UL46 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100911

Myc-DDK-tagged ORF clone of viral ORF for tegument protein VP11/12 [Human herpesvirus 2], codon optimized for human cell expression, NP_044516

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL46
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100911 represents NCBI reference of NP_044516 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGGCGGGCCAGAGGCGCTTCCAGCCTGAGACTCGCTAGATGCCTCACCCCTGCCAACCTGATCA
  GAGGGGCAAATGCCGGGGTCCCCGAACGGAGAATTTTCGCAGGGTGCCTCCTCCCTACTCCCGAAGGGCT
  CCTCTCCGCTGCAGTGGGCGTGCTCCGGCAGCGGGCCGATGACCTGCAGCCAGCCTTCCTCACCGGCGCT
  GACAGAAGCGTGCGGCTCGCTGCCCGGCATCACAACACCGTCCCTGAGTCCCTGATCGTGGACGGCCTCG
  CCAGCGATCCCCACTATGACTACATCCGGCACTACGCCAGCGCCGCCAAGCAGGCACTCGGCGAGGTCGA
  GCTGAGCGGCGGCCAACTGAGCCGGGCAATTCTCGCCCAGTACTGGAAATACCTCCAGACCGTCGTGCCC
  AGCGGGCTCGACATTCCTGACGACCCCGCCGGCGACTGCGACCCCTCCCTGCACGTGCTGCTCCGGCCCA
  CCCTGCTGCCCAAGCTCCTGGTGCGGGCCCCCTTCAAGTCCGGGGCCGCCGCCGCCAAGTACGCCGCCGC
  CGTGGCCGGCCTGCGGGACGCCGCCCACCGGCTGCAGCAATATATGTTCTTCATGCGGCCCGCCGACCCA
  TCCCGGCCCAGCACCGACACCGCCCTGCGGCTGTCCGAGCTCCTCGCATACGTGAGCGTCCTCTACCACT
  GGGCCAGCTGGATGCTGTGGACCGCCGACAAGTACGTGTGTCGGCGGCTCGGCCCTGCCGACCGGCGGTT
  CGTCGCCCTGTCCGGCAGCCTGGAGGCCCCCGCCGAAACCTTCGCCCGGCACCTCGACCGGGGGCCCTCC
  GGGACCACCGGGAGCATGCAGTGCATGGCCCTCCGGGCCGCCGTGAGCGATGTGCTGGGCCACCTCACCC
  GGCTCGCCCACCTCTGGGAAACCGGCAAGCGGAGCGGGGGCACCTACGGCATCGTGGACGCTATCGTCAG
  CACCGTCGAGGTCCTCTCCATCGTGCACCACCACGCCCAGTACATCATCAACGCAACCCTGACCGGGTAC
  GTGGTGTGGGCAAGCGACAGCCTGAACAACGAGTATCTGACTGCCGCCGTGGACAGCCAGGAACGGTTCT
  GCCGGACCGCTGCCCCCCTCTTCCCCACCATGACCGCCCCCTCCTGGGCACGGATGGAGCTGAGCATCAA
  GAGCTGGTTCGGCGCCGCCCTCGCCCCCGACCTCCTGCGGTCCGGCACACCCAGCCCCCACTATGAGAGC
  ATCCTCCGGCTCGCCGCAAGCGGCCCTCCCGGCGGCCGGGGCGCCGTGGGCGGCTCCTGTCGGGATAAAA
  TCCAGCGGACCCGGCGGGACAACGCCCCTCCTCCACTGCCCCGGGCCCGGCCTCACTCCACACCTGCCGC
  CCCCCGGCGGTGCAGACGGCACCGGGAGGACCTGCCCGAGCCCCCCCACGTCGACGCAGCCGATCGGGGC
  CCCGAGCCCTGCGCCGGGCGGCCCGCCACCTACTACACCCACATGGCCGGCGCCCCCCCCAGACTGCCAC
  CCCGGAACCCCGCTCCCCCCGAGCAGCGGCCCGCTGCCGCAGCCCGGCCCCTGGCCGCCCAGCGGGAAGC
  CGCAGGCGTCTACGACGCTGTGCGGACCTGGGGCCCCGACGCCGAGGCTGAGCCCGACCAAATGGAGAAC
  ACCTACCTGCTGCCCGACGACGACGCTGCAATGCCCGCAGGCGTGGGCCTGGGAGCCACACCCGCTGCCG
  ACACCACCGCCGCCGCCGCCTGGCCCGCAGAGAGCCACGCCCCCCGGGCACCCTCCGAGGACGCCGACAG
  CATTTACGAAAGCGTGGGCGAGGACGGCGGACGGGTGTACGAGGAGATCCCCTGGGTGCGGGTCTACGAG
  AACATTTGCCCCCGGCGGCGGCTGGCCGGGGGCGCCGCACTGCCTGGCGACGCTCCCGACTCCCCATACA
  TCGAGGCCGAGAACCCCCTGTACGACTGGGGCGGCAGCGCCCTCTTTAGCCCACGGCGGGCCACCCGGGC
  TCCAGACCCCGGGCTCAGCCTCTCCCCCATGCCCGCTCGGCCTCGGACAAACGCTCTGGCAAACGACGGC
  CCCACAAACGTGGCCGCTCTGTCCGCTCTCCTGACCAAACTCAAGCGGGGCCGGCACCAGTCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100911 representing NP_044516
  Red=Cloning sites Green=Tags
  
  MQRRARGASSLRLARCLTPANLIRGANAGVPERRIFAGCLLPTPEGLLSAAVGVLRQRADDLQPAFLTGA
  DRSVRLAARHHNTVPESLIVDGLASDPHYDYIRHYASAAKQALGEVELSGGQLSRAILAQYWKYLQTVVP
  SGLDIPDDPAGDCDPSLHVLLRPTLLPKLLVRAPFKSGAAAAKYAAAVAGLRDAAHRLQQYMFFMRPADP
  SRPSTDTALRLSELLAYVSVLYHWASWMLWTADKYVCRRLGPADRRFVALSGSLEAPAETFARHLDRGPS
  GTTGSMQCMALRAAVSDVLGHLTRLAHLWETGKRSGGTYGIVDAIVSTVEVLSIVHHHAQYIINATLTGY
  VVWASDSLNNEYLTAAVDSQERFCRTAAPLFPTMTAPSWARMELSIKSWFGAALAPDLLRSGTPSPHYES
  ILRLAASGPPGGRGAVGGSCRDKIQRTRRDNAPPPLPRARPHSTPAAPRRCRRHREDLPEPPHVDAADRG
  PEPCAGRPATYYTHMAGAPPRLPPRNPAPPEQRPAAAARPLAAQREAAGVYDAVRTWGPDAEAEPDQMEN
  TYLLPDDDAAMPAGVGLGATPAADTTAAAAWPAESHAPRAPSEDADSIYESVGEDGGRVYEEIPWVRVYE
  NICPRRRLAGGAALPGDAPDSPYIEAENPLYDWGGSALFSPRRATRAPDPGLSLSPMPARPRTNALANDG
  PTNVAALSALLTKLKRGRHQSH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 2166 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044516
• RefSeq ORF: 2166
• Locus ID: 1487333
• MW: 77.7 kDa