVLAGLVAAFFAFRYVLQLQRNPMKALYPLTTKELKTSDPGGVGGEGEEGAEGGGFDEAKL
  AEAREMIRYMALVSAMERTEHKARKKGTSALLSSKVTNMVLRKRNKARYSPLHNEDEAGDEDEL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 2712 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044497
• RefSeq ORF: 2712
• Locus ID: 1487312
• MW: 100.2 kDa