UL29 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100894

Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human herpesvirus 2], codon optimized for human cell expression, NP_044499

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL29
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100894 represents NCBI reference of NP_044499 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACGAAGCCTAAGACCACTACCACAGTAAAAGTGCCTCCTGGGCCGATGGGCTACGTGTATGGTA
  GGGCCTGTCCCGCAGAGGGCCTGGAGCTGCTTTCTCTGCTGTCCGCCAGGAGTGGCGATGCCGATGTTGC
  TGTCGCCCCCCTGATTGTAGGCCTCACCGTGGAGTCCGGGTTTGAGGCCAATGTGGCTGCCGTCGTGGGG
  AGCAGAACTACCGGTCTCGGAGGGACGGCAGTCTCTCTCAAGTTGATGCCTAGTCATTACAGCCCCTCAG
  TGTACGTTTTCCACGGCGGTCGGCACCTTGCTCCTTCTACCCAGGCTCCAAATCTCACCCGCCTCTGTGA
  GAGAGCCAGACCACATTTCGGGTTTGCCGACTATGCTCCACGGCCATGCGATCTTAAGCACGAAACAACC
  GGAGACGCACTGTGCGAGAGACTGGGCCTTGACCCTGACCGAGCCCTCCTGTACCTCGTGATCACTGAAG
  GCTTCAGAGAAGCCGTATGTATCAGCAACACCTTCCTGCATCTTGGTGGCATGGATAAAGTCACCATAGG
  CGACGCAGAAGTGCACAGGATCCCCGTATATCCTCTGCAAATGTTTATGCCTGATTTTTCTCGGGTAATC
  GCCGACCCATTCAACTGTAACCATCGGAGCATCGGGGAGAATTTTAATTATCCTCTGCCCTTTTTCAATC
  GCCCTCTTGCAAGGCTTCTCTTCGAAGCCGTCGTGGGTCCGGCCGCTGTTGCACTTAGAGCCAGGAACGT
  CGACGCCGTGGCAAGAGCAGCTGCACACCTGGCGTTTGATGAGAACCATGAGGGCGCTGCTCTTCCTGCT
  GATATTACATTCACAGCATTCGAAGCCTCCCAGGGAAAGCCTCAGAGAGGGGCTAGAGACGCTGGCAACA
  AGGGTCCAGCGGGGGGATTCGAGCAACGGCTCGCCTCTGTTATGGCAGGGGACGCTGCACTGGCCCTTGA
  GAGCATCGTGAGCATGGCCGTGTTTGACGAGCCTCCACCAGACATTACCACCTGGCCTCTTTTGGAAGGC
  CAAGAGACCCCTGCCGCGAGAGCCGGGGCTGTGGGCGCCTATCTCGCCCGGGCAGCTGGCCTGGTGGGGG
  CAATGGTGTTTAGTACCAACAGCGCCCTGCACCTGACTGAAGTGGATGACGCAGGCCCTGCTGACCCAAA
  AGACCACTCTAAGCCTTCATTTTACAGGTTTTTCTTGGTGCCCGGAACCCATGTCGCCGCCAACCCACAG
  CTGGACCGGGAGGGTCATGTGGTGCCTGGGTATGAGGGAAGGCCAACCGCCCCCCTCGTGGGAGGGACAC
  AGGAGTTTGCCGGGGAGCATCTGGCAATGTTGTGTGGCTTTAGCCCAGCCTTGCTGGCTAAGATGCTGTT
  TTACCTGGAACGCTGCGATGGTGGGGTCATTGTCGGTCGCCAGGAAATGGACGTGTTTCGCTACGTCGCA
  GATTCCGGCCAGACCGACGTTCCCTGTAATCTGTGTACCTTTGAGACGCGACATGCTTGTGCGCACACAA
  CCCTCATGAGGCTGCGCGCTAGACACCCAAAGTTCGCCTCAGCGGCACGGGGGGCAATTGGGGTGTTTGG
  CACGATGAACAGCGCTTATTCTGATTGCGACGTTCTGGGTAATTACGCCGCTTTCTCCGCTCTTAAGCGC
  GCTGATGGGTCAGAGAACACCCGGACAATCATGCAGGAGACCTATCGCGCTGCAACTGAAAGGGTAATGG
  CCGAGCTGGAGGCACTGCAATACGTTGACCAGGCTGTACCCACTGCGCTCGGAAGACTTGAGACCATCAT
  TGGGAATCGCGAAGCCCTGCATACAGTTGTGAACAATATCAAACAGCTCGTTGATCGGGAAGTAGAACAG
  CTGATGCGGAACCTCATCGAGGGCAGAAATTTTAAGTTCAGAGACGGCCTCGCTGAGGCCAACCACGCTA
  TGAGCCTTAGCCTGGACCCATACACATGTGGCCCTTGTCCCCTTCTGCAGCTGCTGGCAAGAAGATCAAA
  CCTGGCTGTCTATCAGGACTTGGCTCTCTCCCAGTGTCATGGCGTGTTTGCTGGTCAGAGTGTCGAAGGC
  CGGAATTTTCGAAATCAGTTCCAGCCCGTGCTGAGAAGGCGAGTCATGGATCTCTTTAACAACGGATTCC
  TGAGCGCGAAAACACTGACTGTTGCTTTGTCTGAAGGGGCCGCCATCTGTGCGCCCAGCCTGACAGCTGG
  ACAAACCGCACCTGCTGAATCCAGCTTTGAGGGCGATGTTGCGCGGGTAACATTGGGATTTCCTAAGGAA
  CTTAGGGTCAAGTCTAGGGTGCTCTTCGCTGGCGCAAGCGCCAACGCTAGTGAGGCAGCCAAGGCCCGCG
  TTGCATCCCTCCAGTCTGCCTACCAGAAACCCGACAAACGAGTTGATATTTTGCTTGGCCCGCTTGGTTT
  CCTCCTGAAGCAGTTCCACGCCGTGATATTCCCCAATGGTAAGCCGCCAGGCAGTAACCAGCCAAACCCC
  CAGTGGTTCTGGACCGCTCTCCAGCGCAACCAGCTGCCTGCACGGCTCCTGAGCCGCGAGGATATAGAGA
  CAATTGCTTTTATCAAAAGATTTAGCCTCGACTACGGCGCAATAAACTTTATTAATCTTGCCCCTAATAA
  CGTGTCTGAGCTGGCTATGTACTATATGGCCAATCAGATTCTGAGATATTGCGACCATAGCACCTACTTC
  ATCAACACTCTGACAGCCGTGATCGCCGGCTCCAGACGGCCTCCTTCTGTTCAAGCCGCCGCTGCCTGGG
  CTCCACAAGGTGGAGCCGGTCTGGAAGCAGGAGCTCGGGCACTTATGGACTCACTCGACGCTCATCCCGG
  CGCCTGGACTTCAATGTTTGCAAGCTGTAACCTGCTCCGACCTGTGATGGCTGCACGGCCGATGGTGGTG
  TTGGGGCTGTCCATTAGTAAGTATTATGGCATGGCCGGAAACGATAGGGTCTTTCAAGCTGGAAACTGGG
  CTAGCCTGCTGGGAGGCAAGAATGCTTGTCCATTGCTGATTTTTGACCGAACGCGGAAGTTTGTCCTCGC
  TTGTCCAAGGGCCGGGTTCGTCTGTGCCGCCTCATCCTTGGGCGGCGGCGCCCATGAGCACTCCCTTTGT
  GAACAGCTGAGAGGGATTATTGCCGAGGGAGGGGCCGCAGTGGCATCCAGCGTGTTTGTGGCTACTGTTA
  AATCACTTGGTCCTAGAACCCAGCAACTCCAGATTGAAGACTGGCTGGCCCTTTTGGAAGATGAATACCT
  GTCAGAGGAAATGATGGAGTTCACGACAAGAGCTCTGGAACGGGGCCACGGCGAGTGGAGTACCGACGCC
  GCCCTTGAGGTCGCTCACGAAGCTGAGGCCTTGGTGAGCCAGCTTGGGGCTGCCGGCGAGGTCTTCAACT
  TTGGAGATTTCGGCGACGAAGATGACCATGCCGCAAGTTTTGGGGGACTGGCCGCGGCAGCAGGCGCCGC
  AGGTGTGGCCCGAAAACGCGCTTTCCACGGCGATGATCCTTTCGGGGAGGGGCCACCTGAAAAGAAAGAC
  TTGACTTTGGACATGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100894 representing NP_044499
  Red=Cloning sites Green=Tags
  
  MDTKPKTTTTVKVPPGPMGYVYGRACPAEGLELLSLLSARSGDADVAVAPLIVGLTVESGFEANVAAVVG
  SRTTGLGGTAVSLKLMPSHYSPSVYVFHGGRHLAPSTQAPNLTRLCERARPHFGFADYAPRPCDLKHETT
  GDALCERLGLDPDRALLYLVITEGFREAVCISNTFLHLGGMDKVTIGDAEVHRIPVYPLQMFMPDFSRVI
  ADPFNCNHRSIGENFNYPLPFFNRPLARLLFEAVVGPAAVALRARNVDAVARAAAHLAFDENHEGAALPA
  DITFTAFEASQGKPQRGARDAGNKGPAGGFEQRLASVMAGDAALALESIVSMAVFDEPPPDITTWPLLEG
  QETPAARAGAVGAYLARAAGLVGAMVFSTNSALHLTEVDDAGPADPKDHSKPSFYRFFLVPGTHVAANPQ
  LDREGHVVPGYEGRPTAPLVGGTQEFAGEHLAMLCGFSPALLAKMLFYLERCDGGVIVGRQEMDVFRYVA
  DSGQTDVPCNLCTFETRHACAHTTLMRLRARHPKFASAARGAIGVFGTMNSAYSDCDVLGNYAAFSALKR
  ADGSENTRTIMQETYRAATERVMAELEALQYVDQAVPTALGRLETIIGNREALHTVVNNIKQLVDREVEQ
  LMRNLIEGRNFKFRDGLAEANHAMSLSLDPYTCGPCPLLQLLARRSNLAVYQDLALSQCHGVFAGQSVEG
  RNFRNQFQPVLRRRVMDLFNNGFLSAKTLTVALSEGAAICAPSLTAGQTAPAESSFEGDVARVTLGFPKE
  LRVKSRVLFAGASANASEAAKARVASLQSAYQKPDKRVDILLGPLGFLLKQFHAVIFPNGKPPGSNQPNP
  QWFWTALQRNQLPARLLSREDIETIAFIKRFSLDYGAINFINLAPNNVSELAMYYMANQILRYCDHSTYF
  INTLTAVIAGSRRPPSVQAAAAWAPQGGAGLEAGARALMDSLDAHPGAWTSMFASCNLLRPVMAARPMVV
  LGLSISKYYGMAGNDRVFQAGNWASLLGGKNACPLLIFDRTRKFVLACPRAGFVCAASSLGGGAHEHSLC
  EQLRGIIAEGGAAVASSVFVATVKSLGPRTQQLQIEDWLALLEDEYLSEEMMEFTTRALERGHGEWSTDA
  ALEVAHEAEALVSQLGAAGEVFNFGDFGDEDDHAASFGGLAAAAGAAGVARKRAFHGDDPFGEGPPEKKD
  LTLDML
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 3588 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044499
• RefSeq ORF: 3588
• Locus ID: 1487314
• MW: 128.4 kDa