US7 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100932

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein I [Human herpesvirus 2], codon optimized for human cell expression, NP_044537

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100932 represents NCBI reference of NP_044537 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGTCGCTCCTTGCAGGGGTTGGCGATCCTCGGGCTGTGGGTTTGCGCCACAGGACTGGTGGTTC
  GGGGGCCTACAGTAAGCCTGGTCAGTGATAGCCTTGTAGATGCTGGCGCTGTGGGTCCCCAAGGCTTTGT
  GGAGGAGGATCTGAGGGTGTTCGGGGAACTGCACTTTGTGGGAGCCCAAGTGCCACACACAAACTATTAT
  GACGGCATCATTGAGCTGTTCCACTACCCACTTGGGAATCATTGCCCTCGCGTCGTTCACGTGGTGACAC
  TGACAGCATGCCCTAGACGCCCCGCCGTTGCTTTTACCCTGTGTCGGTCCACCCATCACGCCCACTCACC
  GGCATATCCCACGCTCGAACTTGGCCTTGCGAGACAGCCACTCTTGCGCGTAAGAACCGCCACCAGGGAT
  TACGCCGGCCTGTATGTGCTGCGGGTTTGGGTTGGCTCCGCAACCAACGCCAGCCGCTTTGTCTTGGGCG
  TGGCCCTTTCCGCCAACGGCACCTTTGTGTATAATGGCTCCGATTATGGCTCATGTGATCCTGCTCAACT
  GCCCTTCTCTGCACCCCGCCTCGGCCCTTCCTCCGTGTACACACCGGGTGCCAGTCGCCCAACTCCCCCC
  CGGACCACTACACCACCATCTAGCCCTCGAGATCCTACACCTGCCCCTGGTGATACCGGGACTCCCGCCC
  CTGCCAGCGGGGAAATTGCACCTCCCAACTCCACTCGCAGCGCTAGCGAGAGCCGCCATCGCCTGACGGT
  CGCCCAAGTCATACAAATAGCTATCCCCGCTTCTATAATCGCCTTTGTGTTTCTGGGTAGCTGTATCTGC
  TTTATCCACCGCTGTCAGCGCCGATATAGACGCCCAAGGGGCCAGATATACAACCCAGGGGGAGTTAGCT
  GTGCCGTCAATGAAGCCGCGATGGCTCGCTTGGGCGCAGAGTTGAGGAGCCACCCAAACACGCCCCCCAA
  GCCGCGGAGACGCTCTAGCAGCTCAACCACCATGCCCAGTCTGACGTCAATTGCCGAAGAGAGCGAGCCT
  GGCCCAGTCGTGCTCCTGTCCGTCTCACCCAGACCCAGATCAGGCCCAACAGCCCCTCAGGAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100932 representing NP_044537
  Red=Cloning sites Green=Tags
  
  MPGRSLQGLAILGLWVCATGLVVRGPTVSLVSDSLVDAGAVGPQGFVEEDLRVFGELHFVGAQVPHTNYY
  DGIIELFHYPLGNHCPRVVHVVTLTACPRRPAVAFTLCRSTHHAHSPAYPTLELGLARQPLLRVRTATRD
  YAGLYVLRVWVGSATNASRFVLGVALSANGTFVYNGSDYGSCDPAQLPFSAPRLGPSSVYTPGASRPTPP
  RTTTPPSSPRDPTPAPGDTGTPAPASGEIAPPNSTRSASESRHRLTVAQVIQIAIPASIIAFVFLGSCIC
  FIHRCQRRYRRPRGQIYNPGGVSCAVNEAAMARLGAELRSHPNTPPKPRRRSSSSTTMPSLTSIAEESEP
  GPVVLLSVSPRPRSGPTAPQEV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044537
• RefSeq ORF: 1116
• Locus ID: 1487359
• MW: 39.6 kDa