US5 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100930

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein J [Human herpesvirus 2], codon optimized for human cell expression, NP_044535

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100930 represents NCBI reference of NP_044535 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGATACGCAGTCAGAACATGGGGAATTGTCGGGATTCTGGGATGTGCCGCCGTCGGTGCCGCTC
  CCACTGGTCCGGCATCCGATACCACAAATGCGACCGCACGATTGCCAACTCACCCACCTCTGATCCGGTC
  CGGGGGATTTGCCGTACCTCTTATCGTGGGGGGTCTGTGTCTCATGATTCTGGGAATGGCCTGCCTGCTG
  GAGGTACTGCGGCGACTCGGTAGAGAGCTGGCCCGCTGCTGTCCCCACGCGGGTCAGTTCGCGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100930 representing NP_044535
  Red=Cloning sites Green=Tags
  
  MDRYAVRTWGIVGILGCAAVGAAPTGPASDTTNATARLPTHPPLIRSGGFAVPLIVGGLCLMILGMACLL
  EVLRRLGRELARCCPHAGQFAP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 276 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044535
• RefSeq ORF: 276
• Locus ID: 1487357
• MW: 9.5 kDa