US6 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100931

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein D [Human herpesvirus 2], codon optimized for human cell expression, NP_044536

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100931 represents NCBI reference of NP_044536 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGACTGACGTCAGGTGTGGGCACCGCTGCTCTCCTCGTGGTCGCAGTCGGGCTCAGGGTTGTGT
  GCGCTAAGTACGCGCTGGCAGACCCTAGTCTCAAAATGGCTGACCCGAACCGGTTTCGCGGTAAAAACCT
  CCCAGTTCTGGACCAGCTGACAGACCCCCCCGGCGTGAAGAGGGTCTATCATATTCAGCCATCTCTGGAG
  GACCCTTTTCAGCCCCCCTCCATTCCTATCACCGTGTACTACGCAGTGCTCGAACGCGCCTGTAGAAGCG
  TCCTGCTTCATGCACCCTCTGAAGCCCCACAGATCGTACGGGGGGCCTCTGATGAGGCTCGGAAACACAC
  GTACAATCTGACAATAGCATGGTATAGGATGGGCGATAATTGCGCGATACCTATTACCGTGATGGAATAC
  ACGGAATGCCCATATAATAAGAGTCTCGGCGTGTGCCCGATCAGGACACAGCCTCGGTGGTCCTACTATG
  ATAGTTTTAGCGCAGTGTCAGAAGACAATCTTGGCTTCCTCATGCATGCCCCCGCATTTGAGACTGCGGG
  AACTTACCTTAGGCTTGTGAAAATTAATGATTGGACAGAGATCACGCAGTTCATCCTGGAGCACCGAGCT
  AGGGCGAGTTGTAAGTACGCTCTGCCCCTGCGCATCCCACCTGCCGCCTGCCTGACCTCCAAAGCTTATC
  AACAAGGGGTCACGGTGGACAGCATCGGCATGCTCCCCAGATTTATTCCAGAGAACCAGCGCACTGTCGC
  TCTCTACTCACTGAAAATTGCCGGATGGCACGGTCCAAAGCCCCCATACACTTCAACATTGCTGCCGCCA
  GAATTGAGCGATACTACAAATGCCACTCAGCCTGAGCTTGTCCCCGAGGATCCAGAGGATTCTGCCCTGC
  TCGAGGATCCAGCCGGGACCGTATCTTCCCAGATCCCTCCTAACTGGCACATCCCCAGTATTCAGGATGT
  AGCACCCCATCATGCCCCAGCCGCGCCAAGCAATCCAGGTCTCATTATCGGCGCCCTCGCCGGCTCTACA
  CTTGCCGTGCTGGTAATCGGTGGTATTGCTTTCTGGGTCCGCCGAAGAGCTCAGATGGCACCTAAACGCC
  TCCGGTTGCCACATATTAGAGACGATGATGCACCCCCAAGCCATCAGCCCCTGTTTTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100931 representing NP_044536
  Red=Cloning sites Green=Tags
  
  MGRLTSGVGTAALLVVAVGLRVVCAKYALADPSLKMADPNRFRGKNLPVLDQLTDPPGVKRVYHIQPSLE
  DPFQPPSIPITVYYAVLERACRSVLLHAPSEAPQIVRGASDEARKHTYNLTIAWYRMGDNCAIPITVMEY
  TECPYNKSLGVCPIRTQPRWSYYDSFSAVSEDNLGFLMHAPAFETAGTYLRLVKINDWTEITQFILEHRA
  RASCKYALPLRIPPAACLTSKAYQQGVTVDSIGMLPRFIPENQRTVALYSLKIAGWHGPKPPYTSTLLPP
  ELSDTTNATQPELVPEDPEDSALLEDPAGTVSSQIPPNWHIPSIQDVAPHHAPAAPSNPGLIIGALAGST
  LAVLVIGGIAFWVRRRAQMAPKRLRLPHIRDDDAPPSHQPLFY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044536
• RefSeq ORF: 1179
• Locus ID: 1487358
• MW: 43.2 kDa